Labshake search
Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Mice were euthanized on day 15 and tumors were collected and digested in RPMI + 10% FBS with 20 μg/ml DNase I type IV (Sigma-Aldrich) and 1 mg/ml Collagenase from Clostridium histolyticum (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... and then incubated in a shaking water bath (180 rpm) at 37°C for 50 minutes with Type 1 Collagenase (2 mg/ml; Sigma-Aldrich) and DNase (100 U/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2023Quote: ... the hydrophobic modification of microfluidic channels was performed by flushing both types of devices with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma-Aldrich) in HFE-7500 (3M ...
-
bioRxiv - Immunology 2023Quote: ... The tissue was incubated in RPMI/5%FCS/10mMHEPES for 5min before being digested in 10ml RPMI/5% FCS/10mM HEPES supplemented with 0.4mg/ml type VIII collagenase (Sigma Aldrich, UK) and 40μg/ml DNase I (Roche ...
-
bioRxiv - Genetics 2023Quote: The flexor digitorum brevis (FDB) and interosseous (IO) muscles were freshly isolated from mice and incubated with collagenase (Sigma type 1) for 1 hr at 37 °C in a Tyrode solution ...
-
bioRxiv - Biophysics 2023Quote: ... under mild stirring in 16 mL of SCL containing 100 U/mL of collagenase (type II, from Clostridium histolyticum, Sigma C6885) and 1 mM CaCl2 ...
-
bioRxiv - Cancer Biology 2023Quote: The human OS cell line 143B was purchased from the American Type Culture Collection (ATCC, Manassas, USA) and cultured in Eagle’s minimum essential medium (EMEM; Sigma Aldrich, UK), supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... and seed on elastic cell culture substrates with defined stiffness (2, 15 or 100kPa) (ExCellness) coated with type I collagen (Sigma-Aldrich) and were covered with the organoid medium ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were seed on elastic cell culture substrates with defined stiffness (2, 15 or 100kPa) (ExCellness) coated with type I collagen (Sigma-Aldrich). Matrigel on top (MoT ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were purchased from American Type Cell Culture (ATCC, USA) and cultured in ATCC-recommended media supplemented with 10% FBS (Sigma-Aldrich) and 1% pen/strep (100units/ mL penicillin ...
-
bioRxiv - Bioengineering 2023Quote: ... d15 organoids were sequentially incubated for one hour with collagenase solution (PBS +Ca/Mg, 0.25% Collagenase Type I (Sigma-Aldrich, #C0130), 20% FBS (Thermo Fisher ...
-
bioRxiv - Physiology 2023Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatine type B (Sigma-Aldrich). Explants were maintained in RPMI ...
-
bioRxiv - Immunology 2023Quote: ... Then after having carried out a pre-isolation on a 40%-23%-20%-11% ficoll gradient (Sigma Ficoll type 400, F4375), the islets were hand picked and maintained overnight à 37°C ...
-
bioRxiv - Immunology 2023Quote: ... After infusion of the pancreas via the common bile duct with 5 ml of collagenase 1 mg/ml (type XI, Sigma C7657), they were removed and placed in RPMI 1640 supplemented with 2 ml of collagenase solution and digested for 7 min using a 37°C water bath ...
-
bioRxiv - Neuroscience 2024Quote: ... re-hydrated in PB and mounted in gelatinized slides (gelatine type a g8-500, Fisher Chemical) using Mowiol as mounting medium (81381, Sigma-Aldrich).
-
bioRxiv - Cell Biology 2024Quote: ... the center of the glass dish was coated with 6% Concanavalin A (Canavalia ensiformis Jack Bean, type IV Sigma C2010-G) for 10 minutes ...
-
bioRxiv - Biophysics 2024Quote: ... which includes DMEM media with the final concentration of 0.56 mg/mL of glucose oxidase (Aspergillus niger-Type VII, lyophilized powder, Sigma-Aldrich, G2133) and 34 μg/mL of catalase (bovine liver-lyophilized powder ...
-
bioRxiv - Cancer Biology 2024Quote: MDA-MB-231 cell line was purchased from the American Type Culture Collection (ATCC HTB-26) and cultured in RPMI-1640 medium (Millipore Sigma) supplemented with 10% FBS (Millipore Sigma) ...
-
bioRxiv - Biochemistry 2024Quote: ... a general HDAC fluorometric deacetylase enzyme assay was used to determine total HDAC activities relative to wild-type U251-MG cells from various HDAC-immunoprecipitates (Sigma CS1010). To each well of a 384-well plate ...
-
bioRxiv - Immunology 2024Quote: ... eWAT fat pads were digested in 5 mL digestion buffer containing 1 mg/mL collagenase type II from Clostridium histolyticum (Sigma-Aldrich) in Krebs buffer supplemented with 100 mM HEPES ...
-
bioRxiv - Biophysics 2024Quote: ... structures were mechanically dissociated with a pipette (plastic tip 1 mm diameter) in presence of DNAse type IV (20U/mL, Sigma D5025). Cells viability was determined by Trypan Blue exclusion assay ...
-
bioRxiv - Cell Biology 2024Quote: ... Perfused whole lungs were diced using surgical scissors and digested in a Phosphate Buffered Saline (PBS; Mg-Ca-) solution supplemented with 5mg/mL Collagenase Type I (Thermo) and 50 units of DNase (Millipore Sigma) at 37°C for 1 hour with frequent mechanical perturbation ...
-
bioRxiv - Microbiology 2024Quote: Dose-dependent effects of mucins on IAV cell-to-cell spread were studied by plaque assay in presence of mucin from porcine stomach type III (M1778, Sigma-Aldrich) in a concentration range of 0-2% (w/v) ...
-
bioRxiv - Microbiology 2024Quote: ... along with the original content of lactose (6.4 g/L) and mucin (4 g/L porcine gastric type II mucin, Sigma-Aldrich, Germany). The total MO level was adjusted to quantities reported in mature porcine milk31 ...
-
bioRxiv - Cell Biology 2024Quote: Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords using collagenase type I (Sigma-Aldrich, 400 U/mL) to separate the cell layer surrounding the vein lumen ...
-
bioRxiv - Molecular Biology 2024Quote: SPL life sciences cell culture dishes (cat# 20060) were coated with 6 μg/cm2 of collagen from human placenta Bornstein and traub type IV (Sigma C5533) in hanks’ balanced salt solution ((- ...
-
bioRxiv - Biochemistry 2024Quote: While attached to 1D4 resin, receptors were incubated with phosphatidylcholine (POPC, Avanti) and bovine brain lipid (BBL) extract (type I, Folch fraction I, Sigma-Aldrich) mixture (POPC:BBL = 85:15 ...
-
Ion channel function of polycystin-2/polycystin-1 heteromer revealed by structure-guided mutagenesisbioRxiv - Biophysics 2024Quote: ... The isolated sections of the ovarian lobes were then treated with 600-700 U/ml collagenase type-2 from Clostridium hystolyticum (Sigma-Aldrich) for 3-4 hours at 19°C ...
-
bioRxiv - Immunology 2024Quote: ... The majority of the digestion RPMI was removed and 10 mL of digestion RPMI supplemented with 0.1 mg/mL Collagenase Type VIII (Sigma, Cat. # C2139) and 0.075 U/mL Dispase (Corning ...
-
bioRxiv - Bioengineering 2024Quote: The alkaline comet assay was performed by mixing 1.5 × 105 cells 1:1 with 2 % low melting point agarose (2-Hydroxyethylagarose, Type VII, Sigma-Aldrich, A4018) at 37°C ...
-
bioRxiv - Bioengineering 2024Quote: ... 100 μl of this suspension was placed on a microscope slide pre-coated with 0.5 % high melting point agarose (Type I-A, Sigma-Aldrich, A0169), covered with a coverslip and put at 4°C to solidify for 5 min ...
-
bioRxiv - Immunology 2024Quote: ... The apical and basal surfaces of transwell filters were coated with 50 µl of Collagen Type I (50 µg/mL; CC050, Sigma-Aldrich), 50 µl of Collagen Type IV (50 µg/mL ...
-
bioRxiv - Immunology 2024Quote: ... The synovial tissue was chopped into small pieces and digested overnight at 37°C with 1mg/mL type IV collagenase (Merck Millipore, USA) mixed in Dulbecco’s Modified Eagle Medium (DMEM) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cells were purchased from American Type Cell Culture (ATCC, USA) and cultured in ATCC-recommended media supplemented with 10% FBS (Sigma-Aldrich) and 1% pen/strep (100units/ mL penicillin ...
-
bioRxiv - Bioengineering 2024Quote: ... the tendons from mice tail were isolated at 28 days of age and digested in type I collagenase solution (2mg/mL in 1X PBS, Sigma C0130) by inverting every 10 minutes for one hour in a 37 °C incubator ...
-
bioRxiv - Biophysics 2024Quote: ... and incubated on shaker at 35°C for 30 min in the same solution with the addition of elastase type IV (0.6 mg/ml; Sigma, Chemical Co.), collagenase type 2 (0.8 mg/ml ...
-
bioRxiv - Cell Biology 2024Quote: ... was carried out at designated time points in a cryo-protectant solution (PBS with 2% Type IX ultra-low melt agarose (Sigma-Aldrich), 100 mM D-mannitol ...
-
bioRxiv - Cell Biology 2024Quote: ... 1cm2 of back skin from 8-14 weeks old female wild-type and knockout littermates was dissociated in 0.25 mg/mL Liberase (Sigma, cat. #5401020001) and 0.5 mg/mL DNase (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were allowed to rest for 3 min before being plated in Cultrex BME Type 2 RGF Pathclear and cultured in basal media containing Rho kinase inhibitor Y-27632 (Sigma, Y0503). Media was changed the following day to basal media only ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor fragments were performed to enzymatic digestion in advanced DMEM/F12 supplemented with 1x Glutamax and 10 mM HEPS buffer containing collagenase type IV (200 U/ml, Sigma–Aldrich) and DNase I (50 U/ml ...
-
bioRxiv - Cell Biology 2020Quote: ... were fixed with warm 2% PFA in PBS containing 0.4% w/v sucrose for 12 minutes at room temperature and quenched with 0.25% ammonium chloride (SIGMA) for 10min ...
-
bioRxiv - Cell Biology 2020Quote: ... in PBS containing 0.4% w/v Sucrose for 12 minutes at room temperature and quenched with 0.25% Ammonium Chloride (SIGMA) for 10 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... a fraction of Proteins was separated on 12% SDS-PAGE gels and transferred to Immobilon PVDF membranes (Millipore). The membranes were blocked in 5% milk or 5% BSA (depending on the primary antibody) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1□mM MgCl2, 0.2□mM Na2HPO4, 12□mM NaHCO3, 5.5□mM D-glucose, 1.8□mM CaCl2; Sigma–Aldrich) at physiological conditions (37°C ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated for 3.5 h in complete IMDM medium containing 0.1 μM phorbol 12-myristate 13-acetate (P-8139 Sigma) and 1 μM ionomycin (I-0634 Sigma ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 mM MgCl2, 0.2 mM Na2HPO4, 12 mM NaHCO3, 5.5 mM d-glucose, and 1.8 mM CaCl2; Sigma-Aldrich) for at least 30min at 37°C and then imaged on a Zeiss Axio Observer Z1 inverted microscope equipped with a Hamamatsu ORCA-Flash 4.0 sCMOS camera ...
-
bioRxiv - Developmental Biology 2020Quote: ... Isolated DFs were seeded in DMEM/F-12 medium containing 15% FBS and antibiotics (penicillin/streptomycin, Sigma-Aldrich). All in vitro experiments were performed on primary (p=0 ...
-
bioRxiv - Immunology 2022Quote: ... Supernatants were harvested on day 12 post-transfection for antibody purification using rProtein A Sepharose affinity chromatography (Sigma).