Labshake search
Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: Nuclear proteins were extracted from frozen poplar stem using the CellLytic™ PN kit (Sigma, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: Detection of an interaction between proteins was assessed using the Duolink II Detection Kit (Sigma-Aldrich) according to the manufacturer specifications ...
-
bioRxiv - Molecular Biology 2024Quote: RIP assays were performed using the Magna RIPTM RNA-binding protein immunoprecipitation kit (Millipore, Massachusetts, USA). Cells in 15 cm dishes were lysed in complete RIP lysis buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitated protein-DNA complexes were prepared using the Magna ChIP A kit (Millipore, Burlington, MA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: Total protein content in all cell extracts was determined using the QuantiPro BCA Assay Kit (Sigma).
-
bioRxiv - Developmental Biology 2023Quote: ... detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma). Fg-Stk36WT and Fg-Stk36AA were immunopurified from HEK293 cells transfected with the Fg-Stk36WT or Fg-Stk36AA construct.
-
bioRxiv - Immunology 2022Quote: A 96 well ELISA plate was coated with bovine fetuin (5 μg/mL; Sigma) in carbonate buffer overnight ...
-
bioRxiv - Microbiology 2019Quote: ... supernatants were collected and analyzed for total GLP-1 levels by ELISA (Millipore Sigma) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
-
bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
-
bioRxiv - Microbiology 2024Quote: ELISA plates were coated with 1mg⍰mL of chondroitin sulphate A (CSA) (Sigma, C8529) in PBS (Gibco ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
-
bioRxiv - Biochemistry 2024Quote: ... 2 mM 2-Mercaptoethanol (Sigma-Aldrich; Cas: 60-24-2), and 4 mM 4-Nitrophenyl acetate (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: mRNA was purified from total RNA with 2 rounds of GeneElute mRNA purification kit (Sigma). rRNA was removed using Ribominus kit (Invitrogen ...
-
Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... and Phospho/Total ERK 2-Plex Magnetic Bead Kit (EMD Millipore Corporation, Billerica, MA, U.S.A.) were used to assess the levels of these proteins ...
-
bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
-
bioRxiv - Genetics 2021Quote: ... purified as previously described18) or HiFi-3xNLS-SpCas9 protein (100 pmol) and sgRNA (300 pmol, IDT) with glycerol (2% of final concentration, Sigma, G2025) and incubating for 15 min at room temperature immediately before electroporation ...
-
bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore ...
-
bioRxiv - Immunology 2020Quote: ... Thirty micrograms (30µg) of total protein from each sample was then reduced with 10mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA) at 55°C for 1h and subsequently alkylated with 18mM IAA (Sigma-Aldrich ...
-
bioRxiv - Immunology 2020Quote: ... Thirty micrograms (30µg) of total protein from each sample was then reduced with 10mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, USA) at 55°C for 1h and subsequently alkylated with 18mM IAA (Sigma-Aldrich ...
-
The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
-
bioRxiv - Cell Biology 2021Quote: ... proteins were extracted from 2 million cells unstimulated or stimulated with LPS or MSUc supplemented with protease inhibitors (Sigma Aldrich, #11697498001) Protein concentration was measured using the BCA reagent (Biorad ...
-
bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
-
bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
-
bioRxiv - Genetics 2019Quote: FLAG-tagged proteins were immunoprecipitated from 2 mg of HEK293T or M17 cells using anti-FLAG M2 affinity gel (Sigma A2220). Lysates were prepared in NP40 buffer (see Western blotting) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The loading solution consisted of 15 µL of the protein sample mixed with 1 µL DTT and 15 µL Laemmli 2× Concentrate Loading Buffer (Sigma-Aldrich), and denatured at 95 °C for 10 min ...
-
bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
-
bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
-
bioRxiv - Microbiology 2021Quote: The heat extracted proteins from OD600=2 culture was suspended in 1X Laemmli buffer with 250 U of benzonase nuclease (Sigma E0114) and incubated for 5 minutes at 95°C ...
-
bioRxiv - Neuroscience 2020Quote: ... to preclear samples for 2 hours and subsequently incubated with Dynabeads protein G coated with an anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10 μg of protein was reduced with 10 mM dithiothreitol (DTT; FUJIFILM Wako) and alkylated with 40 mM 2-chloroacetamide (CAA; Sigma-Aldrich). Digestion was performed according to the PTS protocol (Chang et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... 500 µg of lysate proteins were incubated with 2 µl of anti-Flag monoclonal antibody pre-adsorbed on protein A-Sepharose beads (cat. no. A2220, Sigma-Aldrich) overnight at 4°C.
-
bioRxiv - Molecular Biology 2022Quote: ... Soluble proteins were hatched for 10 min at 55°C with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, St. Louis, MO, USA), then alkylated for 15 min at RT in the dark with 10 mg/mL iodoacetamide (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... Expression of the different FLAG-SET8 proteins in U2OS cells were induced by the addition of 2 µg/ml of tetracyclin (TET) (Sigma-Aldrich) into cell culture medium ...
-
bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... 80 µg of equivalent proteins from sample were treated with 5mM tris (2-carboxyethyl) phosphine hydrochloride (Sigma-Aldrich Cat No: C4706) for 15 minutes at 37°C to reduce the disulfide bonds ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked and permeabilized in protein-blocking buffer (DPBS with 2% BSA, 10% fish gelatin (cat#: G7041, Sigma-Aldrich), 0.2% Triton-X (cat# T9284 ...
-
bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...
-
bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase conjugated secondary antibodies (table 2) were added for 1 h at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cy3-mouse-anti-mouse aSMA (Sigma C6198), mouse anti-mouse αSMA (Sigma A2547) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
-
bioRxiv - Neuroscience 2021Quote: ... The isotype of RNF5 was determined using the IsoStrip Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich) and found to be IgG2b with a kappa light chain.
-
bioRxiv - Bioengineering 2019Quote: ... The isotype of lead clone was determined using mouse antibody isotyping kit (Sigma, St. Louis, MO).
-
bioRxiv - Microbiology 2021Quote: ... was detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich; #DUO92101-1kit) according to the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2020Quote: ... PLA was carried out using Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma, DUO92101) with 1:500 biotin primary antibody (Abcam ...
-
bioRxiv - Immunology 2020Quote: ... Luminex multiplex assays were performed using the mouse Ig isotyping kit (MGAMMAG-300K, Millipore, Burlington MA) and conducted according to the manufacturer’s instructions by the Analytical Pharmacology Core at City of Hope.
-
Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: PLA was carried out using the Duolink InSitu Orange Starter Kit Mouse/Rabbit (DUO92102, Sigma-Aldrich) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2023Quote: Proximity ligation assay was performed using a Duolink in situ Red Starter Kit Mouse/Rabbit (Sigma). Following antibodies were used ...
-
bioRxiv - Physiology 2023Quote: ... using the Milliplex® Mouse Cytokine Kit (#MCYTOMAG-77k, Merk Millipore, Germany, intra-assay %CV <10%). The kit enabled the quantification of IL-1α ...
-
bioRxiv - Neuroscience 2021Quote: Protein lysates were extracted from the hippocampi or cortices of adult mice and dissected in ice-cold PBS containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and Mini-Complete Protease Inhibitor Cocktail (Roche Diagnostics ...