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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Biophysics 2022Quote: ... Five microliters of a 5 mg mL−1 solution of protein G (Sigma-Aldrich, Tokyo, Japan) was added to the flow chamber (about 5 μL) ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein production was induced by adding 1 mM IPTG and 10 µM all-trans retinal (Sigma) at an absorbance of 0.8–0.9 at 600 nm ...
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bioRxiv - Genetics 2022Quote: ... hPSC-CMs were stained with sarcomreirc protein TNNT2 (Catalog #: HPA017888 Lot #: A91786, Sigma, dilution 1/200), secondary antibody Goat anti-Rabbit IgG Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins synthesized in this assay were detected using anti-FLAG antibodies (F3165 SIGMA, 1:4000 dilution) and the Anti-mouse IgG ...
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bioRxiv - Physiology 2019Quote: ... was from BD Biosciences; citrate synthase (CS; SAB2701077) and mitochondrial fission protein 1 (FIS1; HPA017430) were from Sigma Aldrich; vitamin D receptor (D-6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM of isopropyl-β-D-thiogalactoside (IPTG) (Sigma Aldrich). Expression was continued in a bacterial shaker at 30°C and 150 rpm for 6h ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Cell Biology 2020Quote: ... the culture medium was supplemented with 1 μM Rho-associated protein kinase (ROCK) inhibitor (Y27632, Millipore). Recombination was induced with 100 nM 4-hydroxytamoxifen (4OHT ...
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bioRxiv - Cell Biology 2021Quote: ... 30 μg of proteins were mixed with 0.004% Bromophenol blue and 1% β-Mercaptoethanol (Sigma-Aldrich), incubated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 140μg of mitochondrial protein was incubated with 1:100 of 1M dithiothreitol (Sigma-Aldrich, SKU 43815), followed by 1:50 dark incubation in 1M iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... with the protein aqueous solution (1 mg/ml of PLL, Sigma-Aldrich, in PBS pH 10.4), at a 1:2 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... and bands were normalized to total protein and the housekeeping gene Gapdh (1:10,000, Millipore, NP_002037).).
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Cell Biology 2023Quote: ... 35 μg of proteins were supplemented with 0.004% Bromophenol blue and 1% β-mercaptoethanol (Sigma-Aldrich), incubated at 95 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 10k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 3k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 1% FBS (v/v) and protein expression enhancing sodium butyrate (65) solution (SIGMA-ALDRICH) to a final concentration of 2 nM were added to the cells to make up to the final volume of transfection ...
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bioRxiv - Biophysics 2024Quote: ... and when necessary concentrated the protein to 1∼2 mg/ml (Amicon Ultra 100 KDa, Millipore). For experiments under K+-free conditions ...
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bioRxiv - Neuroscience 2019Quote: ... P0 rat hippocampal neurons were plated to poly-D-lysine (PDL; 10μg/cm2; Millipore Cat# A-003-E) coated coverslips at 5×104 neurons per well in a 24-well plate ...
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bioRxiv - Microbiology 2020Quote: ... or spectinomycin (100 mg/L for E. coli or 400 mg/L for S. venezuelae, Sigma Aldrich, USA) All bacterial strains and plasmids used in this work are listed in Table 2 and are available through the public instance of the JBEI registry ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Microbiology 2021Quote: The anti-flavivirus E monoclonal antibody (mAb) D1-4G2-4-15 was purchased from Millipore (Billerica, MA, USA). The rabbit anti-ZIKV E polyclonal antibody was purchased from GeneTex (Alton PkwyIrvine ...
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bioRxiv - Immunology 2019Quote: ... Cells were either unstimulated or challenged with LPS (1ug/mL; LPS from E. coli O111:B4; Sigma-Aldrich) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... The primers for PKC isoforms and E-cadherin were adopted from Awadelkarim et al.iv and Labernadie et al.v and ordered from Sigma. Prior to all experiments ...
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bioRxiv - Cell Biology 2020Quote: ... Spread screen samples were infected with VACV E-EGFP L-mCherry at MOI 0.02 and infection media was changed to media containing AraC (10µM, Sigma) at 8 hpi.
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bioRxiv - Immunology 2021Quote: ... DCs were treated for 18h with Fh12 (2.5µg/ml or 1µg/ml LPS (E. coli 0111: B4; Sigma-Aldrich). In the inhibition experiments ...
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bioRxiv - Genetics 2020Quote: ... 20 mM MgSO4, 200 mM HEPES, pH 7.9), protease inhibitor cocktail (AEBSF, 1,10-Phenanthroline, Pepstatin A, E-64) (Sigma), and TritonX-100 ...
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bioRxiv - Immunology 2020Quote: ... M1 macrophages were then kept unstimulated or stimulated with 100 ng/mL LPS (E. coli 0111:B4; Sigma) for 4h or 8h ...
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bioRxiv - Cell Biology 2023Quote: ... this was done by incubating embryos right after fertilization in 1.5 mg/mL Pronase E (Sigma-Aldrich, #107433) for approximately five minutes ...
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bioRxiv - Immunology 2023Quote: ... Routine hematoxylin and eosin (H&E) stainings and Periodic Acid Schiff (PAS) staining (Millipore-Sigma, catalog #395B-1KT) were performed per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Routine hematoxylin and eosin (H&E) stainings and Periodic Acid Schiff (PAS) staining (Millipore-Sigma, catalog #395B-1KT) were performed per manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... sectioned and stained with hematoxylin and eosin (H&E) or immunohistochemically stained for Tyrosine Hydroxylase (ab152, Millipore Sigma) or UCP1 (ab10983 ...
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bioRxiv - Bioengineering 2023Quote: dPPM was stained with H&E (Hematoxylin solution, Gill no.2, Sigma Aldrich, aqueous Eosin Y, Thermo scientific) to assess cellular content and histoarchitecture ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections of 4 µm were stained with haematoxylin and eosin (H&E, Sigma-Aldrich, St. Louis, MO, EUA) or antibodies against fibronectin (ab2413 ...
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bioRxiv - Cell Biology 2023Quote: ... 300,000 to 400,000 NPCs seeded into microscope coverslips were treated with 100nM compound E (EMD Millipore, Temecula, CA) in BrainPhys medium (StemCell Technologies ...
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bioRxiv - Pathology 2023Quote: Hair follicle parameters were stained with H&E (H3136 Sigma-Aldrich Hematoxylin and E4009 Sigma-Aldrich Eosin Y). Dermis-dermal adipocyte layer thickness and the numbers of hair follicles and anagen stages were counted in three areas (9 sections ...