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Citations for Millipore Sigma :
2001 - 2050 of 2589 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... For primary antibodies we used hybridoma supernatant of monoclonal antibody 6E3 Rat anti-CLASP2 (Maffini et al., 2009) at 1:150 and Mouse anti-α-tubulin clone B-512 (Sigma Aldrich) at 1:10000 ...
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bioRxiv - Microbiology 2019Quote: ... were maintained at 37°C on rat collagen diluted at 100µg/mL in water (Cultrex; catalog no. 3443-100-01) in EndoGro medium (Merck Millipore; catalog no. SCME004) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated at 14 °C (a temperature not permissible for recycling) with unconjugated goat anti-rat IgG (Sigma, 4 μg/ml) to block labelled channels remaining at the cell surface ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated with the primary antibody (rat anti-serotonin, Chemicon MAB352, 5/1000; rabbit anti-tyrosine hydroxylase, Sigma Aldrich SAB2701683, 2/1000) in buffer (1% sheep serum ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Female rats were administered an intraperitoneal injection of “Hellabrunner Mischung” comprising ketamine hydrochloride/xylazine hydrochloride solution (1ml/kg, Sigma Aldrich, Taufkirchen, Germany). The surgical procedure followed the description of Spicer and colleagues (44) ...
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bioRxiv - Neuroscience 2020Quote: ... Eluted proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting: hDAT and DAT/C-S were detected with amino-directed rat anti-DAT (MAB369, Millipore, 1:2000), and N-S/DAT and S/DAT/S were detected with amino-directed mouse anti-hSERT (MAb Technologies,1:2000) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
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bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS-T (0.05% Tween 20) and subsequently probed with the rat anti-HA mAb 3F10 (Sigma, 1:1,000 dilution), followed by biotin-conjugated anti-rat antibody (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Physiology 2020Quote: DN was induced experimentally in 12-h fasting rats except for the normal group through a single intraperitoneal injection of 0.5 mL STZ (Sigma-Aldrich, Deisenhofen, Germany) at a dosage of 60 mg/kg of body weight ...
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bioRxiv - Neuroscience 2021Quote: ... (2) rats underwent UVN and received intraperitoneally one L-thyroxine (L-T4) injection (10 µg/kg, dissolved in saline, Sigma, Cat#T2501) at the end of the surgery and one injection/day during the first 3 days post-lesion (UVN-T4 group ...
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bioRxiv - Cell Biology 2021Quote: ... For KIF18A localization analyses the following primary antibodies were used at the indicated dilutions: rat anti-α-tubulin 1:500 (MAB1864; Sigma Aldrich), rabbit anti-KIF18A 1:100 (A301-080A ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Pathology 2024Quote: ... The diabetic rats in the DM+GR group were given a standard diet and were also given glyburide (Sigma-Aldrich; Merk KGaA) at 4 mg/kg in 0.5 ml of 0.5% Tween 80 solution as a supplement ...
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bioRxiv - Molecular Biology 2023Quote: ... Rabbit anti-TgTom40 (van Dooren et al., 2016, 1:2000 dilution) and rat anti-HA high affinity (Sigma, 11867431001, 1:200 dilution) primary antibodies were used ...
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bioRxiv - Physiology 2023Quote: ... FGF21 was measured by using a commercially available enzyme-linked immunosorbent assay kit (rat/mouse FGF21 enzyme-linked immunosorbent assay kit; Millipore, Billerica, MA), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher], and anti-NeuN [Millipore Sigma] ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and subsequently incubated with anti-CD16/CD32 (eBioscience, Cat #14-0161-86, clone 93) and 10% rat-IgG1 (Sigma-Aldrich, Cat #I8015) in PBS containing 0.1% BSA (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... incubator with daily medium changes 96-well plates (Falcon: 353219) precoated with Type 1 collagen solution from rat tail (Sigma Aldrich: C3867).
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bioRxiv - Cell Biology 2020Quote: ... pDEST15 plasmids were transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore). Bacteria cultures were grown with the proper antibiotic at 37°C until an OD of 0.6 was obtained ...
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bioRxiv - Genetics 2021Quote: ... coli strains DH5α was used for plasmid maintenance and SM10 (Novagen, Hornsby Westfield, Australia) HB101 (pRK2103 ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μg of plasmid were mixed with 60 μl of CaCl2 2.5 M (Sigma) and endotoxin-free water up to 500 μl ...
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bioRxiv - Genomics 2020Quote: ... Endotoxins were removed from the plasmids using the Endotoxin removal Solution (Sigma, E4274-25ML).
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bioRxiv - Microbiology 2019Quote: Chimeric phage was generated by homologous recombination using plasmid pNL9164 (Sigma-Aldrich, MO, USA) in S ...
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bioRxiv - Synthetic Biology 2020Quote: The creation of all bacterial IgG expression constructs involved plasmid pCOLADuet™-1 (Novagen), which is designed for the coexpression of two target genes from independent upstream T7 promoter/lac operator regions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Variants were subcloned from the pEDA3 background into protein expression plasmid pET-29b(+) (Novagen) or into the constitutive expression plasmid pEDA2 (Wrenbeck et al ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant plasmid DNA was transferred to Escherichia coli strain Rosetta 2(DE3)pLysS (Novagen). At least four colonies for each experiment were sequenced to check for errors in the PCR ...
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bioRxiv - Immunology 2019Quote: ... The purified plasmid was transformed into S2 cells grown in EX-CELL 420 (Sigma) with 25 μL ExpreS2 Insect-TR 5X (ExpreS2ion Biotechnologies) ...
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bioRxiv - Biochemistry 2019Quote: ... The purified plasmid was transformed into S2 cells grown in EX-CELL 420 (Sigma) with 25 μL ExpreS2 Insect-TR 5X (ExpreS2ion Biotechnologies) ...
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bioRxiv - Microbiology 2019Quote: ... strains harbouring pGCP123 plasmids were grown in 1000 µg/mL kanamycin (Sigma Aldrich, USA) and strains harbouring pMSP3535 plasmids were grown in 100 µg/mL erythromycin unless stated otherwise ...
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bioRxiv - Genetics 2021Quote: The Csm2-Psy3 heterodimer was cloned into the dual expression plasmid pRSFDuet (EMD Millipore) which encode a Strep-tagged Csm2 and FLAG-tagged Psy3 ...
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bioRxiv - Microbiology 2021Quote: ... and 0.8 µg plasmid expressing HIV-1 gag-pol using X-tremeGENE 9 (Sigma). Media was changed 6 h later and lentivirus containing culture supernatants were collected at 48 and 72 h post-transfection ...
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bioRxiv - Biochemistry 2020Quote: The phytochrome from Deinococcus radiodurans strain R1 (DrBphP, gene DR_A0050) in pET21b(+) plasmid (Novagen) was kind gift from Prof ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid expression was induced with 1 mM IPTG (Sigma-Aldrich, St. Louis, MO, USA) and the culture was grown further at 16 °C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... Bacteria were grown overnight with the plasmid of interest in Lysogeny Broth (Sigma-Aldrich) and the respective antibiotics ...
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bioRxiv - Biochemistry 2021Quote: ... a high-copy plasmid from a different incompatibility group (pBR223 origin of replication; Novagen) containing the lacIq repressor ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting PCR fragment and the Plasmid DNA of the overexpression vector pET28a(+) (Novagen) were digested with NdeI and BamHI ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... strains harbouring pMSP3535 plasmids were selected using 100 ug/mL erythromycin (Sigma Aldrich, USA) and induction of gene expression was performed using 5 ug/mL nisin from Lactococcus lactis (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... the construct plasmid was transformed into BL21(DE3)pLysE bacteria (Sigma-Aldrich, CMC0015-20X40UL), and grown in TB supplemented with 100 µg/mL ampicillin and 33 µg/mL chloramphenicol at 37 °C until an OD600 of 0.4 was reached ...
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bioRxiv - Microbiology 2022Quote: ... 25 µg of viral plasmid and 100 µl branched PEI (pH 7.4) (Sigma-Aldrich) were added to the cell culture ...
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bioRxiv - Biochemistry 2022Quote: ... The expression plasmid was transformed into Rosetta-Gami®2 (DE3) competent cells (Novagen) to maintain disulfide bond formation ...