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Citations for Millipore Sigma :
1951 - 2000 of 2589 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Mouse phospho-MLKL (Ser345) rabbit monoclonal (D6E3G) (Cell Signalling Technology) MLKL rat monoclonal 3H1 (available from Millipore MABC604; generated in-house) ß-actin mouse monoclonal AC-15 (Sigma A-1978)
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Neuroscience 2022Quote: ... Dissociated cortical cells from E18 Sprague Dawley rat pups were plated on slips or plates coated with poly-D-lysine (Sigma, MO). For live imaging ...
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bioRxiv - Bioengineering 2022Quote: ... the top well of m-μsims was coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Bioengineering 2022Quote: ... 35,000 cell/cm2 were added to chambers coated with a mixture of rat tail type I collagen (25 µg/cm2, Sigma Aldrich) and human fibronectin (5 µg/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6, G5555, Sigma-Aldrich; 1:1000 Rabbit anti-Cux1 ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from zebrafish and cultured rat brainstem and spinal cord neurons following the TRI Reagent isolation protocol (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Cell Biology 2019Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... electroporated retinas were placed on a 35-mm glass bottom dish (Pelco, Wilco Wells) and incorporated in collagen prepared as follow: 100 mg of rat tail collagen type VII (Sigma-Aldrich) was dissolved in 6ml of 16.7 mM acetic acid overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: ... Membranes were then incubated with the corresponding secondary antibodies (rabbit anti-mouse IgG/HRP, Dako, #P0260 and goat anti-rat IgG/HRP, Sigma, #A9037) at a 1:5000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... anti- Spz (rat, 1:50, requested from Satoshi Goto), Anti-Elav (rabbit, 1:50, DSHB) and Caspase 3 (rabbit, 1:500, Sigma Aldrich). Samples were washed four times for 10 minutes each in PBST and incubated at room temperature in secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated cortical cells from E18 Sprague Dawley rat pups were plated on slips or plates coated with poly-D-lysine (Sigma, MO). For live imaging ...
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bioRxiv - Neuroscience 2019Quote: ... Analysis by Western blot shows a band at 19 kDA from rat olfactory bulb which is blocked by inhibition with specific OMP immunizing peptide (Sigma datasheet).
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bioRxiv - Genetics 2020Quote: ... McA-RH7777 were cultured on collagen-coated dishes (from AGC Techno Glass, or self-prepared using 10 μg/cm2 of rat type I collagen from Sigma-Aldrich). Passages were performed when cells were semi-confluent ...
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bioRxiv - Physiology 2021Quote: ... extra blood was collected at each time-point and later plasma was separated by centrifugation (15min at 2,000 RPM) and stored at −80 °C for insulin measurement using a Rat/Mouse Insulin Elisa kit (EMD Millipore - EZRMI13K). Prior to sacrifice ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were coated with collagen type I at diluted 1:100 ratio with PBS (solution from rat tail) (Sigma-Aldrich, # C3867) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-Syt (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... FITC-labelled peptide samples (Li, i+4L, Li, i+4D or the RCM control) were dissolved in 100% rat serum (Sigma Aldrich) at an initial concentration of 4 μM ...
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bioRxiv - Neuroscience 2020Quote: A subset of male Long-Evans rats received intraperitoneal injections of 1.0 mg/kg lipopolysaccharide (LPS) from Escherichia coli (Sigma-Aldrich, L2630) in sterile saline (0.9% ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were incubated in 1:30 rat anti-DN-cadherin (DN-EX #8, Developmental Studies Hybridoma Bank) and 1:500 rabbit anti-GABA (A2052, Sigma-Aldrich) or 1:200 rabbit anti-Lucifer yellow (A5750 ...
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bioRxiv - Neuroscience 2022Quote: ... Micro-islands were generated as described previously (Meijer et al., 2012) by plating 8000/well rat glia on UV-sterilized agarose (Type II-A; Sigma, A9918) coated etched glass coverslips stamped with a mixture of 0.1 mg/ml poly-D-lysine (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Albumin and Lipocalin-2/NGAL values were obtained using MILLIPLEX MAP Rat Kidney Toxicity Magnetic Bead Panel 2 (Millipore Sigma, USA) with 1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: Rats received either a 30-minute infusion of corticosterone (0.75 mg/mL Corticosterone-2-hydroxypropyl-β-cyclodextrin; Sigma-Aldrich, Gillingham, UK) dissolved in sterile 0.45% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Pathology 2024Quote: The content of human IgG was quantitated both in the sera and aortic (tissue) homogenates from rats treated with chP3R99 or hR3 mAbs by a commercial ELISA kit (Sigma-Aldrich). Similarly ...
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bioRxiv - Microbiology 2024Quote: The experimental plate for HepG2/C3A cells was prepared by coating with 100 µg/mL of rat tail collagen (Sigma-Aldrich) and 0.02 M glacial acetic acid in PBS for a minimum of 2h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was started after 5 min preincubation by adding 13 µL of a microsome mix from the liver of Sprague-Dawley rats (Sigma Aldrich, 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Neuroscience 2023Quote: ... thoracic and lumbar dorsal root ganglia neurons were extracted from E17.5-E18.5 rat embryos and plated on glass coverslip coated with poly-L-ornithine (Sigma-Aldrich, MO, USA) for 4-6 hours before experiments.
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bioRxiv - Cell Biology 2023Quote: ... each coverslip was washed 3 times in PBS then placed on top of 50 µL drops of the secondary antibody mixture of goat anti-rat TRITC (1:100; Millipore Sigma) and goat anti-mouse FITC (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Neuroscience 2023Quote: ... After 1-2 months old rats undergo trans cardiac perfusion with PBSx1 (02-023-5A, Biological Industries) followed by neutral buffered 10% formalin (HT5011, Sigma-Aldrich). The whole brain was removed and immersed in 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were incubated overnight at 4°C with gentle shaking with rat anti-DAT (1:1000, Millipore Cat# MAB369, RRID:AB_2190413), rabbit anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were dissected from postnatal Sprague Dawley rats (P0-P2) and dissociated with 0.25% trypsin (Sigma Aldrich, Merck, Darmstadt, Germany) and 0.004% DNAse (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: Decellularized rat lung and isolated lobes were perfused with a solution of 0.1% Methylene blue solution (Cat. # 03978, Sigma Aldrich, UK) in PBS either from the trachea or pulmonary artery ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse and rat kidneys were fixed by perfusion with 50-60 mL of 4% paraformaldehyde in PBS (158127, Sigma-Aldrich), dehydrated and embedded in paraffin at 58C ...
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bioRxiv - Physiology 2023Quote: ... the supernatant and the fasting and 15 min GTT serum was performed using Rat/Mouse Insulin ELISA kit (EZRMI-13K, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... for 30 min at room temperature and then incubated overnight at 4°C with anti-HA (rat, 1:1000 dilution, Sigma-Aldrich) antibody in TBS containing 10% goat serum ...
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bioRxiv - Immunology 2023Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 10 dpi and ending at 34 dpi.
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Cell Biology 2023Quote: ... and of PTMs (tyrosinated tubulin: YL ½ antibody, ab6160; rat monoclonal, Abcam; acetylated tubulin: T6793 antibody, clone 6-11B-1, mouse, Sigma-Aldrich; polyglutamylated tubulin ...
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bioRxiv - Neuroscience 2024Quote: ... A rat anti-D1DR monoclonal antibody was used for D1DR-receptor staining (1:500; Sigma-Aldrich, St Louis, MO; Catalog #D2944). To enhance the GFP signals ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti- cytokeratin 8 (TROMA-I; Developmental Studies Hybridoma Bank; 1:500) and rabbit anti- Sox9 (Millipore, Burlington, MA; 1:750). Detection of antibody binding was with the appropriate species-specific Biotin-SP-conjugated goat anti-antibodies (1:400 ...
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bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then blocked in 3% BSA in PBS containing 0.2% Tween 20 before staining with rat anti-HA mAb 3F10 (Sigma, diluted 1:1,000) primary antibody in blocking buffer ...