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Citations for Millipore Sigma :
2251 - 2300 of 2589 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI) (Sigma) with a mass ratio of 1:4 ...
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bioRxiv - Biochemistry 2022Quote: ... The EC1-2 construct was cloned into pET21a plasmid system and transformed into BL21 (DE3) competent cells (Novagen). Cultures were grown in autoinduction media (PMID ...
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bioRxiv - Biophysics 2022Quote: ... The EC1-2 construct was cloned into pET21a plasmid system and transformed into BL21 (DE3) competent cells (Novagen). Cultures were grown in autoinduction media 24 overnight and harvested via centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... Parasites containing the episomal plasmids selected with WR99210 were subsequently grown with 400 μg/ml Neomycin/G418 (Sigma) to select for integrants carrying the desired genomic modification as described previously (Birnbaum et al. ...
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bioRxiv - Immunology 2021Quote: ... calcium-phosphate-DNA-particle were generated by mixing the plasmid DNA’s with a 250 mM CaCl (Sigma-Aldrich) solution which was then added dropwise to an equal volume of 2 x hepes buffered saline and subsequently incubated for 20 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... MDCK cells were transfected with linearized plasmid DNA and selected with either 500 μg/mL G418 (Sigma-Aldrich) or 400 μg/mL Zeocin (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... TRCN0000028509 (sh-AC2) and the scramble control pLKO.1-Puro plasmid (sh-scr) were obtained from Sigma-Aldrich. The sequence of shRNAs are:
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bioRxiv - Neuroscience 2020Quote: ... CGNs (3 × 106 cells) and plasmids were suspended in 70 μl of Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) and exposed to decay pulses using a CUY21-edit II pulse generator (BEX ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... cells that had integrated plasmid were selected in media supplemented with G418 (400 µg mL-1 Sigma-Aldrich) for 10 days ...
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bioRxiv - Microbiology 2020Quote: ... GBS bearing the pFfluc-Erm plasmid must be exposed to the cofactor D-luciferin (Millipore Sigma cat. # L9504), which was prepared in a filter-sterilized stock solution at a concentration of 28 mg/mL in water ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids encoding CaMKII variants were co-transformed with lambda phosphatase into Rosetta 2(DE3)pLysS competent cells (Millipore). Expression was induced overnight with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2020Quote: ... and cargo in a 1:4:1 ratio with PEI reagent (1:3 plasmid to PEI ratio, Sigma). Three days after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... under a stereoscope and using a glass microcapillary 1-2μl of plasmid DNAs was injected together with Fast Green (0.1%, Sigma) into different ventricles of the organoids ...
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bioRxiv - Biochemistry 2019Quote: ... the corresponding genes were amplified by PCR from S.cerevisiae genome and cloned into the plasmid pETDute-1 (Novagen). To construct N-terminal 6×His tagged Ups1-Mdm35 fusion protein ...
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bioRxiv - Cancer Biology 2020Quote: The puromycin selection gene was replaced with green fluorescent protein (eGFP) cDNA in Lentiviral pLKO.1 plasmid (Sigma). shRNA oligos (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... parasites carrying the WR99210-selected episomal plasmid were cultivated in the presence of 400 µg/ml G418 (Sigma) to select for integrants with the desired modification of the genomic locus ...
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bioRxiv - Molecular Biology 2021Quote: ... Taq pol and StuI genes were subcloned for expression in the pET24-d plasmid (Novagen, Merck Millipore, Germany) using its NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Taq pol and StuI genes were subcloned for expression in the pET24-d plasmid (Novagen, Merck Millipore, Germany) using its NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... see Table 1 for complete plasmid list) was induced by treating cells with 2 µg/mL doxycycline (Sigma) for at least 72 hours ...
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bioRxiv - Cell Biology 2021Quote: ... transformed with pET:THMT:PTBP3 (empty plasmid from Peti and Page, 2007) at 15°C overnight with 400 μM IPTG (Sigma). Induced pellets were resuspended in low imidazole purification buffer [20 mM sodium phosphate (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: Plasmids for knockdown of CCL2 (TRCN0000301701 and TRCN0000301702) and CCL7 (TRCN0000317599, TRCN0000068135, and TRCN0000068136) were obtained from Sigma. shRNA control plasmid (SHC002 ...
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bioRxiv - Microbiology 2022Quote: Plasmids containing WT or mutant NS1 sequence were transfected into FreeStyle 293F cells using polyethylenimine (PEI) (40K) (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The CAG-tdtomato or CAG-tdtomato-T2A-RHEBCA plasmid (3.5μg/μl) was mixed with the dye Fast Green (0.3 mg/ml,Sigma-Aldrich) and injected (~1 μl/embryo ...
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bioRxiv - Molecular Biology 2024Quote: The stable cas9 expressing Hep3B (Hep3Bcas9) cell line was generated via transfection with Cas9 plasmid (Sigma catalog #CAS9HYGROVP) at a ratio of 6:1 using lipofectamine (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... incubating 500 ng of supercoiled plasmid DNA (pcDNA5 FRT/TO and pOG44) with 100 µM Mirin (Sigma-Aldrich), 3.4 µM doxorubicin (known to exhibit strong DNA intercalation ...
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bioRxiv - Cell Biology 2023Quote: ... All constructs were prepared for transfection using either the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich) or the NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL) ...
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bioRxiv - Cell Biology 2023Quote: ... All constructs were prepared for transfection using either the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich) or NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL).
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bioRxiv - Microbiology 2023Quote: ... the plasmid pBSU101- GFP was maintained with the addition of 120 μg/ml of the antibiotic Spectinomycin (Sigma) in the plates as well as growth media.
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bioRxiv - Microbiology 2023Quote: ... Strains with the PCdrA∷gfp and pMH487 plasmids were grown with 60 μg/mL Gentamycin (Sigma-Aldrich, G1914) for plasmid selection ...
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bioRxiv - Microbiology 2023Quote: ... All pCC2Fos replicons were extracted using the GenEluteTM HP Plasmid Miniprep Kit (Sigma Aldrich, St. Louis, Missouri, USA) and NucleoBond® Xtra Midi Plus (MACHEREY-NAGEL ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich) along with 2nd generation lentiviral packaging and envelope plasmids ...
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bioRxiv - Microbiology 2023Quote: ... We used the PR8 pDZ-HA plasmid as a template and Kod Hot start DNA polymerase (Millipore Sigma). We followed the manufacturer’s recommendations to PCR mutagenize the pDZ-HA open reading frame ...
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bioRxiv - Microbiology 2023Quote: ... We used the pH28D14 HC and LC plasmids as templates and Kod Hot start DNA polymerase (Millipore Sigma). We followed the manufacturer’s recommendations to PCR mutagenize the VH and VL gene segments ...
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bioRxiv - Cell Biology 2023Quote: ... after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich, Merck). To generate pARL-PfCen1-GFP ...
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bioRxiv - Biochemistry 2023Quote: ... of p150 from rubella virus strain RVi/Brooklyn.NY.USA/98/1B CRS (GenBank JN635282; UniProt G3M8F4) was cloned into the pET24a plasmid (Novagen) with a C-terminal hexahistidine purification (His6 ...
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bioRxiv - Bioengineering 2023Quote: To construct the plasmids harboring the retron arrays in their different architectures the pCDF-DUET-1 vector (Novagen) was used as a backbone ...
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Microbiology 2023Quote: ... Transformants containing the plasmid were selected on tryptone soy agar (TSA) containing 10 μg/mL chloramphenicol (Cam, Sigma) and incubated for 24-48 h at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... the full NSm ORF was amplified from the pT7riboBUNM plasmid with KOD Hot Start Master Mix (EMD Millipore) using the primers listed in Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... All plasmids were checked by Sanger sequencing (Microsynth) prior to cloning into a modified pET28b-SUMO vector (Novagen). The recombinant plasmids include an N-terminal 8xHis-tag followed by a small ubiquitin-like modifier (SUMO ...
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bioRxiv - Cell Biology 2022Quote: ... incubated at room temperature with secondary antibodies coupled to horseradish peroxidase (1:20,000 Thermo Fisher goat-anti-rabbit, or 1:20,000 Millipore Sigma goat-anti-rat in 5% nonfat dry milk). Membranes were washed again in PBS containing 0.1% Tween 4 x 15 min ...
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bioRxiv - Biophysics 2021Quote: ... The plasmids were incorporated into pcDNA3.1(+) vectors and were transiently transfected into HEK 293T cells using PEI (Milipore Sigma) as previously described 66 ...
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bioRxiv - Microbiology 2021Quote: ... and oriT from pUC18T-mini-Tn7T-Tp-dsRedExpress were PCR amplified and introduced into a linearized pYES1L plasmid (Novagen) using the Gibson assembly method 46 (Supplementary Fig ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fluorescent proteins encoded on the plasmid also carries a gene for antibiotic resistance (Gentamicin 418, Sigma-Aldrich: G418). Cells were cultured in autoclaved HL5 medium (per L ...
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bioRxiv - Molecular Biology 2020Quote: ... U2OS cells were first transfected with the pCMV-Tet3G plasmid and selected using 250 µg/ml G418 (Sigma-Aldrich). The G418-resistant clone was maintained as the Tet3G-expressing U2OS cell line ...
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bioRxiv - Microbiology 2019Quote: ... a source of T7 RNA Polymerase was provided by co-transforming another plasmid pAR1219 (Sigma-Aldrich, St. Louis, MO). The plasmids pACD4K-C::emtA and pAR1219 were maintained by selection on chloramphenicol and ampicillin ...
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bioRxiv - Microbiology 2019Quote: ... All media was supplemented with 50μg/ml kanamycin to select for plasmid maintenance and 0.1% of Tween-20 (Polysorbate-20, Sigma-Aldrich) to reduce sticking of cells to the sides of microfluidic devices ...
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bioRxiv - Molecular Biology 2019Quote: ... and the plasmid encoding lentiviral genome with PGK-puroR (pLKO.1) alone or additionally with CMV-TurboGFP (SHC003, Sigma). Virus containing media was collected on days 2 and 3 after transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... and a mouth pipette were used to inject into the caudal neural tube the electroporation mix (1 µg/µl each plasmid in injection solution: high viscosity carboxymethylcellulose 0.33% (Sigma); Fast Green 1% (Sigma) ...