Labshake search
Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Cells were grown to a density of 1 × 106 cells per mL and treated with 10 µM ouabain (Sigma-Aldrich) or with 1 µM STS (Sigma-Aldrich ...
-
bioRxiv - Genomics 2019Quote: ... Cells were then incubated with a STAT3 antibody (9139, Cell Signalling) in blocking buffer + 0.1% tween-20 (P1379, Sigma Aldrich) for 1-2 hours at room temperature or overnight at 4°C on a shaker ...
-
bioRxiv - Biophysics 2020Quote: African green monkey kidney fibroblast-like cells (COS7, Cell Lines Service GmbH, Eppelheim, #605470) were cultured in DMEM (Sigma, #D8062) containing 10 % FCS (Sigma-Aldrich ...
-
bioRxiv - Genomics 2021Quote: ... IMR-90 cells were cultured in cell media supplemented with 1 mM or 2.5 mM DMOG (Sigma-Aldrich, cat.no. D3695) for 12 hours before PFA fixation.
-
bioRxiv - Cell Biology 2020Quote: ... centrifuging to remove the medium and suspending the pelleted cells at 5 × 106 cells/mL in a solution containing 20 g/L bovine serum albumin (Sigma), 0.2% (v/v ...
-
bioRxiv - Neuroscience 2020Quote: ... The phenotype of IdU-IR cells and CldU-IR cells was determined using rabbit anti-calbindin antibodies (1/200, Millipore) that were revealed with Alexa 488-goat anti-rabbit IgG antibodies (1/500 ...
-
bioRxiv - Genomics 2021Quote: Protein extracts were prepared by lysing germ cells isolated as describe above (RNA-seq section) or K562 cells in RIPA Lysis buffer (Millipore) containing 50 mM Tris–HCl ...
-
bioRxiv - Immunology 2021Quote: ... Monolayers were then rinsed thoroughly with Promocell DC generation medium to remove non-adherent cells and subsequently were cultured for 6 days in dendritic cell generation medium supplemented with cytokines (Sigma) to generate immature MoDCs.
-
bioRxiv - Immunology 2021Quote: Bone Marrow cells were isolated as described above and 107 cells were resuspended in 10 mL of RPMI-1640 (Sigma) supplemented with FBS (10% v/v) ...
-
bioRxiv - Immunology 2021Quote: ... and SP-R210(KO) RAW 264.7 cells were detached using non-enzymatic cell dissociation medium (Sigma-Aldrich Cat#C1544-100ML) and washed in PBS containing 2% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2020Quote: HeLa or HEK293T cells were grown on coverslips in the 24-well plate and HEK293T cells were pre-coated with PLL (Sigma) in a 24-well plate before transcription and treatment ...
-
bioRxiv - Biophysics 2020Quote: ... Dead cells were removed on day 6 after T-cell isolation by means of density-dependent gradient centrifugation (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were trypsinazed and plated at a density of 25000 cells/ml in 6-multiwell plates in DMEM (Sigma, UK), supplemented with 10% fetal bovine serum (Sigma ...
-
bioRxiv - Biochemistry 2020Quote: ... ALMC-2 or KAS-6/1 plasma cells were plated 100,000 cells/well in 150 μL of media in 96-well MultiScreenHTS filtration plates (EMD Millipore). Five replicates were treated with DMSO or compounds at the indicated concentrations and incubated for 18 hr ...
-
bioRxiv - Microbiology 2021Quote: ... Briefly a T175 flask of KC cells in suspension of growth media (Schneider’s insect cell media (Sigma-Aldrich, Dorset, UK); 10% Gibco® heat-inactivated fetal calf serum (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... The next day cells were washed three times with PBS and cultured with DMEM (DU145 cells) or RPMI (LNCaP cells) medium supplemented with 2 mM [U-13C5] L-glutamine (Sigma) for 10 ...
-
bioRxiv - Immunology 2020Quote: A total of approximately 1×106 splenocytes with WT Treg cells 1:1 mixed with KO Treg cells were adoptively transferred into cyclophosphamide (CTX, Sigma) treated (single dose at 200mg/kg ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
-
bioRxiv - Cancer Biology 2020Quote: ... Red blood cells (RBC) in the cell pellet were lysed with RBC lysis buffer [155 mM NH4Cl (11209, Sigma-Aldrich), 10 mM KHCO3 (2940-01 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
-
bioRxiv - Cell Biology 2021Quote: ... The PKH26:MYH10-KD co-cultures 3T3-L1 cells were labeled with 0.5λ PKH26 Fluorescent Cell Linker Kit (Sigma-Aldrich); the GFP cells were added after one day.
-
bioRxiv - Cell Biology 2021Quote: ... HeLa cells were then transduced with the filtered supernatant and the cells were selected with 1 μg/ml puromycin (Millipore). For the rescue assay ...
-
bioRxiv - Biochemistry 2021Quote: ... SP cells lacking endogenous mRNA were resuspended (3×106 SP cells/mL as determined by trypan blue (Sigma-Aldrich, T8154) staining ...
-
bioRxiv - Biochemistry 2020Quote: Human embryonic kidney (HEK) 293 cells were purchased from ATCC and HEK-293 PDE4A4 stable cell line established by Millipore. HEK-293 and HEK-293 PDE4A4 cells were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and the cell pellets were chemically lysed using 0.4 mL lysis buffer (CelLytic B cell lysis reagent (Sigma-Aldrich #B7310), 0.1 mg lysozyme ...
-
bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
-
bioRxiv - Microbiology 2021Quote: GFP-LC3 HeLa cells or HeLa-Difluo hLC3 cells were seeded onto Nunc Lab-Tek Chamber Slide system 8 wells (Sigma). The day of the experiment ...
-
bioRxiv - Plant Biology 2020Quote: ... A variety of approaches to degrade the suberin-lignin-containing bundle sheath cell walls with the addition of other enzymes failed to produce healthy cells: Laccase (Sigma) and manganese peroxidase (Sigma) ...
-
bioRxiv - Microbiology 2020Quote: Vero cells and VeroE6 cells expressing TMPRSS2 (VeroE6/TMPRSS2) were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma-Aldrich, USA) supplemented with 5% fetal bovine serum (Gibco-BRL ...
-
bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
-
bioRxiv - Cell Biology 2021Quote: ... Analysis of mRuby-PKR localization during the cell cycle was performed after synchronizing cells in G1 with thymidine (Sigma-Aldrich). Briefly ...
-
bioRxiv - Cancer Biology 2020Quote: 50,000 cells (cell lines) or 250,000 cells (spike-in and patients samples) were applied onto poly-L-lysine hydrobromide (PLL) (Sigma Aldrich) -coated microscope cover glasses (24×60 mm ...
-
bioRxiv - Cell Biology 2021Quote: ... iPSC-CMs were frozen in batches (>5 million cells) in CryoStor media (1 million cells/100 μLs CryoStor; Millipore Sigma) and placed in liquid nitrogen for disease modelling experiments ...
-
bioRxiv - Biophysics 2020Quote: ... followed by 99 μL of TZM-bl cells at 0.1 million cells/ml containing 10 μg/ml DEAE-Dextran (Sigma Aldrich). Final concentrations of the small molecules were 90 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with PBS and 1×106 fixed cells were permeabilized with 0.1 % Triton X-100 (Sigma-Aldrich) in PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... and CH-5high/CH-6high subpopulation breast cancer cells (cell numbers: 0.5million) were mixed with 100μl Matrigel/cell culture media mixture (Matrigel: cell culture media = 1:2) (Sigma or Corning). Indicated cancer cells/Matrigel/ cell culture media mixtures were injected into NOD/SCID female for breast cancer xenograft (the Jackson Laboratory ...
-
bioRxiv - Developmental Biology 2022Quote: mESC carriers were maintained in Feeder Cell medium in Corning cell culture dishes precoated with 0.1% Gelatin (ES-006-B, Sigma-Aldrich), at 37 □ suppled with 5% CO2 at one atmospheric pressure ...
-
bioRxiv - Genomics 2022Quote: ... The cells investigated here are maintained by the European Collection of Cell Cultures (ECACC) and were purchased from Sigma-Aldrich, or obtained from the International Histocompatibility Working Group (IHWG ...
-
bioRxiv - Immunology 2022Quote: ... For cell sorting cell suspensions were stained for viability with 1:3000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) immediately before sorting.
-
bioRxiv - Neuroscience 2022Quote: ... High Five™ insect cells for the expression of PP2A A/C complex were cultured in EX-CELL 405 Serum-Free medium (Millipore-Sigma) at 27°C with 130 rpm ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were maintained in the cell culture medium containing Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% (v/v ...
-
bioRxiv - Cancer Biology 2022Quote: ... and CH-5high/CH-6high subpopulation breast cancer cells (cell numbers: 0.5million) were mixed with 100μl Matrigel/cell culture media mixture (Matrigel: cell culture media = 1:2) (Sigma or Corning). Indicated cancer cells/Matrigel/ cell culture media mixtures were injected into NOD/SCID female for breast cancer xenograft (the Jackson Laboratory ...
-
bioRxiv - Biochemistry 2022Quote: ... All other cell lines were purchased from ECACC (European Collection of Authenticated Cell Cultures) through a local distributor (Millipore-Sigma). Adherent cells were grown to half dish confluence ...
-
bioRxiv - Biochemistry 2022Quote: ... All other cell lines were purchased from ECACC (European Collection of Authenticated Cell Cultures) through a local distributor (Millipore-Sigma). Adherent cells were grown to half dish confluence ...
-
bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... cell de-adhesion dynamics was quantified by performing live cell time lapse imaging following treatment with 0.5mM EDTA (Sigma-Aldrich). For the experiment ...
-
bioRxiv - Neuroscience 2021Quote: ... and iNs (converted in T25 flasks starting from 250,000 fibroblasts/ sample or converted in Nunc Delta treated 6-well plates starting from 200,000 plated fibroblasts/ sample) were harvested as follows: the cell medium was removed and the cells were lysed in RIPA buffer (Sigma) with 4% protease inhibitor cocktail (PIC ...
-
bioRxiv - Immunology 2020Quote: ... Fam72a+/+ CH12 cells (20 × 104 cells/mL) were cultured 20 hours with 10uM of CDK1/cyclin B1 inhibitor RO-3306 (Sigma), followed by exchange into inhibitor-free culture media ...
-
bioRxiv - Molecular Biology 2020Quote: pCDNA3 vectors expressing HA-tagged full size LARP6 or La-domain were transfected into HEK293 cells and 24h after transfection the cells were fixed and incubated with anti-HA primary antibody (Sigma), followed by incubation with fluorescent secondary antibody ...
-
bioRxiv - Neuroscience 2021Quote: ... Whole lumbar spinal cords were dissected from C57Bl6 mice and incubated with 1.25% collagenase in endothelial cell media (M199 media, 60mg/ml endothelial cell growth supplement and 50ug/ml Heparin; Sigma-Aldrich) for 30 minutes at 37°C ...