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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Effector (TILs) and target cells (autologous tumor cell lines) were labeled respectively with 1μM PKH67 and PKH26 fluorescent dyes (Sigma-Aldrich) according to the manufacturer’s protocol and loaded on the chip array ...
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bioRxiv - Immunology 2022Quote: ... entire viral medium was added to 4×106 HL-60 cells (seeded at a density of 0.25×106 cells/mL) in presence of 10 µg/mL polybrene (Sigma; TR1003). After 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Wells were washed twice with PBS to remove T cells and the number of remaining target cells was determined by culturing with 10 μg/mL resazurin (R7017, Sigma) and measuring fluorescence signal in a plate reader ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... About 2×106 cells of HEK293T cells were transfected with 2 ug of plasmids encoding S proteins using polyetherimide (PEI; Sigma). After 16 hours of incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 13 mm cell culture coverslips coated with 0.01 mg/ml poly-L-lysine hydrobromide (Sigma-Aldrich) at a density of 50,000 cells/cm2 ...
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bioRxiv - Systems Biology 2022Quote: ... Cell suspension samples were used for cell counting using Scepter™ 2.0 Automated Cell Counter with 60 μm sensors (Merck Millipore), for cell viability and cellular ATP assessments using CellTiter-Glo® Luminescent Cell Viability Assay (Promega ...
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bioRxiv - Systems Biology 2022Quote: ... The cellular suspension was centrifuged to yield a pellet and then depleted of erythrocytes by resuspending the cells in pre-chilled Red Blood Cell Lysis solution (Sigma). The spleen cells were washed twice in DMEM medium containing 25mM HEPES ...
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bioRxiv - Physiology 2022Quote: 200 μL of the ventricular cell suspension (40,000 per mL) were seeded on glass-bottom dishes (Cell Nest, 801001) coated with ECM gel (Sigma, E1270), and allowed to settle for 40 minutes ...
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bioRxiv - Genomics 2022Quote: ... cells were treated with 134 μM (i.e. the final concentration in cell culture) Sodium Arsenite (NaAsO2; Sigma-Aldrich, Cat # S7400), a heavy metal oxidative stressor ...
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bioRxiv - Cancer Biology 2022Quote: ... the fibrosarcoma cell line HT1080 and the lung cancer cell lines A549 were maintained in RPMI medium (Sigma-Aldrich, R8758) with 10% (v:v ...
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bioRxiv - Developmental Biology 2022Quote: ... 12-well plates at an approximate cell density of 40,000 cells/cm2 in cSFDM with 2 μM Retinoic acid (Sigma, #R2625). From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies ...
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bioRxiv - Cell Biology 2022Quote: NALM6 target cells were labelled with CellVue membrane dye (CellVue Claret Far Red Fluorescent Cell Linker Kit protocol; Sigma-Aldrich) and 6.25 × 104 or 1.25 × 105 cytotoxic CD8+ T cells were used at indicated effector to target rations ...
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bioRxiv - Systems Biology 2024Quote: ... Reporter cell lines (100-150 million cells) were transduced with packaged retrovirus in the presence of 6 µg/ml polybrene (Sigma) and at low transduction efficiency (< 20% ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates were prepared from approximately 3 OD600 units of cells using a trichloroacetic acid (TCA) (Sigma Aldrich, Cat#T9159) lysis method60 ...
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bioRxiv - Neuroscience 2024Quote: ... Sigma-Aldrich)-coated 6-well plates (200,000 cells/well) for western blot experiments and in 96-well plates (10,000 cells/well) for MTT assay (#M2003, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... Cell size and volume values were obtained using a Scepter™ Handheld Automated Cell Counter (EMD Millipore Corporation, Billerica, MA), which employs the Coulter principle of impedance-based particle detection.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Post incubation cells were washed twice more with PBS and detached with 5mL non-enzymatic cell dissociation solution (Sigma, UK). Cells were pelleted by centrifugation for 10 min at 1000xg ...
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bioRxiv - Microbiology 2024Quote: ... whole cell extracts were obtained by resuspending cell pellets in cell extract buffer (20 mM Tris [pH 7.0], 1× Protease Inhibitor Cocktail Set I (Sigma-Aldrich)) and transferred to sterile screw cap tubes containing silica beads ...
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bioRxiv - Immunology 2024Quote: ... red blood cell lysis was performed by adding 1mL of Red Blood Cell Lysing Buffer Hybri-Max™ (Sigma R7757) per spleen sample cell pellet ...
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bioRxiv - Cell Biology 2024Quote: ... were resuspended at cell densities of 8×106 cells/mL for each cell type into a hydrogel comprised of 6.5mg/mL fibrin (Sigma #341573) and supplemented with 0.2mg/mL fibronectin (Sigma #F1141 ...
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bioRxiv - Biophysics 2024Quote: The tsA-201 cells (a variant of human embryonic kidney HEK cells) were obtained from Sigma-Aldrich (St. Louis, MO). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... either 8x103 cells (for immunostaining) or 30x103 cells (for qPCR) were plated in 24-well plates with medium containing siRNAs (Sigma) and HiPerFect transfection reagent (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... gilal cell growth and proliferation was inhibited by treating the cell cultures with 50μM 5-fluoro-2-deoxyuridine (Sigma-Aldrich). Mature cultures were used at DIV 11-12 for experiments and contained more than 95% neurons.
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bioRxiv - Cell Biology 2024Quote: ... HEK293A cells were seeded onto dishes that had been coated with 10 µg ECL cell attachment matrix (Sigma 08-110) in DMEM or Stem Cell Qualified ECM gel (Sigma-Aldrich CC131 ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of dsRNAs were transfected into BmN4 cells (6 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma). dsRNAs were repeatedly transfected every 3 days for four times ...
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bioRxiv - Immunology 2024Quote: Activated TKO CD4+ T cells were activated with Cell stimulation cocktail plus protein transport inhibitors (eBioscience) and PMA (500 ng/ml, Sigma) for 4 hrs and then incubated with antibodies targeting cell surface markers ...
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bioRxiv - Biochemistry 2024Quote: ... Cell viability under 42 h compound treatment was determined via the Cell Counting Kit-8 (CCK-8, Sigma-Aldrich #96992) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... the HepG2 human hepatocyte carcinoma cell line and the HT29 human colon adenocarcinoma cell lines were purchased from the European Collection of Authenticated Cell Cultures (ECACC) through their distributor (Sigma). The HL1 cell line was purchased from Millipore ...
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bioRxiv - Cancer Biology 2024Quote: Cells were cell-surfaced stained and fixed overnight with 1% PFA prior to permeabilisation with 0.3% Saponin (Sigma-Aldrich Ltd) for 15mins at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Wells were washed twice with PBS to remove T cells and the number of remaining target cells was determined by culturing with 10 μg/mL resazurin (Sigma) for 2 hours and measuring fluorescence signal in a plate reader ...
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bioRxiv - Cancer Biology 2022Quote: For live cell imaging cells were seeded on glass bottom plates which were previously coated with either 1mg/ml fibronectin (CKPC cells) or 10 μg/ml laminin (COS-7 cells, #L2020, Sigma). The cells were imaged using a Zeiss 880 LSM with Airyscan microscope which has a 37°C humidified incubator and perfused with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: GM130 immunoprecipitates from SV589 cell lysate or N74-strep pulldowns from cell lysate were treated with 5-50 µg/ml RNase A (Sigma). The RNase inhibitor RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... hTERT RPE-1 cells or hTERT RPE-1 cells expressing wt or as Cdc7 were plated onto fibronectin (5μg/ml, Sigma-Aldrich) coated coverslips at approximately 30% confluence and synchronized with thymidine for 24 hours ...
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bioRxiv - Physiology 2023Quote: C2C12 myoblasts were seeded at the same cell density (1 × 105 cells/ml) on coverslips coated with laminin-1 (Sigma). 24 hours after seeding ...
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bioRxiv - Synthetic Biology 2022Quote: ... The mid log phase cells were diluted to 104 cells/ml (assuming 1 OD = 8×108 cells/ml) into LB with 0.005% Antifoam 204 (Sigma A6426) and 1 mg/ml BSA (Sigma-Aldrich A2153-100G) ...
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bioRxiv - Neuroscience 2022Quote: ... and plated on 35mm dishes (8×105 cortical cells and 4×105 hippocampal cells per dish) coated with poly-DL-ornithine (Sigma), in DMEM (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... EVs were obtained by incubating red blood cell with CaCl (5 mM) and other cell types with the calcium ionophore A23187 (1 mM, C9275, Sigma) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... After 24-hours BrdU was added to the media and 16h later cell proliferation was evaluated using the BrdU Cell Proliferation ELISA kit (EMD-Millipore). Colorimetric analysis was done with single channel 450nm intensity ...
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bioRxiv - Molecular Biology 2023Quote: ... Silencing KMT2B in Jurkat cells was conducted by transfecting the cells with 20nM of esi KMT2B (Sigma Cat No. EHU076961) using TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Neuroscience 2023Quote: ... WHA150446) with the ganglion cell layer side up and transferred turned upside down to a cell culture insert (Millipore, PICM0RG50) in a MatTek glass bottom dish (MatTek ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mL of a 2.0×10^5 cells/mL solution were added to single cell culture dishes (EP0030700112, Sigma-Aldrich) and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... SH-SY5Y cells and A549 cells were acquired from ATCC and maintained in high-glucose Dulbecco’s modified Eagle medium (DMEM; Sigma-Aldrich), supplemented with 10 % heat-inactivated foetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... We infected the GL261 cells by adding the lentivirus to the glioma cell culture medium with polybrene (optional, 10 μg/ml working concentration, Sigma) to increase the efficiency of infection ...
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bioRxiv - Molecular Biology 2023Quote: ... Target cells without effectors served as a negative control and target cells incubated with 2% Triton X-100 (Merck-Sigma) served as positive control (maximum killing).
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bioRxiv - Cell Biology 2022Quote: ... Differentiated HL-60 cells (dHL-60) were generated by incubating cells in media containing 1.57% Dimethyl Sulfoxide (DMSO, Sigma, #D2650). Here ...
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bioRxiv - Genetics 2022Quote: ... K562 cells were transduced at a cell concentration of 5×105 cells/ml in the culture medium supplemented with 4 μg/ml polybrene (Sigma). After 24 h ...
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bioRxiv - Immunology 2023Quote: HBEC3-KT cells or MLE-15 cells were suspended in NP-40 lysis buffer containing Halt protease and phosphatase inhibitor cocktail (Millipore), disrupted by sonication ...
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bioRxiv - Genomics 2023Quote: Two thousand cells of each cell line were plated in each well of Corning 96 well black microplates (Millipore Sigma) and treated with trametinib (Selleckchem ...
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bioRxiv - Immunology 2023Quote: Naïve spleens from PbTII and PbTII.nzEGFP mice were harvested and mashed through 70-100µm cell strainers and the red blood cells (RBCs) were lysed using RBC Lysing Buffer Hybri-Max (Sigma-Aldrich) or BD Pharm Lyse™ (BD) ...
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bioRxiv - Biochemistry 2023Quote: Cells were seeded onto an XF96 cell culture microplate coated with 0.001% (w/v) poly-L-lysin (Sigma-Aldrich P4707) at 30,000 cells/well and incubated for 48 hrs in glucose-based media supplemented with 0.25 μg/ml doxycycline ...