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Citations for Millipore Sigma :
2051 - 2100 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 150 μL of cell suspension was manually aspirated into the cell loading tip pre-filled with mineral oil (Sigma-Aldrich). Detailed fabrication protocol of cell delivery and droplet collection tips is provided in our recent article37.
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bioRxiv - Cancer Biology 2023Quote: ... Target cells were transduced sequentially two times and the transduced cell population was selected using 1 μg/ml puromycin (Sigma) as described previously 15.
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bioRxiv - Immunology 2023Quote: ... Erythrocytes were removed by incubating the cell pellet with red blood cell lysis buffer (Sigma-Aldrich, R7757; St. Louis, MO) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Molecular Biology 2023Quote: ... During the incubation step the HeLa total cell lysate was prepared by lysing freshly collected HeLa cells in RIPA buffer (Sigma) (1 ml per 15 cm plate ...
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bioRxiv - Developmental Biology 2023Quote: C57BL/6 ES and C57BL/6-tdTomato+ ES cell lines were cultured on feeder cells with 2il medium: DMEM (Millipore) supplemented with 15% FBS (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... and HOS-ACE2/TMPRSS2 cells (HOS cells stably expressing human ACE2 and TMPRSS2)31,32 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500M ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were plated onto Agilent Seahorse XF96 cell culture plates or 12-well cell culture dishes coated with 0.1% gelatin (Sigma #G1393) in DMEM/F12 medium (Gibco #11330057 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were trypsinized and 30,000 cells/spheroid were plated onto Corning 96-well spheroid microplates (Cat. CLC4920, Millipore Sigma) for 24 hours for spheroid aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended as per manufacturer’s instructions for RNAse-rich cell lines using 2-MercaptoEthanol (Sigma-Aldrich, M6250-250 mL) and on-column DNAse digestion was performed to further purify the product (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Live-cell images where we tested the effect of the spatially restricted antagonist in HeLa cells were carried out by incubating the cells in 1 μM dobutamine (D0676, Sigma) at 37°C for 20 min before indicated spatially selective antagonists were added ...
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bioRxiv - Pathology 2023Quote: ... cells were seeded at a density of 120000 cells in plugs made of Rat tail collagen I (Millipore; 08-115) in a 24-well plate and treatment done with Control ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells harboring GFP-EBV BACmid cells were maintained complete DMEM supplemented with 1 mg/ml puromycin (Sigma-Aldrich/Merck). LCLs (LCL#1 and LCL#89) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in suspension at 250,000 cells per well in the presence of viral supernatant and polybrene (Sigma-Aldrich) at a final concentration of 10µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell extracts were prepared by incubating cells on ice with 3 pellet volumes of 1X lysis buffer: Cell signalling 10X whole cell lysis buffer diluted to 1X and supplemented with pepstatinA (Sigma) and PhospSTOP™ (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... and then seeded into 6-well plates (800,000 cells per well) or 12-well plates (400,000 cells per well) coated with 1% w/v gelatin (G9391, Sigma-Aldrich) in plating media overnight to allow cardiomyocyte attachment.
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bioRxiv - Cell Biology 2023Quote: ... Concentrated virus equivalent to that harvested from half a 10 cm dish of HEK293T cells was added to 2 × 105 BEL-A cells in the presence of 8 μg/mL polybrene (Sigma) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... briefly the cells were cultured and after attaining 80-90% confluency the cells were lysed in 100 µl RIPA buffer (Sigma) containing protease and phosphatase Inhibitor ...
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bioRxiv - Biophysics 2023Quote: African green monkey kidney fibroblast-like cells (COS7, Cell Lines Service GmbH, Eppelheim, #605470) were cultured in DMEM (Sigma, #D8062) containing 10% FCS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Cell counting was conducted by adding 20 µL of the cell suspension to 20 µL Trypan Blue (Sigma #T8154-100ML), and cell numbers were determined with the Countess® II cytometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... HT1080 and MRC5 (purchased from ATCC) cells and corresponding telomere elongated cells were maintained in Modified Eagle’s medium (MEM) (Sigma-Aldrich) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Cell Biology 2024Quote: ... The percent of live or dead cells was determined for cells treated with vehicle or 5 μM PF-04620110 (DGAT1, Sigma) and 5 μM PF-06424439 (DGAT2 ...
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bioRxiv - Bioengineering 2024Quote: ... the bone marrow mononuclear cell fraction and the stromal cell fraction post Ficoll-density gradient centrifugation (Histopaque 1077; Sigma-Aldrich) were quantified with an automated electrical impedance-based CASY® Cell Counter (Schaerfe System GmbH) ...
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bioRxiv - Immunology 2024Quote: Mouse cell lines: HC11 cells (CRL-3062, ATCC) were cultured in RPMI-1640 and supplemented with 10% FBS (F2442, Sigma) and 100 U/ml penicillin ...
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bioRxiv - Cancer Biology 2024Quote: ... cells in a single well were trypsinized and live cell count obtained by staining with 0.4% trypan blue (Sigma, T8154) and counted using the BioRad TC20 automated cell counter ...
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bioRxiv - Cancer Biology 2024Quote: Cells were cell-surfaced stained and fixed overnight with 1% PFA prior to permeabilisation with 0.3% Saponin (Sigma-Aldrich Ltd) for 15mins at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and lysed on ice for 30 min at a cell density of 107 cells per ml of RIPA lysis buffer (Merck Millipore), supplemented with phosphatase and complete protease inhibitors (Merck Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... 786-O, 769-P, A704, and RCC- FG2 (Cell Lines Service, CLS GmbH) cells were cultured in RPMI 1640 medium (Sigma) supplemented with 10% FBS and 1% P/S ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... and baby hamster kidney (BHK-21) cells (kind gift from R. Everett) cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, D6429) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... HRS/Gsc-GFP dual reporter cells at day 6 of the ADE differentiation were isolated by washing the cells twice with PBS (Sigma; suitable for cell culture ...
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bioRxiv - Immunology 2024Quote: ... We then stained cells immediately for flow analysis or cryopreserved cells in cryopreservation solution (CPS; 10% sterile DMSO (Sigma Aldrich), 90% FBS).
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bioRxiv - Immunology 2024Quote: ... We then stained cells immediately for flow analysis or cryopreserved cells in cryopreservation solution (CPS; 10% sterile DMSO (Sigma Aldrich), 90% FBS).
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bioRxiv - Developmental Biology 2024Quote: ... and the cell suspension was filtered and transferred to a new tube using a FlowMi Cell Strainer (Sigma-Aldrich, BAH13600040) (40μm ...
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bioRxiv - Cancer Biology 2024Quote: ... PANC1 and UM5 cells were constantly kept in cell culture medium containing 5 µg/ml and 2.5 µg/ml Puromycin (P8833, Millipore Sigma), respectively ...
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bioRxiv - Genetics 2024Quote: K562 cells were induced to erythroblastic cells by exposure to 3U/mL hEPO (SCT 78007) or 50 ìM hemin (Sigma). Megakaryocytic cells were obtained with 80 nM phorbol 12-myristate 13-acetate (Invivogene) ...
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bioRxiv - Evolutionary Biology 2024Quote: We chemically lysed cell pellets in 200 µl of freshly prepared lysis buffer containing Cell lytic Buffer-B (Sigma #B7435), 100 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: LX-2 human hepatic stellate cells [47] (Millipore) were cultured in DMEM supplemented with 10% fetal bovine serum at 37°C under 5% CO2 ...
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bioRxiv - Biophysics 2019Quote: ... PtK2 Cells were grown in DMEM (Sigma Aldrich) supplemented with 15% FBS (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pasaged using trypsin (T4049, Sigma-Aldrich) every 3-4 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were passaged using trypsin (T4049, Sigma-Aldrich) every 3-4 days.
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bioRxiv - Cancer Biology 2021Quote: ... and 30μg/ml endothelial cell growth supplement (Sigma). Experiments were performed using HUVECs between passage 3 and 5.
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bioRxiv - Cancer Biology 2021Quote: ... The cells were treated with doxycycline (Sigma, D9891) during the experiment.
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bioRxiv - Cancer Biology 2021Quote: ... A673 cells were cultured in DMEM (Sigma-Aldrich) and MHH-ES-1 cells in RPMI-1640 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Daoy cells were cultured in MEM (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 4 % paraformaldehyde (Sigma Aldrich) and blocked with goat serum (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: The cells were lysed in RIPA buffer (Sigma) containing protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2021Quote: Cell Counting Kit-8 (CCK-8; Sigma Aldrich) was used to measure cell viability according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained with Giemmsa (Sigma-Aldrich) prior to washing excess stain away with deionized water ...