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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the cells were resuspended to a concentration of 1×107 cells/ml in 200 ng/mL DAPI (Sigma-Aldrich) diluted in complete RPMI medium ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells in 96-well plates (100 µL cells well−1) were exposed to the extracts in DMSO (Sigma-Aldrich) /RPMI (0.25 ...
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bioRxiv - Cancer Biology 2020Quote: Cells were plated in 96-well plates at 25,000 cells/well and incubated either with JQ1 (Sigma-Aldrich SML1524) at the indicated concentrations or an equivalent volume of DMSO for 48 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... each cell suspension was brought to 105 cells/ml and supplemented with 15% (v/v) Optiprep (Sigma Aldrich, USA).
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bioRxiv - Systems Biology 2020Quote: ... The cells were disrupted by a mixture of cell lysis agent (CelLytic™ M; Sigma Aldrich, St. Louis, MO) and protease inhibitor cocktail (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... these cells were then plated (104 cells/chamber) on to invasion chambers (Sigma-Aldrich, 8.0μm pore size, TCP150-HiMedia) coated with 1 mg/mL matrigel (E1270-Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 µl of cell suspension was manually aspirated into the cell chamber pre-filled with mineral oil (Sigma-Aldrich). A fourth 2.5 ml glass syringe (SGE ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T cells were seeded in 10 cm dishes (2×106 cells) in media (DMEM High Glucose, 10% FBS (Sigma), 1% PenStrep ...
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells and red blood cells were excluded using 1 μg/ml 7-amino actinomycin D (7AAD; Sigma-Aldrich), Fixable Viability Dye eFluor® 780 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Whole-cell lysates were prepared from whole mouse brains using CST buffer (Cell Signal Technology) containing protease inhibitor (Sigma), and were rotated at 4°C for 30 minutes before DNA was pelleted ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once with PBS and red blood cells were lysed using RBC lysis buffer (Sigma-Aldrich, R7757). After neutralization with complete medium [RPMI-1640 medium (Corning ...
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bioRxiv - Immunology 2019Quote: ... Cells were incubated for 7 minutes on ice in Red Blood Cell Lysing Buffer Hybri-Max™ (Sigma-Aldrich) for the removal of contaminating erythrocytes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cultured as a monolayer in Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich, USA; for SKOV-3 cells) and Ham’s F-10 Nutrient Mix (F-10 ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... AAV vectors were produced by seeding HEK293T cells (4.5×106 cells per dish) on 90-150 Ø15cm tissue culture dishes (Sigma). Two days later ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293ET and HEK293T cells were seeded at 30,000 cells per ml onto Poly-L-Lysine (MW 30,000 - 70,000) (Sigma-Aldrich, 9155) coated plastic 8-well chamber slides (Ibidi IB-80826) ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria were isolated from a total of ~60 million (30×6-well) cells by manual cell homogenization in a specialised buffer (20 mM HEPES (pH 7.6) (Sigma), 220 mM D-mannitol (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293 cells were seeded at 8,000-10,000 cells/cm2 on type 1 collagen (10 μg/cm2, Sigma-Aldrich) coated 35 mm polystyrene dishes (Falcon ...
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bioRxiv - Microbiology 2020Quote: ... human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% FBS and 100 U/ml Penicillin-Streptomycin (complete DMEM) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells (a human embryonic kidney cell line; ATCC CRL-1573) were maintained in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) containing fetal calf serum and antibiotics.
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bioRxiv - Immunology 2021Quote: ... Cells were further purified with red blood cell lysis buffer (0.15 M NH4Cl, 1 mM KHCO3, both from Sigma) to eliminate red blood cells ...
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bioRxiv - Immunology 2021Quote: ... Red blood cells were lysed using Red Blood Cell Lysing Buffer Hybri-Max (Sigma-Aldrich, St. Louis, MO, USA). Human T cells were identified and analyzed for activation/differentiation status using the following antibodies ...
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bioRxiv - Immunology 2020Quote: ... spleen cells were collected and fused with SP2/O myeloma cells by using polyethylene glycol (PEG1450, P5402 Sigma-Aldrich). Hybridomas were selected in HAT medium (HAT = hypoxanthine-aminopterin-thymidine Supplement ...
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bioRxiv - Microbiology 2020Quote: Fungal cells were adjusted to 1 × 106 cells/mL in Roswell Parks Memorial Institute (RPMI) media (Sigma-Aldrich, UK) and biofilms formed for 4 or 24 hours at 37°C in flat-bottom wells of 96-well plates (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were transfected with YFP-Parkin and stable cells were generated by selection using G418 (Sigma, Cat # A1720). The stably expressing cells were further transfected with EBFP2-Mito-7 and cells expressing both the transgene were sorted using flow cytometer sorter equipped with a 375-nm laser for EBFP excitation (FACS Aria III) ...
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bioRxiv - Cell Biology 2020Quote: ... and HPAF-II.iPLK4-H2B-RFP cancer cells (2.2×104 cells/ml) were combined in a 0.24% methylcellulose solution (Sigma-Aldrich, M0512). Droplets containing 1000 cells were then plated on the underside of a 15 cm culture dish and left to form spheroids overnight at 37 °C ...
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bioRxiv - Genomics 2020Quote: About 10 million cells per cell line were grown and directly crosslinked on the plate with 1% formaldehyde (Sigma) for 10 minutes at room temperature and the reaction was stopped using 125nM Glycine for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The dispersed cells were centrifuged (300xg, 10 min) and red blood cells were lysed in lysis buffer (Sigma, UK). Cells were washed and centrifuged again by the addition of 10 ml of PBS (300xg ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended in 1 ml of Stemline® II Hematopoietic Stem Cell Expansion Medium (Sigma-Aldrich), supplemented with 0.1 μg/ml Human Recombinant SCF (STEMCELL) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were seeded at a density of 105 cells/mL and incubated with 2 mM Hydroxyurea (HU, Sigma-Aldrich) for 24h ...
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bioRxiv - Biochemistry 2022Quote: ... a total of 5–7.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (2–2.5 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and the transfected cells were harvested after 4 days ...
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bioRxiv - Cell Biology 2022Quote: ... Pelleted cells were resuspended in PBS 2% FBS and dead cells were stained with 7AAD (0.5 µg/mL) (A9400, Sigma) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: U2-OS osteosarcoma cells and MDA-MB-231 cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Cancer Biology 2022Quote: ... Both cell lines as well as ATRA-resistant NB4-R1 cells were maintained in RPMI-1640 (Sigma-Aldrich, Germany) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... was propagated in Vero cells and titers were determined by plaque assay on Vero cells using 1% methylcellulose (Sigma)(Stojdl et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Recombination of Cre-ERT2expressing cells was induced in ERT2-Cre-ERT2 expressing cells by administration of 0.5μM 4-hydroxytamoxifen (Sigma-Aldrich) for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... cell pellets from transformed bacterial were lysed with a cell disruptor in the presence of 2.0 mM PMSF (Sigma). Lysates were spun at 21,000 g for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cell were sorted in single-cell drop mode into a tube containing DMEM without phenol red (D4947, Sigma-Aldrich), supplemented with GlutaMAX (35050061 ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were plated at a density of 20,000 cells per Poly-DL-Ornithine (Sigma; catalog no. P0421-100MG) and Laminin (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 cells (for proliferation assay) or 1000 cells (for 3D uptake) per 20μl drop containing 4.8mg/ml methylcellulose (Sigma-Aldrich) and 20μg/ml soluble collagen I (BioEngineering ...
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bioRxiv - Molecular Biology 2022Quote: Cells were washed with DPBS prior to lysis on ice for 15 mins in RIPA cell lysis buffer (Sigma) supplemented with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The cells were resuspended in PBS and total cells and viability was determined using Trypan Blue solution (Sigma, T8154) and counted using the TC20 automated cell counter (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells (HCT116, human colon cancer cell line, ATCC) were transduced with lentivirus using 8 μg/ml polybrene (Sigma). 72 hours after lentivirus transduction ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining cell pellet was then resuspended 80µL/5x106 cells in “Miltenyi” buffer containing 0.5% w/v BSA (Millipore Sigma), DPBS ...
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bioRxiv - Neuroscience 2023Quote: ... followed by passing through a 70 µm Flowmi Cell Strainer and a 40 µm Flowmi Cell Strainer (Millipore Sigma). The collected flowthrough was centrifuged at 500-1000 rcf for 5 min at 4°C and pellets were resuspended in staining buffer ...
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bioRxiv - Microbiology 2023Quote: J-Lat 5A8 cells (seeded at 1×106 cells/ml) were incubated with CGS21680 (Sigma-Aldrich, Cat. #C141-5MG) or SCH-58261 (Sigma-Aldrich ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... #347583) per 106 cells was added and cells were stained with 10 µg/mL propidium iodide (PI) (Sigma, #P4170) supplemented with 100 µg/mL RNase-A (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The electroporated mixture was transferred into a 1.5 mL tube to which is added PKH26 dye (0.4 µL) (PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Sigma) mixed with 100 µL diluent C ...
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bioRxiv - Bioengineering 2023Quote: Cell viability of various materials were determined using CCK-8 assay (Sigma Cell Counting Kit-8, BCCB8166, Sigma Aldrich). SH-SY5Y cells were seeded in 96-well plates at a density of 5,000 cells per well ...