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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... followed by passing through a 70 µm Flowmi Cell Strainer and a 40 µm Flowmi Cell Strainer (Millipore Sigma). The collected flowthrough was centrifuged at 500-1000 rcf for 5 min at 4°C and pellets were resuspended in staining buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Cell Biology 2023Quote: ... For each cell line 1.5 million cells were plated onto 6 cm plates and grown in DMEM (Sigma D5796) containing 10% FBS for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... human breast cancer (MCF7) and human embryonic kidney cells (HEK293T) cells were cultured in Dulbeco’s Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells and HeLa cells were incubated in Dulbecco’s modified Eagle’s medium (DMEM; D6546, Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Purified CD4+ T cells were expanded by stimulating T cells with phorbol myristate acetate (PMA, Sigma-Aldrich, 10ng/ml) and ionomycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Muse® cell analyzer kits for measurement of caspase3/7 (MCH100108) and cell cycle (MCH100106) were from EMD Millipore. Unless otherwise noted ...
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bioRxiv - Immunology 2023Quote: ... Cells were seeded at a density of 105 cells/well and pre-stimulated with PMA (200ng/ml, Sigma Aldrich).
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Bioengineering 2023Quote: Jurkat cells were cultured in cell culture medium containing 1.7 μg/ml anti-TCR antibody (Sigma-Aldrich, 05-919) overnight followed by anti-CD69 antibody staining (Biolegend ...
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bioRxiv - Systems Biology 2023Quote: ... 5,000 receiver cells were plated with 150,000 CHO-K1 wild-type cells along with the gamma-secretase inhibitor DAPT (1uM, Sigma) for use as negative controls ...
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bioRxiv - Cancer Biology 2023Quote: ... we are able to specifically label Sec residues and block Cys residues in both cell lines and tissues42,52.Cell or tissue were lysed in MES lysis buffer pH 5.6 (50mM MES (Sigma Aldrich), 150mM NaCl ...
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bioRxiv - Genomics 2023Quote: Undifferentiated shCTRL and shCPOX-4 Cells seeded at 0.5M cells/ml and treated with 5 μl 10mM GSK343 (Sigma, catalog no ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Debris and glial cells were removed by centrifuging cells over a 2 mL 15% bovine serum albumin (BSA, Sigma) solution in culture media at 250xg for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: FL8 cells were suspended at concentration of 106 cells/100 µL in 10% w/v methacrylated gelatin (Sigma 276685) and 0.067% w/v LAP (Sigma 900889 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid-PEI solutions were added to 293-EBNA1 cells (2 x 106 cells/mL) in Expression Medium [F17 Medium supplemented with 0.1% Kolliphor P 188 (Sigma), 6 mM L-glutamine (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Biochemistry 2024Quote: ... cells were seeded at 200,000 cells per well and immediately transfected with 30 picomoles of control siRNA (Sigma SIC001), or 15 picomoles each of two previously-validated (Otomo et al. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 75 μl PKH26 dye (0.5 μM, PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling, Sigma-Aldrich, Germany) was applied at least 5 days before imaging via oropharyngeal application ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the nuclear/cytoplasmic cell fractionation Cell Lytic™ NuCLEAR™ extraction kit were from Sigma (St. Louis, MO). Sequencing grade trypsin was purchased from Promega (Madison ...
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bioRxiv - Immunology 2024Quote: ... Bone marrow cells were filtered through a 70 μm strainer and treated with Red Blood Cell Lysis Buffer (Sigma). Single-cell suspensions of bone marrow cells were cultured in RPMI-1640 medium containing 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×104 cells/well were seeded in an XFe96 cell culture plate pretreated with poly-L-lysine (Sigma-Aldrich). The next day ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were seeded on 6-well plates (∼400,000 cells/well) coated with 0.1% gelatin (Sigma-Aldrich cat# G1393), and transfected ∼24 h later using the calcium phosphate method ...
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bioRxiv - Cell Biology 2023Quote: Cell viability assay was performed using the cell proliferation reagent Cell Counting Kit-8 (CCK-8) following the manufacturer’s protocol (Sigma). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... and the pellet cells were lysed for erythrocytes using a red blood cell lysis buffer (Sigma-Aldrich, Rehovot, Israel) (2 min ...
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bioRxiv - Biophysics 2021Quote: ... Dead cells were removed six days after T-cell isolation with a density-dependent gradient centrifugation step (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
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bioRxiv - Genomics 2021Quote: ... All samples (+/-auxin at all cell cycle stages) were stained with anti-pMPM2 antibody (Millipore, 05-368, 0.2µl/10million cells) for 50min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... The virus containing pellet was resuspended in serum-free stem-cell medium and target cells were infected in the presence of 1µg/ml polybrene (Sigma Aldrich). Two days following infection ...
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bioRxiv - Cell Biology 2020Quote: ... single cell clones were detached using cell dissociation solution and re-plated in a larger fibronectin coated plate (Sigma-Aldrich) with SFM.
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bioRxiv - Cell Biology 2020Quote: ... and IIICF/c (skin fibroblast cell line from Li Fraumeni patient 28) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies or Sigma) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 (human epithelial colorectal adenocarcinoma cell line) cells were purchased from ATCC and cultured in DMEM high glucose media (Sigma). The cells were trypsinized using 0.25% trypsin-EDTA and resuspended in the fresh media ...
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bioRxiv - Cell Biology 2020Quote: Cells were seeded at a density of 1 x106 cells/mL in RPMI containing 8 μg/mL polybrene (EMD Millipore), and then transduced with lentivirus by centrifugation at 2,200 RPM for 45 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... HCC cell lines were seeded and cultured in cell culture medium containing FITC-Dextran (0.1 mg/mL - FD40S - Sigma-Aldrich) for three days ...
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bioRxiv - Molecular Biology 2020Quote: ... A total of 55 million Jurkat cells seeded at 8 million cells ml-1 was treated with 50 µM of 4-thiouridine (Sigma) for 7 hours ...
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bioRxiv - Microbiology 2020Quote: ... Infected cultures were then either trypsinized for detection of GFP-positive cells and/or dextran-positive cells by flow cytometry on a Guava EasyCyte 6/2L bench cytometer (Millipore), or fixed with 4% paraformaldehyde and analyzed by fluorescence microscopy after labeling with antibodies specific for UIS4 (Sicgen ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated at 37 °C in a 5% CO2 cell incubator with 100µM IWR-1 or vehicle control in Stemline II haematopoietic stem cell expansion medium (Sigma, S0192) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were loaded into commercially available microfluidics chambers for haploid yeast cells (Y04C-02-5PK, Millipore-Sigma, Saint-Louis, USA) for the CellASIC ONIX2 microfluidics system (Millipore-Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were loaded into commercially available microfluidics chambers for haploid yeast cells (Y04C-02-5PK, Millipore-Sigma, Saint-Louis, USA) for the CellASIC ONIX2 microfluidics system (Millipore-Sigma ...
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bioRxiv - Genomics 2020Quote: BJ cells (Fig. 3) and A549 cells (Fig. S10) were treated with 5 μg/mL of Actinomycin D (Sigma, A9415), which inhibits transcription by halting elongation of the transcribed RNA (42 ...
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bioRxiv - Genomics 2020Quote: Electroporations were performed in 2-4 biological replicates into p2L RAR-DamA126 cells by mixing the listed DNA amounts with 5-20*10^6 cells in 300 ul EmbryoMax Electroporation Buffer (Millipore) and electroporating in a 0.4-cm electroporation cuvette using a BioRad electroporator at 230 V ...
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bioRxiv - Genomics 2020Quote: Xist and Kcnq1ot1 expression were induced in their respective cell lines by treating cells with 2 μg/ml doxycycline (Sigma). Xist was induced for 24 hours prior to crosslinking and analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... were plated onto 10 cm cell culture dishes at densities of 4×106 cells and incubated with or without PR inhibitor (10 μM Lopinavir, Sigma). HIV production was activated by adding Vorinostat (10 μM ...
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bioRxiv - Physiology 2019Quote: ... Cells were grown to a density of 1 × 106 cells per ml and treated with 10 µM ouabain (Sigma-Aldrich) or with 1 µM STS (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cells were then resuspended in 500 µL of frozen competent cell solution consisting of 2.5% (v/v) glycerol (Sigma-Aldrich) and 5% (v/v ...
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bioRxiv - Bioengineering 2019Quote: ... The cell culture media (designed for NCI-H295A cells) consisted of phenol red-free DMEM/F-12 medium (Sigma–Aldrich) containing 5% (vol/vol ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell Signalling Technology), ERK (1: 1000, Cell Signalling Technology), p-ERK (1: 1000, Cell Signalling Technology) and GAPDH (1: 5000, Sigma) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... +10% Fetal Bovine Serum +1% penicillin/streptomycin was used to halt digestion and cells were isolated by filtration through a 100 um cell strainer and plated in complete medium containing DOX (Sigma) at 1 μg/mL [40].
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bioRxiv - Developmental Biology 2019Quote: ... 400 µl of cell suspension (7.5×105 cells) were mixed with 20 mg/ml fibrinogen (Sigma, St Louis, MO, USA) and 200 U/ml thrombin (Sigma ...