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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Cancer Biology 2021Quote: ... CLK2 (1:1000, sigma, HPA055366-100UL), phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50. Beta-Tubulin (1:5000, Sigma Aldrich, #T7816) and Beta-Actin (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were used to detect the following proteins: GFP (1:200, CST, cat. # 2956,), peripherin (1:1000; Novus, cat. # NBP1-05423, NF200 (1:500, Millipore, cat # MAB5266). Primary antibodies were applied and incubated at 4 °C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-HISTONE H3 (phospho S10 ...
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bioRxiv - Neuroscience 2019Quote: ... Synaptic puncta were identified by the co-localization of pre-synaptic Vesicular Acetylcholine Transporter (VAChT) protein and the post-synaptic Shank protein in MAP2 stained neurons using rb anti-VAChT (Sigma Aldrich; 1:1000), ms anti-shank (Neuromab ...
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bioRxiv - Biophysics 2020Quote: ... the cells were fixed in 3% formaldehyde solution (37 wt.% in H2O, 252549, Sigma-Aldrich, St. Louis, MO) with 0.1% glutaraldehyde solution (25% in H2O ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in WT medium (DMEM high-glucose [Sigma], buffered with HEPES [Applichem] and Sodium bicarbonate [Sigma] ...
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bioRxiv - Biochemistry 2022Quote: ... Four out of eight WT and mutant samples were treated with proteinase K from Tritirachium album (Sigma Aldrich) (limited proteolysis ...
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bioRxiv - Pathology 2020Quote: ... Protein extracts (20-30 μg) were resolved by SDS-PAGE (6.0 or 7.5 % wt/vol) and transferred electrophoretically to PVDF membranes (Millipore). After blocking with non-dry fat milk (5 % wt/vol) ...
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bioRxiv - Biophysics 2019Quote: ... Primary neurons were plated at density of 100k neurons per glass coverslips coated with 0.05 mg/ml Poly-D-Lysine (mol wt 70,000-150,000, Millipore-Sigma) and cultured in Neurobasal media supplemented with glucose ...
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bioRxiv - Bioengineering 2021Quote: ... the particles were suspended in 1x PBS containing glutaraldehyde (GA) at 2.5□wt% (Sigma-Aldrich cat. no. G6257) and kept mixing at room temperature for 3□h ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were then mixed with equal volume of 5% (wt/vol) suspension of Saccharomyces cerevisiae cells (Sigma-Aldrich) prepared in PBS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and CT26.WT cells were maintained in RPMI-1640 culture media (R8758, Sigma-Aldrich, Saint-Louis, MO, USA), whereas MDA-MB-468 ...
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bioRxiv - Cell Biology 2021Quote: WT S2 cells (1.5×106) were plated on coverslips and permeabilized with 10 μg/ml digitonin (Sigma- Aldrich) in KRB for 2h at 26°C ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in Tris-buffer (0.5 M, pH 8; TB) containing 0.025% diaminobenzidine solution (DAB wt/vol, Sigma) and 0.5% nickel ammonium sulfate (wt/vol ...
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bioRxiv - Biochemistry 2019Quote: HAP1 WT or SHMT2 KO cells were seeded in 60mm dishes and stimulated with 1mM formate (Sigma-Aldrich), 10uM A769662 (Cayman Chemicals ...
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bioRxiv - Neuroscience 2019Quote: ... UPF2fl/fl;αCaMKII:CreERT2 mice and their control littermates (UPF2wt/wt;αCaMKII:CreERT2) following induction of Cre expression by tamoxifen (Sigma) at 2-months (for LTP ...
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bioRxiv - Genomics 2021Quote: ... prior to cell attachment coverslips were treated with 0.1 % W/V poly-L-lysine (mol wt 70,000–150,000 molecular weight; Sigma-Aldrich) for 60 mins at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... These were transfected into Flp-IN 293 Trex K(AAA)21 wt and ZNF598 ko using Genejuice (Novagen). 24 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... WT and E83Q aSyn were redissolved in 20 mM aqueous ammonium acetate (Sigma-Aldrich, St. Louis, Missouri, USA) solution at pH 6.8 with a final protein concentration of 20 µM ...
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bioRxiv - Microbiology 2022Quote: ... sorbitol synchronized ring stage PfCRK4::BirA* and 3D7 WT parasites were cultured [+] or [−] of 50 uM biotin (Sigma) supplementation ...
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bioRxiv - Microbiology 2022Quote: Plasmids containing WT or mutant NS1 sequence were transfected into FreeStyle 293F cells using polyethylenimine (PEI) (40K) (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... hippocampi or cortices were extracted from E18 wild type (WT) embryos in Hanks’ balanced salt solution (Sigma Aldrich), supplemented with 10mM HEPES (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... HEK 293t cells were infected with commercially available Lentiviral particles for hTRMT2A KD and scrambled control cell line (MISSION® shRNA Lentviral Transduction Particles: scrambled control WT = SHC002V, KD1 = NM_182984.2-856s1c1, KD2 = NM_182984.2-1574s1c1; Sigma-Aldrich). Cells with stable integration of shRNA were selected as puromycin-resistant colonies ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from testis tissues (3 WT versus 3 Clpp-null)) with TRI reagent (Sigma-Aldrich), and reverse transcription was done with SuperScript IV VILO Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Neuroscience 2019Quote: ... The protein sample was incubated with taxol-stabilized microtubules (15 mg mL-1) and 1 mM AMP-PNP (Sigma) and the mixture overlaid on a glycerol cushion (BRB80 ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Biochemistry 2022Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Sigma) or 16O light (Fisher ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg mL-1 catalase from bovine liver (lyophilized powder, 2000-5000 units/mg protein, Sigma-Aldrich, Missouri, US) and 50 mM SPB (pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5□µg/ml human insulin (Sigma), 100 U/ml penicillin and 100□μg/ml streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2021Quote: ... Commercially available human serum (Sigma-Aldrich) was used as the positive control.
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
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bioRxiv - Developmental Biology 2020Quote: ... of human chorionic gonadotrophin (hCG) (Sigma) 46 hours later ...
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bioRxiv - Developmental Biology 2020Quote: ... human insulin was purchased by SIGMA and was used at a final concentration of 7ug/ml ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with human albumin (Sigma, #A0237), sodium DL-lactate (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human serum albumin (Sigma, Catalog # A9511) and human pituitary FSH (National Hormone and Pituitary Program ...
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bioRxiv - Immunology 2021Quote: ... purified human C1q (10μg/ml; Millipore) was added as a source of complement for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 0.5% human serum albumin (Sigma Aldrich). Cell number in the suspension was adjusted to achieve a λ (mean number of cells per droplet ...
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bioRxiv - Cell Biology 2020Quote: ... TANGO1 (rabbit anti–human; Sigma-Aldrich), sec16A (rabbit anti-human ...