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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: The MISSION pLKO.1-puro human TDP-43 (TRCN0000016038) and control shRNAs (Sigma Aldrich SHC007 and SHC016) were used to produce lentivirus ...
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bioRxiv - Microbiology 2021Quote: ... of human DDX21 isoform 1 (NM_004728.4) and isoform 2 (NM_001256910.1) into plasmid p3XFLAG-CMV-14 (Sigma-Aldrich), respectively ...
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bioRxiv - Immunology 2020Quote: ... Human monocytic cell line (THP-1) cells were differentiated with 100 nM phorbol myristate acetate (Sigma-Aldrich) for 3 days and then the same polarization protocol was performed ...
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bioRxiv - Immunology 2023Quote: ... anti-human IFN-γ clone 4S.B3) followed by labelling with 1:1000 diluted ExtrAvidin-Peroxidase (Sigma E2886) and developed by with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific™).
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bioRxiv - Molecular Biology 2023Quote: ... EpiLC transition was induced by plating 1×103 naive mESCs on a human plasma fibronectin (Millipore, FC010) coated plate in N2B27 medium supplemented with 20 ng/mL activin A (SCI) ...
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bioRxiv - Biophysics 2021Quote: ... Next, dissolved membrane proteins (1 nmol) and MSP (50 μL, 1 mg/mL) (MSP1E3D1, Sigma-Aldrich, No. M7074) were added to the lipid suspension and mixed for ∼1 h while rotating in the dark at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein expression was induced by 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, Cat. No. I6758) and occurred overnight at room temperature under shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins (0.5-1 mg) were precipitated using the anti-FLAG antibody (1:100, Cat. #F7425; Sigma-Aldrich), anti-HA antibody (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... All samples were then diluted with TE (1:1) and RNA and proteins were digested (RNAse, Sigma-Aldrich® ...
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bioRxiv - Genomics 2020Quote: ... 20 µl protein extracts diluted 1:1 (v/v) with ice-cold 2x Laemmli sample buffer (Sigma-Aldrich) were separated by 12% SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected with anti-γH2A (1:1000) and mouse anti-Ef1α clone CBP-KK1 (1:25,000; Millipore) primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein samples were diluted at a ratio of 1:1 in Laemmli sample buffer (Sigma) and separated on pre-cast gel (4–12% Tris–glycineBio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton 100 and 1 × protein inhibitor) and subsequently precipitated with anti-Flag M2 Magnetic beads (Sigma, M8823) or GFP-Trap (Chromotek ...
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Biochemistry 2024Quote: ... and human apo-transferrin (P02787) isolated from human plasma were purchased from Sigma Aldrich (E0518), Invitrogen (RP-43132) ...
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bioRxiv - Biophysics 2022Quote: ... gels were stained for 1 h using ReadyBlueTM Protein Gel Stain (Sigma-Aldrich) and destained with ddH2O overnight ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein levels were normalized to β-actin (1:5000 dilution, A5060, Sigma Aldrich). Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich), were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by western blot using anti-FLAG (1:1,000 dilution; Sigma), anti-hemagglutinin (HA ...
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bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were eluted with 150 μg.ml-1 3× FLAG peptide (Sigma-Aldrich, F4799) in extraction buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were prepared in RIPA buffer (containing protease (1:100; Sigma, P8340) and phosphatase inhibitors (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... while rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) was used for the evaluation of astrocytes ...
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bioRxiv - Plant Biology 2021Quote: ... and protein expression was induced by addition of doxycycline 1μg.mL-1 (PHR1145, Sigma). 6 hours after doxycycline addition ...
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bioRxiv - Cancer Biology 2021Quote: ... and loading control protein □-Tubulin (Sigma Aldrich ref: ref T9026, diluted 1/20,000). The secondary antibodies used were anti-mouse IgG ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-heat shock protein 70 (HSP70) (H5147, Sigma-Aldrich, 1:500), rabbit monoclonal anti-Alix (MCA2493 ...
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bioRxiv - Neuroscience 2022Quote: ... Misfolded proteins were detected by means of thioflavin-S (Sigma Aldrich; 1:1000) staining ...
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bioRxiv - Plant Biology 2021Quote: ... 8 μg of purified proteins were incubated with 1 mM DMAPP (Sigma-Aldrich) and 1 mM AMP or ATP for two hours at 30°C in 12.5 mM Tris–HCl (pH 7.5) ...
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bioRxiv - Developmental Biology 2021Quote: ... proteins were precipitated twice by addition of 1 mg of gamma-globulins (Sigma) and 2 ml 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were incubated with primary antibodies against Foxp4 (Millipore, #ABE74, 1:1000), Ucp1 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein was also visualized using 4-Chloro-1-naphthol solution (Sigma Aldrich) which is a substrate for HRP in a reaction that results in a colored precipitate.
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rat anti-mCherry (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-glial fibrially acidic protein (GFAP, 1:100, MA360, Millipore, MA, USA) and rabbit anti-phosphorylated TrkBS478 (1:200 R-1718-50 ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were detected with the primary antibodies against Flag (1:1000, Sigma F7425), GFP (1:1000 ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins were precipitated with 1 μg of anti-Flag antibody (F-3165, Sigma) for 2 h at 4°C followed by the addition of 15 μl protein G-Sepharose beads for 1 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was precipitated by adding 1 volume of TCA (trichloroacetic acid, Sigma Aldrich) to 4 volumes of supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-microtubule associated protein (MAP2; 1:1,000; Sigma‐Aldrich; St Louis, USA); DAPI (1:2,000 ...
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bioRxiv - Biochemistry 2020Quote: ... ATP11A protein was labeled with mouse anti-Flag antibody (1:2000, Sigma, Germany) and the endoplasmic reticulum (ER ...
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bioRxiv - Immunology 2020Quote: ... MaxiSorp plates were coated with 1 μg/mL HIV-GP120 protein (Sigma-Aldrich). Samples were diluted in an eight-point two-fold dilution series ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... antimicrotubule-associated protein 2 (MAP2) Alexa Fluor 647 (1:500, NB120-11267AF647; Millipore). Cell nuclei were stained by using Hoescht 33342 (2 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were reduced and alkylated for 1 h with 15 mM TCEP (Sigma) and 30 mM CAA (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced by the addition of tetracycline (1 μg/ml; Sigma) for desired time (determined during time course).
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were detected with anti-FLAG M2-HRP (1:3000 dilution, Sigma #A8592).
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bioRxiv - Genomics 2021Quote: ... and rabbit anti-Protein A antibodies (Sigma, Cat No. P3775, dilution 1:1000) were added ...
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bioRxiv - Cell Biology 2021Quote: ... Protein loading was controlled by Vinculin (VCL) antibody (1:5000, V3131, Sigma-Aldrich). Horseradish peroxidase conjugated goat anti-mouse IgG antibody (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein expression was induced using 200µM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma). Bacterial cells were pelleted and resuspended in 20ml Buffer A (for His-tagged ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse anti-glial fibrillary acidic protein (GFAP; 1:500, #G3893; Sigma-Aldrich). The next day sections were washed 3 times with the blocking buffer (15 min each ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of neuronal marker protein NeuN (anti-NeuN, 1:200, MAB377, Millipore) was determined to identify neuronal tdTomato expression.
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bioRxiv - Neuroscience 2022Quote: ... 10 μg protein was combined with 1 μL 10% Triton X-100 (Sigma) and brought to a volume of 33 μL in Vance buffer (225 mM D-mannitol ...