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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Human serum (Sigma-Aldrich, H4522) was thawed on ice and centrifuged at 18,000 ×g for 10 min at 4 °C to remove lipids ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for immunostaining: anti-CREST/anti-centromere antibodies (human, Antibodies Inc.; 1:100) anti-Tubulin (mouse monoclonal, Sigma; 1:5000). DNA was stained with 0.5 µg/ml DAPI (Serva ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were washed five times and incubated for 1 h with 1 µg/mL HRP-conjugated goat anti- human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with CR3022 anti-S antibody (1 ug/mL)128 overnight followed by anti-human IgG-HRP-conjugated goat secondary antibody (Sigma, 1:500) for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibody solution was prepared with a dilution of 1:100 of both rabbit anti-human anti-ZO-1 (Sigma-Aldrich, AB2272) and mouse anti-human anti-Vimentin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (EMD Millipore Corp.; Cas: 367-93-1). Cells were then incubated for 18 to 20 hours at 20°C at 200 rpm ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from wt and mutant embryos (n=30, for each genotype) using TRIzol (Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... Phage wash buffer for IPs contained 0.1% (wt/vol) IPEGAL® CA 630 (Sigma-Aldrich cat. no.I3021). Phage and antibody amounts for IPs were used as optimized by (Vogl et al. ...
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bioRxiv - Epidemiology 2019Quote: ... and WT were performed by intraperitoneal (i.p.) injection of 2 g/kg glucose ((Millipore-Sigma, MO, USA) or 0.75 unit/kg insulin (Lily ...
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bioRxiv - Epidemiology 2019Quote: ... and WT were performed by intraperitoneal (i.p.) injection of 2 g/kg glucose ((Millipore-Sigma, MO, USA) or 0.75 unit/kg insulin (Lily ...
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bioRxiv - Biophysics 2019Quote: ... Primary neurons were plated at density of 100k neurons per glass coverslips coated with 0.05 mg/ml Poly-D-Lysine (mol wt 70,000-150,000, Millipore-Sigma) and cultured in Neurobasal media supplemented with glucose ...
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bioRxiv - Molecular Biology 2019Quote: ... tert-Butyl hydroperoxide solution (Luperox), taxol, and Poly-L-Lysine (mol wt 70,000-150,000, 0.01%) were obtained from Sigma. Hoechst 33342 ...
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bioRxiv - Biochemistry 2020Quote: ... tetrabutylammonium hydroxide (TBAOH; 40 wt. % in water) and dibutylhydroxytoluene (BHT) solid were all obtained from Sigma Aldrich, Castle Hill ...
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bioRxiv - Biophysics 2020Quote: ... were immersed in 0.2 mL filtered fresh sea water solution containing 10 wt% dextran (31389, Sigma Aldrich) as a cryo-protectant ...
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bioRxiv - Immunology 2022Quote: ... This homogenate was mixed with equal volume of cold 30% (wt/vol) dextran (MW ∼60 kDa) (Sigma) in PBS and centrifuged at 10 000g for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... SVZ EGFR wt/amp cells were treated with TMZ (25 μM) and/or Azacytidine (AZA) (Sigma-Aldrich) (5 μm ...
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bioRxiv - Immunology 2020Quote: ... The isolated B cells from WT and MIM−/− mice were labeled with 1μM CFSE (21888, Sigma-Aldrich) or left unlabeled ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 600 mg/kg body weight Fluorescein isothiocyanate–dextran (average mol wt. 3,000-5,000, Sigma-Aldrich, Missouri, USA) in 0.9% saline was given to mice orally ...
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bioRxiv - Neuroscience 2021Quote: 2-dpf angpt1 KO and WT embryos were anesthetized with 0.0168% (w/v) tricaine (MS-222, Sigma) and mounted on 3% methylcellulose (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... Fixed cells were incubated with 200 μL of monomer solution (19% (wt/vol) Sodium acrylate (Sigma, 408220), 10% (wt/vol ...
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bioRxiv - Immunology 2019Quote: ... FVB and C57/Bl6J WT animals were subject to daily intraperitoneal (i.p) Sodium L-Lactate (Sigma, L7022) injections (0.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... To the partially clarified cell lysate 10% wt/vol Polyethylenimine (Product#408719, Sigma-Aldrich, St. Louis, MO), adjusted to pH 8.5 by addition of concentrated HCl was added to a final concentration 0.1% wt/vol ...
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bioRxiv - Biochemistry 2019Quote: ... 12CH2O formaldehyde was used for labeling of WT proteome and heavy 13CD2O formaldehyde (Sigma, cat. no. 596388) for the VPE0 quadruple mutant proteome ...
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bioRxiv - Genomics 2021Quote: ... coverslips were treated with 0.1 % W/V poly-L-lysine (mol wt 150,000-300,000 kDa; Sigma-Aldrich) for 60 mins at RT ...
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bioRxiv - Cell Biology 2021Quote: Mononuclear cells were isolated from the bone marrow of WT or Csf2rb−/− mice using Histopaque-1083 (Sigma) by centrifugation at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... oocysts were excysted prior to infection by incubating the oocysts with 0.75% (wt/vol) sodium taurocholate (Sigma) at 37°C for 60 min.
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bioRxiv - Immunology 2020Quote: WT and gpr35buu1892 zebrafish larvae were either left unstimulated or stimulated with 10 μM LPA (Sigma L7260) in water from 96 hpf until 120 hpf ...
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bioRxiv - Bioengineering 2022Quote: ... Lifecore) was dissolved in DI water at 2 wt% with Dowex 50W ion exchange resin (Sigma Aldrich) (5:1 resin to HA by weight ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with PBS and fixed with 4 wt.% paraformaldehyde (PFA, Sigma Aldrich cat. no. 158127) for 20 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2022Quote: ... Polymerized hydrogels were equilibrated in PBS at room temperature with and without 2.2 mM lithium phenyl-2,4,6-trimethyl-benzoylphosphinate photoinitiator (LAP; 0.06 wt%; Sigma-Aldrich) for overnight ...
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bioRxiv - Immunology 2022Quote: ... 12 µm liver sections from WT and TRIF Lps2 mice were stained with Oil Red O (Sigma) as per the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... wild type (WT) hM2 mAChR expressing FlpIn CHO cells were transfected transiently using Polyethylenimine (PEI, Sigma-Aldrich) with 10 ng per well of each the G protein TRUPATH biosensors pcDNA5/FRT/TO-Gαi1-RLuc8/pcDNA3.1-β3/pcDNA3.1-Gγ9-GFP2 giving a ratio of 1:1:1 ratio with 30 ng in total ...
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bioRxiv - Molecular Biology 2024Quote: ... H1.8-GFP was mixed with nucleosome with a 1.25 molar ratio in the presence of 0.001 % poly L-glutamic acid (wt 3,000-15,000) (Sigma-Aldrich) and incubated at 37 ºC for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... the mixture was combined with 0.5 % of (vol/vol) ammonium persulfate (Sigma Aldrich, 248614, 10 % (wt/vol) stock solution in distilled water ...
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bioRxiv - Microbiology 2024Quote: ... fixed cells were blocked and permeabilised with blocking buffer (5% wt/vol bovine serum albumin (Sigma-Aldrich) and 0.5% vol/vol Triton X-100 in PBS ...
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bioRxiv - Microbiology 2023Quote: ... we added 0.4% or 0.6% (wt/vol) mucin (type III mucin from porcine stomach; Sigma-Aldrich M1778) to BHI powder before mixing with Milli-Q water ...
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bioRxiv - Microbiology 2023Quote: ... spore is separated from cell debris by gradient centrifuge with 20% (wt/vol) HistoDenz (D2158, Sigma-Aldrich) or 50% (wt/vol ...
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bioRxiv - Neuroscience 2024Quote: ... The electrolyte solution consisted of aqueous 2.5 mmol chloroplatinic acid (H2PtCl6, 8 wt% H2O, Sigma-Aldrich, 262587). In some experiments ...
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bioRxiv - Cell Biology 2024Quote: ... WT or mutant) constructs using X-tremeGENE 9 DNA transfection reagent according to the manufacturer’s guidelines (Sigma). Transfected cells were incubated at 37°C ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 10 min at room temperature and blocked in 1% human AB serum (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Routine plaque assays were performed in duplicate in the presence of 1% pooled neutralising human serum (Sigma). Single-step infections were performed at moi 5 or 10 pfu/cell and multi-round infections at a moi of 0.005 pfu/cell ...
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bioRxiv - Cancer Biology 2021Quote: ... and then incubated overnight at 4 ℃ with primary antibodies against human Kv1.3 (1: 1000, P4497, Sigma, USA), P-p38 MAPK (1 ...
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bioRxiv - Cell Biology 2022Quote: ... They were then overlaid with 10-15 μl of 1 mg/ml Human IgG (Millipore Sigma, I4506) mixed with either 20 μg/ml Goat anti-Mouse IgG Alexa Fluor 405 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and binding detected using 1 in 25,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...