Labshake search
Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... 1 mM purified protein was mixed with 25% ammonium persulfate (APS) (Sigma-Aldrich) and 6.67 mM [Ru(II)(bpy)3]2+ (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rabbit anti-zinc transporter 3 (ZnT3 ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins of interest were detected with anti-FLAG antibody (Sigma F1805, 1:1000) and anti-PCNA antibody (Santa Cruz sc-8349 ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
-
bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
-
bioRxiv - Cell Biology 2024Quote: ... goat anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000; Sigma-Aldrich, catalogue# SAB2500462 ...
-
bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-glial-fibrillary acidic protein (GFAP) (Millipore Sigma, AB5804, 1:500) on shaker ...
-
bioRxiv - Developmental Biology 2023Quote: ... for 1 hr at 115 V before transferring to protein membrane (IPV00010, Millipore) at 50 V for 2 hr ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
-
bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
-
bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
-
bioRxiv - Neuroscience 2023Quote: ... Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID:AB_177521), Rabbit anti-Homer1 (1:1,000 ...
-
bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were detected by immunoblotting with anti-Cdh1 antibodies (1 µg/ml, Millipore) and anti-GST antibodies (0.5 µg/ml ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins in the supernatant were precipitated with 1:4 trichloroacetic acid (Sigma Aldrich). The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2024Quote: ... and the plasma membrane protein NaK1 (mouse monoclonal, Millipore, 05-369, 1:2000). Membranes were developed using the ECL Prime Western Blotting System (Amersham Protran ...
-
bioRxiv - Neuroscience 2024Quote: ... glial fibrillary acidic protein (GFAP) (1:500; Millipore Sigma; Burlington, MA; Cat#: 345860)] in blocking solution overnight at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... and mouse monoclonal anti-HCoV-OC43 nucleocapsid protein (1:1000, Millipore Sigma, MAB9012). Secondary antibodies coupled to HRP were purchased from molecular probes and Jackson Immuno-research.
-
bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
-
bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
-
bioRxiv - Biochemistry 2024Quote: ... along with packaging vectors psPAX2 (#12260) and PMD2.G (#12259) were transfected into the HEK293-FT cells using XTremeGene 9 (Sigma Aldrich, 6365779001) (A375m ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1.5 pmole protein was used for JASPer-FLAG and aromatic cage mutant and for JJ-complex and aromatic cage mutant and pre-coupled to 5 µL FLAG-M2 beads (Sigma-Aldrich) (10 µL 50% slurry ...
-
bioRxiv - Biochemistry 2019Quote: ... after which the cells were harvested by centrifugation at 5000 rpm and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
-
bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation at 5000 rpm and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
-
bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
-
bioRxiv - Neuroscience 2020Quote: ... which were then plated in black 96-well plates in the presence of 5 mM complex I substrates glutamate and malate with or without 5 µM CCCP (carbonyl cyanide m-chlorophenyl hydrazone, Sigma-Aldrich) and 1 µM TMRM (tetramethylrhodamine ...
-
bioRxiv - Microbiology 2021Quote: We resuspended wild-type and mutant yeast cells in 50 mM potassium phosphate buffer to a final concentration 5 × 107 cells/ml and supplemented the suspension with filipin (filipin complex from Streptomyces filipinensis; Sigma F9765) to a final concentration of 5 μg/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... High Five™ insect cells for the expression of PP2A A/C complex were cultured in EX-CELL 405 Serum-Free medium (Millipore-Sigma) at 27°C with 130 rpm ...
-
bioRxiv - Immunology 2020Quote: ... Agarose was pelleted and placed at rotation for five minutes with Low Salt Immune Complex Wash Buffer (Millipore, Cat #20-154), then pelleted ...
-
An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
-
bioRxiv - Molecular Biology 2020Quote: ... the fluorescent-DNA labelled antibody complex was purified using 100 kDa molecular weight cut-off Amicon ultra filtration columns (Millipore #UFC510096) and stored in PBS buffer containing 0.5M NaCl at 4°C for use in SeqStain assays.
-
bioRxiv - Biochemistry 2020Quote: ... after which the cells were harvested by centrifugation and transferred to buffered complex methanol medium supplemented with 5 μM all-trans retinal (Sigma Aldrich). Expression was induced by the addition of 0.5% methanol ...
-
bioRxiv - Developmental Biology 2020Quote: ... Four to six larval body wall tissues containing muscle-epidermis complex were homogenized in 21 μl of RIPA buffer (Sigma, #R0278) supplemented with a cocktail of protease inhibitors (Complete mini ...
-
bioRxiv - Cell Biology 2022Quote: Drugs used in this study include the Arp2/3 complex inhibitor CK-666 (48) and the formin agonist IMM-01 (49) and were purchased from Sigma-Aldrich. For drug treatments prior to immunoprecipitation experiments ...
-
bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
-
bioRxiv - Neuroscience 2024Quote: Oligomycin and antimycin A (inhibitor of mitochondrial complex V and III respectively) were purchased from Sigma-Aldrich (O4876 and A8674 respectively).
-
bioRxiv - Cell Biology 2024Quote: ... a complex of siRNA and DharmaFECT 2 was prepared in serum-free DMEM (Sigma-Aldrich, St. Louis, MO, USA, Cat# D5796) at a volume of 100 μL/well according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were lysed by dounce homogenization and complex formation was initiated with the addition of 25 mU/mL Apyrase (Sigma-Aldrich) and 10 μM ET-1/ IRL1620 (GenScript ...
-
bioRxiv - Molecular Biology 2023Quote: ... Complex containing fractions were pooled and concentrated again using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Immunology 2023Quote: ... to block the respiratory chain via complex I and 2-deoxy-D-glucose (D8375-10MG; Sigma-Aldrich, St. Louis, MO, USA) of 10mM to inhibit glycolysis.
-
bioRxiv - Cell Biology 2023Quote: ... Then 0.4 µL of the secondary-readout (S-RO) complex was diluted in 200 µL EC buffer [10% ethylene carbonate (Sigma E26258), 10% dextran sulfate (Sigma D4911) ...
-
bioRxiv - Cell Biology 2024Quote: ... such as pyruvate and malate entering the chain at complex I) was measured after the addition of 5 mM pyruvate (P2256, Sigma-Aldrich) + 2 mM malate (M0875 ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
-
bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
-
bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
-
bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
-
bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
-
bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...