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Citations for Millipore Sigma :
1751 - 1800 of 2487 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Target cells were transduced with 0.5 mL of viral supernatant in 3 mL of total medium supplemented with 8 µg/mL polybrene (Merck Sigma). 2 to 4 days post transduction ...
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bioRxiv - Microbiology 2024Quote: ... [cyano-(3-phenoxyphenyl)methyl] 3-(2,2-dichloroethenyl)-2,2- dimethylcyclopropane-1-carboxylate (i. e. cypermethrin, pesticide analytical standard, CAS 52315-07-8, supplier: Sigma-Aldrich, purity ≥ 90.0% ...
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bioRxiv - Genomics 2024Quote: ... the embryos (8-16 cell stage) were washed through M2 and then placed in drops of Tyrode’s solution (Sigma). After about 30 sec ...
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bioRxiv - Molecular Biology 2024Quote: ... Target cells were transduced on two consecutive days by supplementing the lentiviral supernatant with 8 μg/mL polybrene (Sigma). One day after the final transduction ...
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bioRxiv - Molecular Biology 2024Quote: hTERT-RPE1 p53-/- Cas9 expressing DD-HA-ER-I-PpoI were transduced with the lentiviral TKOv3 library at a low MOI (∼0.30) in the presence of 8 μg/mL polybrene (Sigma)43,44 ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were crosslinked for 8 min at room temperature in 10ml PBS supplemented with 1% formaldehyde (Sigma, F8775). Cross-linking was quenched by adding glycine to a final concentration of 0.125 mM and incubating at room temperature for 1min ...
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bioRxiv - Plant Biology 2024Quote: ... Experiments assessing apoplastic pH were carried out as previously described (Barbez et al., 2017) using 8-hydroyypyrene-1,3,6-trisulfonuc acid trisodium salt (HPTS; Sigma-Aldrich). In brief ...
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bioRxiv - Bioengineering 2024Quote: The fibrin substrate was made using a concentration of 8 mg of fibrinogen from bovine plasma (Sigma Aldrich, F8630) per mL of GM+ to 0.4 U of thrombin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and the pellet was resuspended in 2 mL of ChIP buffer (33 mM Tris-HCl pH 8, 100 mM NaCl, 5 mM EDTA, 0.33% SDS, 1.67% Triton X-100, 1X protease inhibitor cocktail [Sigma; P8340]). Chromatin was sheared to approximately 200 bp to 500 bp fragments by sonication using a Bioruptor Plus (Diagenode ...
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 1 ml lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40, protease inhibitor cocktail (Sigma)) for 10 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were collected at appropriate time points from 5-8 week old hormone-primed (5 IU each, pregnant mare serum gonadotropin (Sigma) and human chorionic gonadotropin (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... cells following subjected treatments were collected and lysed in NP40 buffer (50mM Tris-HCl pH 8, 150mM NaCl, 5mM EDTA, 0.5% NP-40, 1x EDTA-free protease inhibitor (Sigma-Aldrich), 1x phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: Cells were seeded at a density of 1 x106 cells/mL in RPMI containing 8 μg/mL polybrene (EMD Millipore), and then transduced with lentivirus by centrifugation at 2,200 RPM for 45 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were collected on ice in 0.5% NP40 lysis buffer (50 mM tris-Hcl pH=8. 100 mM NaCl, 10% glycerol, EDTA-free protease inhibitor cocktail (Sigma), 10 μM of MG-132 (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug RNA was mixed with 0.025 pmole of tRNA standards (e.coli-tyr-tRNA and s.cerevisia-phe-tRNA at ration of 1:8 (Sigma-Aldrich; XX)) ...
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bioRxiv - Molecular Biology 2020Quote: ... A total of 55 million Jurkat cells seeded at 8 million cells ml-1 was treated with 50 µM of 4-thiouridine (Sigma) for 7 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... Human fibroblasts were doubly transduced with either shRNA or CRISPR vectors in consecutive days in the presence of 8 µg/ml protamine sulfate (Sigma). Transduced cells were selected by puromycin at 1 μg/ml.
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bioRxiv - Molecular Biology 2022Quote: ... 0.5×106 cells were mixed in a well of a 12-well plate with varying concentrations of supernatant containing viral particles and 8 μg/ml of polybrene (Sigma) which was then centrifuged at 2,000 rpm for 30 minutes at 30°C.
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes were transduced with equal volumes of freshly harvested LifeAct-mCherry and SIV3 lentiviral particles in the presence of 8 μg/mL of protamine (Sigma). At 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with ice-cold PBS and then lysed with freshly prepared lysis buffer composed of 8 M urea (Sigma), 0.1 M HEPES (pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... The harvested lentiviral supernatants were filtered using 0.45 μm syringe filter (Fisher) and used to infect target A549 cells (polybrene was added at a final concentration of 8 μg/ml, Sigma). To infect A549 cells for CRISPRi ...
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bioRxiv - Cell Biology 2020Quote: ... Equal protein concentrations were resolved via 8% SDS–PAGE and transferred to Immobilon-P polyvinylidene difluoride membrane (Sigma Aldrich; IPVH00010). Antibodies used ...
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bioRxiv - Neuroscience 2021Quote: ... and plated at a density of 1,000 cells per mm2 into 8-μwell plates (BioValley) coated with 50 μg/ml poly-D-lysine (Sigma Aldrich). Cultures were kept at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells and HEK293T cells at the confluency of 70%∼90% were infected with the virus in the presence of 8 μg/ml polybrene (Sigma). Puromycin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cell Biology 2022Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10 -8 to 3· 10 -5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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bioRxiv - Biophysics 2022Quote: ... of polyethylene glycol (PEG) of a range (0.4, 2, 4, 8, 20 or 35 kDa) of molecular weights (Sigma-Aldrich). GC376 (GC376 Sodium ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... mTEC were cultured from ALI day -3 to ALI day 8 with an added gamma-secretase inhibitor (DAPT, 50 μM, Sigma) or vehicle control (DMSO ...
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bioRxiv - Immunology 2022Quote: Monocytic THP-1 cells were infected at a density of 2×105 cells/ml in 48 well plates in the presence of polybrene (8 μg/ml, Sigma). Infection levels were assessed at 48 h post-infection through enumeration of GFP positive cells by flow cytometry ...
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bioRxiv - Genomics 2020Quote: ... cells were cross-linked with 1% fresh formaldehyde for 15 minutes and treated with lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.3% IGEPAL CA-630 (Sigma-Aldrich), 1X complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4×106 ESCs were allowed to attach to gelatinised 15cm dishes for 6-8 hours and treated with 1μM all-trans retinoic acid (Sigma-Aldrich) in EC-10 medium (DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2019Quote: ... pH 8) containing 3 mg/ml lysozyme (Chicken egg white; Fluka) and 1 mg/ml lipase (from Candida cylindracea; Sigma) in an Eppendorf tube ...
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bioRxiv - Biophysics 2019Quote: ... A pre-reaction mixture was prepared in MTEN buffer (5 mM MES (Sigma Aldrich, cat# 69889, CAS: 145224-94-8), 25 mM ethanolamine (Sigma Aldrich ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Cell Biology 2019Quote: ... cells that were split a day before were infected with the viral supernatants obtained from the above transfected cells and supplemented with 8 µg of polybrene/ml (Sigma) for at least 6 hours ...
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bioRxiv - Biochemistry 2019Quote: ... The virus-containing media was passed through a 0.45 μm filter and added to U937 cells in media containing 8 μg/mL polybrene (Sigma Aldrich). The cells were spun at 1000 x g for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: Cre expression and activity was induced in 6-8 week old Krt19-CreERT and Rosa26-CreERT mice by intraperitoneal injection of tamoxifen (Sigma) dissolved in corn oil (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... Supernatant was collected at 48 and 72 hours and applied to target cells with 8 μg/mL polybrene (EMD Millipore) for transduction ...
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bioRxiv - Immunology 2019Quote: ... Infection of J774A.1 cells with lentiviruses was performed at 50% confluency in a 10 cm-dish with 8 μg polybrene (Sigma) in the absence of FBS ...
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bioRxiv - Microbiology 2019Quote: ... equal amounts of newly harvested microconidia grown on V8 agar plates for 8 days were cultivated in 0.2x potato dextrose broth (PDB) (Sigma-Aldrich) for 5.5 h and 6.5 h with gentle shaking ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by loading whole cell lysates onto an 8%-10% SDS-PAGE gel for electrophoresis and then transferred to a PVDF membrane (Immobilon-P, Millipore). Membranes were incubated with primary antibodies in TBST (TBS ...
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bioRxiv - Neuroscience 2019Quote: ... in 0.1M PBS and then developed with a 8 min incubation in DAB-Nickel solution: 10mg 3,3′-diaminobenzidine (DAB) tablet (Sigma, DF905), 0.5 ml of a 2% aqueous nickel sulfate solution ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...
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bioRxiv - Cancer Biology 2019Quote: ... the viral supernatant was collected and added to cells in six-well plates with medium containing 8 μg/ml polybrene (Sigma). After 2 days’ infection ...
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bioRxiv - Immunology 2019Quote: ... They were further diluted 1 in 8 in HBSPE running buffer containing 1% (w/v) carboxymethyl-dextran sodium salt (Sigma) and analysed in the Biacore 3000 instrument at a temperature of 25°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Supernatant containing lentiviral particles was used to infect cancer cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Immunology 2021Quote: ... the semi-dry protein pellet was dissolved in 25µL of 8 M urea in 20mM EPPS (pH 8.5) (Sigma; Cat#E9502) and was then diluted with EPPS buffer to reduce urea concentration to 4 M ...