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Citations for Millipore Sigma :
1751 - 1800 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The modern DNA of each species was used for a long-range PCR (Sigma Aldrich Expand™ Long Template PCR System). Two primer pairs were used for the human mtDNA amplification (Gunnarsdóttir et al. ...
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bioRxiv - Immunology 2023Quote: ... a targeted amplicon library was generated by PCR from the standard gene expression library cDNA using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004). 50uL PCR reactions were prepared by combining 25uL ReadyMix with 2.5 uL of a 10uM stock of each primer (10x Partial Read 1 ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 to a final concentration of 0.2nM and PCR amplified for cloning into T7 phage vector arms (Novagen/EMD Millipore Inc. T7 Select 10-3 Cloning kit). Detailed protocol can be found in dx.doi.org/10.17504/protocols.io.j8nlkkrr5l5r/v1 ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Genetics 2022Quote: Genomic regions of interest were amplified by PCR (Phusion™ High-Fidelity DNA Polymerase, Thermo Scientific or KAPA2G Robust HotStart PCR, Sigma) from genomic DNA samples ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 µl of locus specific PCR product was used for indexing PCR using KOD Hot Start DNA Polymerase (EMD-Millipore, 71086-31) and TruSeq i5 and i7 indexing primers (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich) followed by SDS-PAGE and western blot detection with anti-streptavidin HRP antibody (Molecular Probes).
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was harvested for cytokine detection using MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Millipore) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Duolink® In Situ PLA Rabbit Plus and Mouse Minus probes were used in combination with Detection Reagent Green (Sigma) following manufacture’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
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bioRxiv - Microbiology 2020Quote: ... for ELISpot detection in combination with multiscreen immunoprecipitation (IP) ELISpot polyvinylidene difluoride (PVDF) 96-well plates (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The primary antibody for the detection of macrophage marker MS4A4A was obtained from Sigma-Aldrich (HPA029323; St. Louis, MO, USA), with staining was performed according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: ... SDS-PAGE was performed as previously described.[28] Western blotting using fluorescence detection was performed using Immobilon-FL PVDF membrane (Millipore), Chameleon Duo pre-stained protein ladder ...
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bioRxiv - Microbiology 2020Quote: Secreted lactate dehydrogenase (LDH) activity was measured as a correlate of cell death in culture supernatants using Cytotoxicity Detection KitPLUS (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... HBsAg (#5124A, Tokumen, Japan) phospho-IRE1α (Novus #NB100-2323) Detection was performed using the Immobilon Western Chemiluminescent HRP Substrate (Millipore) and image capture was done using the A1680 imaging system (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000, Sigma) and anti-MAPK6 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... The immunoreactivity was detected using the avidin-biotin detection method (biotinylated donkey-anti rabbit IgG (Sigma-Aldrich, Søborg, Denmark, #SAB3700966); avidin-biotin-peroxidase complex (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... except that the primary antibody for viral detection was a monoclonal antibody against the coronavirus group antigen (Milipore Sigma #MAB9013) and the secondary was a horseradish-peroxidase-conjugated goat-anti-mouse antibody (enQuire BioReagents # Q2AB1).
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine detection cells were stimulated with 25 ng/ml PMA and 750 ng/ml ionomycin (both Sigma-Aldrich) in the presence of GolgiStop (BD Biociences) ...
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bioRxiv - Microbiology 2020Quote: ... the Duolink in situ PLA probe anti-mouse MINUS and the in situ detection reagent Green (Sigma-Aldrich, Milan, Italy) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged protein detection was performed as previously reported [71] using an Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) and bands were visualized using an ImageQuant LAS 4000 imager.
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bioRxiv - Pathology 2021Quote: ... The blotted membranes were blocked with 5% non-fat milk and then incubated with specific antibodies diluted in 5% BSA using a standard immunoblotting procedure and detection by ECL (Millipore). ImageJ was used for densitometry quantification of western blot bands.
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bioRxiv - Plant Biology 2019Quote: ... Tocopherols in the extractions were visualized using fluorescence detection excitation at 290 nm and emission at 330 nm and compared with authentic standards (Sigma) and normalized to an internal control (tocol).
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.75 × optimized transcription/detection buffer (OTDB) (10× stock solution includes 400 mM Tris base pH 7.5 adjusted with HCl (Sigma-Aldrich), 60 mM MgCl2·6H2O (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were visualized by silver staining or by Western blotting and immuno-detection with specific antibodies in combination with a suitable secondary alkaline phosphatase-conjugated antibody (Sigma). mRFP ...
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bioRxiv - Molecular Biology 2023Quote: ... compute min/max intensity from image, use anisotropy coefficient for DoG, add detections to ROI-Manager, mean background subtraction, Sigma = 1.25 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... PLA reactions were subsequently performed using Duolink PLA probes for goat-mouse and goat-rabbit and Duolink In Situ Detection Reagents Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Goethite was then washed thoroughly with MilliQ water until no nitrate was detected in the supernatant (Nitrate detection strips, Supelco, Sigma). The purity of the synthesized goethite was confirmed using FTIR spectroscopy ...
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bioRxiv - Cell Biology 2023Quote: ... The blotted membranes were blocked with 5% non-fat milk and then incubated with specific antibodies diluted in 5% BSA using a standard immunoblotting procedure and detection by ECL (Millipore). ImageJ was used for densitometry quantification of western blot bands.
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bioRxiv - Cancer Biology 2023Quote: ... PLA reactions were subsequently carried out using Duolink PLA plus and minus probes for rabbit and mouse (respectively) and Duolink In Situ Detection Reagents Orange (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to 0.2 μm nitrocellulose membranes which were blocked with 5% non-fat skim milk for 1h at ambient temperature and incubated with desired antibodies diluted in 5% BSA using a standard immunoblotting procedure and detection by ECL (Millipore). Images were quantified in ImageJ by densitometry and normalized to GAPDH or Tubulin loading controls ...
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bioRxiv - Cell Biology 2024Quote: ... PLA signals were amplified by incubating cells with DNA polymerase for 100 min at 37°C (DUOLINK in Situ detection reagent red, DUO92008, Sigma). Confocal images were acquired using a Leica laser scanning confocal microscope and analyzed via Fiji software as previously described (19).
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bioRxiv - Microbiology 2020Quote: ... The bacterial solution was then incubated at room temperature (RT) for 20 min under moderate shaking in the presence of 0.5 mg/mL lysozyme (Sigma-Aldrich) at and additional 10 min after addition of 0.1 mg/mL DNase ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS for 30 min before incubation with primary antibodies for 1 h at RT in the permeabilization buffer supplemented with 0.5% normal goat serum: TFE3 (0.6 μg/ml; Sigma-Aldrich, HPA023881), LAMP1 (1/50 conditioned medium of hybridoma cells ...
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bioRxiv - Immunology 2021Quote: ... The H1568 cells were then fixed in 4% formaldehyde solution in PBS for 30 min at RT and then permeabilized with 0.2% (v/v) Triton X-100 (Sigma-Aldrich) in PBS for 10 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally detached from the mask and incubated for 1 hour at RT with a 5 μg/mL solution of fibronectin (Sigma) in NaHCO3 100 mM ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... The brain sections were dried for 20 minutes at RT and mounted on 0.1% gelatin-coated slides with FluoroshieldTM with DAPI (Sigma-Aldrich). Sections were visualized and photographed using a Zeiss Axio Imager Z1 microscope equipped with an Axiocam MR3.0 camera and Axivision 4.9.1 software ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was split in half and incubated for 1h at RT (while rotating) with either anti-FLAG M2 affinity gel (Sigma) or anti-HA affinity matrix (mAb 3F10 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed and incubated in CCF4 for 1 h at RT in the presence of 2.5 mM probenecid (Sigma-Aldrich). Live cells (propidium-iodide negative ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
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bioRxiv - Neuroscience 2021Quote: ... 4% in PBS for 15 min at RT] and subjected to staining with filipin (40 μg/mL in PBS for 2 h at RT; from 250-fold ethanolic stock solution; F9765, Sigma). Fluorescence images of cultured cells were acquired using an inverted microscope (Axiovert 135TV ...
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bioRxiv - Immunology 2021Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of pre-reverse transcription (RT) lysis mix containing 0.1 μl of NP40 (Sigma-Aldrich), 0.5 μl of RNAse Inhibitor (Thermo Fisher) ...