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Citations for Millipore Sigma :
1801 - 1850 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 5% milk in TBS with 0.05% Tween-20 for 1 h at RT before addition of primary antibodies (SRRM4, HPA052783, Sigma-Aldrich Quimica S.A. ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed after 14 days of differentiation with 4% paraformaldehyde in PBS for 10 min at room temperature (RT) and immunofluorescence staining was performed with βIII-tubulin (1:5,000 dilution, Sigma, T8660), NF200 (1:6,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at RT and then stained with a primary antibody (1:200, Nucleolin, CST, 145745; 1:400, NPM1, Sigma, B0556 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then pre-incubated for 1 h at RT in incubation buffer: 5 % normal donkey serum (EMD Millipore, USA) and 0.2 % Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... in Tris-buffered saline (TBS) for 15 min at RT and then further treated with 0.1% Triton-X-100 (Sigma-Aldrich) and 2% goat serum in TBS overnight at 4°C for improving antibody penetration ...
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bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... PBST) for 1 h at RT followed by incubating with primary antibodies (anti-PV, 1:1000-2000, P3088, Sigma-Aldrich) in 3% NGS in PBST at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were treated with a 10 min RT incubation in 0.1% Triton-X 100 (Sigma, St. Louis, MO, Cat. # X100) to permeabilize cells ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then blocked and permeabilized for 30 min at RT in blocking buffer that contained 3% bovine serum albumin (BSA) and 0.1% Triton X-100 (Sigma Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then permeabilized with 0.1% Triton X-100 for 15 min at RT and blocked with 1% bovine serum albumin and 5% goat serum (Sigma). cells were incubated with primary antibodies Esrrb (PP-H6705-00 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were grown to near confluency on 6 cm dishes and total RNA was extracted using the RNAzol RT reagent (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Cells grown on coverslips (1.5#) were incubated for 20 minutes at room temperate (RT) in PBS with 4% paraformaldehyde (Sigma-Aldrich) and washed three time for 10 minutes with PBS ...
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bioRxiv - Genomics 2022Quote: ... the slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (Sigma–Aldrich) in antifade DABCO (Sigma–Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Germany) slides were incubated for 1h at room temperature (RT) in TBS containing 10% donkey serum (Sigma-Aldrich, Vienna, Austria), 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2020Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of a pre-reverse transcription (RT) lysis mix containing 0.1 μl NP40 (Sigma-Aldrich), 0.5 μl RNAse Inhibitor (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing sections were incubated 10 min at room temperature (RT) with 0.001 % DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, Sigma, D9542) in PBS for nuclear staining ...
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bioRxiv - Biophysics 2021Quote: ... After gelation for 2 h at RT in a humidified chamber the samples were transferred into digestion buffer (8 U/ml Proteinase K (P4850, Sigma), 50 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then washed 2 x 1 h in 100% MeOH followed by overnight incubation at RT in DiChloroMethane:MeOH (DCM, Sigma – 270997) 2:1 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then incubated with primary antibodies at RT for 2 hrs before proceeding with Duolink PLA (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Blots were blocked with 5 % milk for 1 h at RT and then incubated in the anti-FLAG M2 (Sigma) primary antibody at a dilution of 1:2500 at 4 °C overnight with agitation ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated using RNAzol RT (Astral Scientific) per manufacturer’s instructions with the use of molecular grade isopropanol and ethanol (Sigma Aldrich) for RNA precipitation ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Bioengineering 2021Quote: ... cells were fixed with 3.6% (v/v) PFA for 15 min at RT in PBS and pellet sections stained with Alcian blue (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The fixing solution was removed together with the overlay after a 24-hour incubation at RT and plaques visualized by staining the monolayer with 0.1% crystal violet (#C0775; Sigma- Aldrich) diluted in a 20% ethanol (200 proof ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissues were washed 5 times in PBT and incubated for 2h at RT with secondary antibodies and 4’,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma) (1µg/mL) ...
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bioRxiv - Immunology 2019Quote: ... 0.1% [w/v] saponin in PBS) for 25 min at RT and then stained with mouse anti-flavivirus antibody (clone D1-4G2-4-15, Millipore) diluted 1:500 in FACS wash buffer for 90 min at RT ...
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bioRxiv - Bioengineering 2019Quote: ... for 20 min at room temperature (RT) followed by 3 x 5 min washes with PBS/0,1% Triton-X 100 (Sigma-Aldrich). Plates were then incubated with the respective primary antibody in PBS/0,1% Triton-X 100/1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were permeabilized with 100% methanol at -20ºC for 3 min at RT and blocked with 2.5% normal serum from donkey (Jackson ImmunoReasearch, 017-000-121) or goat (Sigma, 19H092) in 1%BSA/PBS for 1h at RT ...
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bioRxiv - Molecular Biology 2021Quote: Cells were fixed using 4% paraformaldehyde (PFA) for 10min at RT and permeabilized with 0.1% Triton x-100 (Sigma, T8787) for 10min at RT ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... after which the cells were fixed with 10% formaldehyde for 20 minutes at RT and stained with Oil-red-O (Sigma). For chondrogenesis ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then blocked for 1 hour at RT using 5% milk in TBS with 0.025% Tween 20 (Sigma, P1379) (TBS-T ...
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bioRxiv - Molecular Biology 2020Quote: ... for 15 min at room temperature (RT) with gentle shaking followed by washing in 1x PBS 0.3% Triton (Sigma #T9284) (PBS3T ...
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bioRxiv - Neuroscience 2022Quote: Coronal brain sections were permeabilized for 30 min at RT with 0.5% TritonX-100 (#66831, Carl Roth) in PBS and blocked for 30 min at RT with 0.2% BSA (#A7030, Sigma-Aldrich) and 5% donkey serum (#017-000-121 ...
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bioRxiv - Cancer Biology 2022Quote: ... were treated for 20 min RT with Poly-L-lysine solution 0.1 % (w/v) in H2O solution (cat# P8920 from Sigma-Aldrich). After two washes with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed (3 × 5 min. in PBS at RT) before staining with DAPI (1:1000; Sigma Aldrich, D9542) and AlexaFluor-488 Phalloidin (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted to 60 ng/μl and used in RT-qPCR using SYBR Green Jump Start Taq (Sigma-Aldrich) with 200 nM Forward and Reverse primers (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... approximately 1×108 cells were harvested (1400 g, 10 min, RT) and transferred to Hank’s balanced salt solution (Sigma Aldrich). The protein concentrations of the samples were determined by the BCA assay kit (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were then washed in 1X PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Microbiology 2022Quote: ... 5 A260 units of the cleared cell lysates were incubated either for 30 min at RT either with 3 µg of micrococcal nuclease (Sigma), or 300 units of RNase T1 (Fermentas ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Microbiology 2022Quote: ... in PBS for 5 min at RT and incubated with 5% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: NKCC1-HA-Flag-mVenus membrane expression and clustering was assessed with staining performed after a short fixation at room temperature (RT) in paraformaldehyde (PFA; 4% w/v; Sigma) and sucrose (20% w/v ...
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bioRxiv - Neuroscience 2022Quote: ... and then washed with PB (3 × 5 min; RT) and finally mounted on gelatinised slides and coverslipped with Mowiol DABCO (Sigma). For brightfield microscopy ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma ...
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bioRxiv - Immunology 2022Quote: ... 10 µM sections were cut and permeabilized for 60 mins at RT with 2% Triton X in PBS (Millipore Sigma). Slides were stained at 4°C overnight with a cocktail of anti-mouse CD4 PE (RM4-5) ...
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bioRxiv - Neuroscience 2022Quote: ... then secondary antibodies were applied for one hour at RT at indicated concentrations with 5 mg/mL DAPI (Sigma, D9542) applied at 1:5000 concentration as nuclear counterstain ...