Labshake search
Citations for Millipore Sigma :
1851 - 1900 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Plates were washed three times with 0.1% PBST and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary antibody (Cat No. NA934, Merck Millipore) diluted 1:1,000 in 0.05% PBST ...
-
bioRxiv - Developmental Biology 2023Quote: ... Sections were then blocked 1 hr at the room temperature (RT) in PBS with 2% Triton-X100 and 2% normal donkey serum (Sigma), followed by an incubation overnight at 4℃ with primary antibody diluted in the blocking solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were first stained for 20 min at RT with extracellular antibodies in the staining mix containing FACS buffer (dPBS (Sigma) with 2% HI FBS (Gibco ...
-
bioRxiv - Molecular Biology 2023Quote: ... and stained with a 1:5000 dilution of DAPI in PBS for 5-10 minutes at RT (DAPI was purchased from SIGMA). Alternatively ...
-
bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissues were dehydrated with methanol/PBS and incubated O/N at RT in 66% dichloromethane (DCM; cat. #: 270947; Sigma-Aldrich) and 33% methanol ...
-
bioRxiv - Cancer Biology 2023Quote: ... We washed the slides for 5 min in 2× SSC/0.1% IGEPAL at RT followed by a 3-minutes wash at 60 °C in 0.4× SSC/0.3% IGEPAL (Sigma-Aldrich Inc.), and an additional 3-minutes wash in 2× SSC/0.1% IGEPAL at RT ...
-
bioRxiv - Immunology 2023Quote: ... IL10 and normalization control GAPDH were examined by RT-qPCR using gene specific primers (Sigma Aldrich, St. Louis, MO, USA) as previously described 11 ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 hr at room temperature (RT) and incubated with mouse monoclonal anti-aggrecan neoepitope antibody (10 µg/ml; clone AF-28, MAB19310-C, Millipore) and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in primary antibody for 12-18hrs at RT (Rat anti-Substance P, 1:400, EMD Millipore, Cat # Mab356 ...
-
bioRxiv - Cell Biology 2023Quote: ... 1:200 during 1 hour at RT or MYH11 (Prestige Antibodies power by Atlas Antibodies, Sigma Aldrich, cat. nr HPA015310) overnight at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Bound material was eluted (1h at RT, shaking at 850 rpm) from anti-FLAG M2 magnetic beads (Sigma, Cat. # M8823) with homemade 10 µg/µL Strep-6xFLAG-peptide in PBS supplemented with 0.1% glyco-diosgenin ...
-
bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2023Quote: ... the membranes underwent a 30 min-blocking at RT using 1x carbo-free blocking solution (Vector) containing 1% Tween-20 (Sigma). The membranes were then blotted with 1 μg/mL biotinylated Sambucus Nigra Lectin (SNA ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were fixed for 12 min at room temperature (RT) and quenched by replacing the fixation solution with freshly made 0.2% NaBH4 (Sigma Aldrich) in PBS for 3 min ...
-
bioRxiv - Neuroscience 2023Quote: ... for 2 h at room temperature (RT) and then blocked in 0.2% Triton X-100 and 10% ChemiBLOCKER (Millipore, #2170) for another 2 h at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were rinsed with PBS and then fixed in PBS for 20 min at room temperature (RT) with 1.6% paraformaldehyde (P6148, Sigma-Aldrich). Cells were then rinsed with PBS and then permeabilized with 0.5% Triton X-100 (T8787 ...
-
bioRxiv - Immunology 2023Quote: ... lung sections were washed twice with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 min at RT and incubated in primary antibody mouse anti-laminA (Sigma Aldrich) at 1:500 overnight at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween-20 (TBS-T) and incubated for one hour at RT with Mouse anti-FLAG (M2) antibody-HRP conjugated (Sigma) diluted 1:4000 in blocking milk ...
-
bioRxiv - Plant Biology 2024Quote: ... and subjected to immunoblot analysis with the relevant primary and secondary antibodies (anti HA rabbit polyclonal, Abcam ab20084; anti AV2 CP rabbit polyclonal, DSMZ RT-0953-0645/1; anti c-Myc mouse monoclonal, Sigma M4439 ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were washed with PBS and permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
-
bioRxiv - Molecular Biology 2024Quote: ... washed twice with 800 µL IP buffer and incubated (5 min, RT) in the absence or presence of 10U benzonase (Millipore). Coenriched ...
-
bioRxiv - Cell Biology 2019Quote: PCR products were ligated into the p3XFLAGCMV10 (Sigma-Aldrich) and pEGFP-C1 (Clontech ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR-amplified DNA fragments were inserted into pET21b (Novagen) without adding a tag using NheI and XhoI restriction sites ...
-
bioRxiv - Genomics 2019Quote: ... and 1 µl of 10× PCR Buffer (Sigma Aldrich) with 0.25 units of JumpStart Taq DNA Polymerase (Sigma Aldrich ...
-
bioRxiv - Genetics 2019Quote: ... in Multiscreen PCR cleaning plates (Millipore, Burlington, Massachusetts, USA) with vacuum application (10 psi) ...
-
bioRxiv - Genomics 2020Quote: ... PCR reactions were prepared with 1.0M Betaine (Sigma-Aldrich), and were heated to 80°C before adding Taq Polymerase (Invitrogen) ...
-
bioRxiv - Neuroscience 2021Quote: ... The PCR product was ligated into pET28a(+) plasmid (Novagen), which was linearized with Nhe I and BamH I ...
-
bioRxiv - Cancer Biology 2020Quote: ... Regular Mycoplasma testing was performed using MycoLookOut PCR (Sigma). OVSAHO ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR products were cloned in the pET28b vector (Novagen) linearized with BamHI and NotI according to the sequence and ligation-independent cloning (SLIC ...
-
bioRxiv - Microbiology 2020Quote: ... KiCqStart SYBR green primers for qRT-PCR (Sigma-Aldrich) were specific for MCM2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Oligonucleotides for PCR amplification were obtained from Sigma Aldrich. For each construct the relevant open reading frame was completely verified by Sanger sequencing (GATC/Eurofins Scientific) ...
-
bioRxiv - Microbiology 2022Quote: Expand High Fidelity PCR System (Sigma-Aldrich, catalog #: 11759078001).
-
bioRxiv - Synthetic Biology 2022Quote: ... PCR primers were synthesized by Sigma Aldrich (S2 Table), longer DNA fragments and genes were ordered from GeneScript (S3 Table) ...
-
bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
-
bioRxiv - Microbiology 2020Quote: ... PCR primers and oligos were purchased from Sigma-Aldrich. Restriction enzymes were purchased from New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... The purified PCR product and pET-28a(+) (Novagen, Germany) were digested with BamH I and Xho I (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were then cloned in the pET15b (Novagen) plasmid modified compatible with GatewayTM cloning strategy ...
-
bioRxiv - Cancer Biology 2022Quote: ... Regular mycoplasma testing was performed using MycoLookOut PCR (Sigma). When were they last tested? HGSOC lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
-
bioRxiv - Microbiology 2023Quote: ... Colony PCR was performed using REDtaq Ready Mix (Sigma) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... were amplified by PCR using KOD Xtreme polymerase (Novagen) using primers (Table S5 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The PCR products were run in 2% agarose (SIGMA) gels and visualised under UV light.
-
bioRxiv - Cell Biology 2024Quote: ... All PCR was performed using KOD polymerase (EMD Millipore). To generate pPM09 ...
-
bioRxiv - Biophysics 2020Quote: The scaffolds were rinsed with PBS three times and fixed overnight with 10% formalin (Sigma). Following formalin fixation ...
-
bioRxiv - Immunology 2021Quote: ... Plates were washed (5 times with PBS-T) and developed with either AP substrate (Sigma) and read at 405 nm (AP ...
-
bioRxiv - Genetics 2021Quote: ... Coverslips were rinsed 3 times in PBS and mounted on glass slides with Fluoromount (Sigma) with DAPI (4′,6-diamidino-2-phenylindole).