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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Dead cells were removed on day 6 after T cell isolation through density-dependent gradient centrifugation (Histopaque 1119, Sigma) and experiments were conducted between day 7 and day 9 after isolation.
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bioRxiv - Immunology 2019Quote: ... Cell numbers and live cell count were determined using a BioRad TC20 automated cell counter and 0.4% Trypan Blue (Sigma). For the omentum ex vivo culture ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once with PBS and red blood cells were lysed using RBC lysis buffer (Sigma-Aldrich, R7757). After neutralization with complete medium [RPMI-1640 medium (Corning ...
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bioRxiv - Microbiology 2020Quote: ... For obtaining macrophage monolayer THP1 cells with required cell density were treated with 20ng/ml of PMA (P8139, Sigma) and after one day PMA was removed with 2 washes of RPMI.
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bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at 1E6 cells/ml in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma) and 50IU rhIL-2 (Peprotech).
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bioRxiv - Bioengineering 2020Quote: ... THP-1 cells were differentiated into macrophage-like cells with 150 nM phorbol 12-myristate 13-acetate (PMA) (Sigma) before the experiment.
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to a density of 1 million cells/mL and transfected using 293 transfection free reagent (Millipore) mixed with equal ratios of heavy and light chain encoding plasmids with 250 μg of DNA per one liter of culture ...
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bioRxiv - Neuroscience 2020Quote: ... The dissociated cells were plated at a density of 20000-30000 cells/cm2 on poly-L-lysine (P2636, Sigma) (0.2mg/ml in borate buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed 84h after the treatment start with Resazurin cell viability assays (16 µg/ml, Sigma-Aldrich). Dose response curves were simulated by nonlinear regression models and IC50 values were calculated using PRISM 9 (GraphPad Software Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed 84h after the treatment start with Resazurin cell viability assays (16 µg/ml, Sigma-Aldrich) normalized to the respective controls (vehicle alone) ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor tissue was further dissociated to single cells or small clumps of cells using Liberase™ TH (Sigma Aldrich) in Advanced DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were infected by mixing 2.106 cells in 2ml of freshly thawed lentivirus and Polybrene (Sigma-Aldrich, Cat# 107689) at a final concentration of 8 ug/ml ...
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bioRxiv - Cancer Biology 2020Quote: Human AGCT-derived KGN cells (Riken, Tsukuba, Japan) and JGCT-derived COV434 cells (Sigma-Aldrich, St. Louis, MO, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)-Ham’s F12 (Caisson ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: Human neuroblastoma cells (SH-SY5Y) were obtained from the European Collection of Cell Cultures (ECACC, Sigma-Aldrich, Dorset, UK) and grown in a 1:1 minimal essential medium (MEM ...
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bioRxiv - Microbiology 2020Quote: ... and PT cells (a sheep kidney cell line [38]) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma Aldrich), supplemented with 5% foetal calf serum (FCS ...
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bioRxiv - Genomics 2021Quote: ... IMR-90 cells were cultured in cell media supplemented with 50 μg/mL α-Amanitin (Sigma-Aldrich, cat.no. A2263) for 8 hours before PFA fixation ...
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bioRxiv - Immunology 2021Quote: ... cell pellets from transformed bacterial were lysed with a cell disruptor in the presence of 2.0 mM PMSF (Sigma). Lysates were spun at 21,000xg for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... and naïve CD4+ T cells (6 × 104 cells/well) were plated together with 0.1 nM retinoic acid (Sigma-Aldrich) and 2 ng/ml recombinant mouse TGF-β (R&D Systems) ...
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bioRxiv - Microbiology 2020Quote: ... The epithelial cell proteins that remained bound to the fungal cells were eluted twice with 6 M urea (Sigma). The proteins were then separated on 10% SDS-PAGE and transferred to immun-Blot PVDF Membrane (BIO-RAD) ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell extracts were prepared by lysing cells with RIPA lysis buffer supplemented with a proteinase inhibitor cocktail (Sigma). The nuclear and cytoplasmic proteins of PSC were extracted according to the instructions of the nuclear-cytoplasm extraction reagents (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Glioma cells were grown as attached on cell-treated vessels coated with 10ug/mL human plasma fibronectin (Millipore, FC010) and transfected with X2 (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... transfected cells were grown and sub-cultured in cell culture medium supplemented with 400 μg mL−1 G418 (Sigma), followed by single-cell fluorescence activated cell sorting using a BD FACSAria III cell sorter (Becton Dickinson ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells) or for 1 hour with 200 µM EU (HCT116 cells) before fixation with 3.7% formaldehyde (FA, Sigma) in PBS for 15 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... Cell counting and viability were performed Muse® Count & Viability Assay Kit in a Muse Cell Analyser (Merck Millipore).
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bioRxiv - Cell Biology 2020Quote: ... Enriched samples were quantified using a Neubauer cell counting chamber and a Scepter 2.0 Handheld Automated Cell Counter (Millipore) to have 2.0×108 infected RBC per sample ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured for 14 days in AdipoDiff medium (Miltenyi) and cells were stained with Oil Red O (Sigma). For osteogenic differentiation ...
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bioRxiv - Cell Biology 2022Quote: ... 2x cell pellet volume of lysis buffer and 1x cell pellet volume of acid washed glass beads (Millipore Sigma) were added to 1.5 ml tubes ...
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bioRxiv - Physiology 2022Quote: ... the tissue was passed through a 100 μm cell strainer and incubated in red cell lysis buffer (Sigma-Aldrich) for 8 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: PC-3 cells were seeded at 1,400 cells/100 μL in 96 well plates in RPMI media (Sigma-Aldrich) with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney epithelium 293T (HEK293T) cells and Hela cells were cultured in DMEM-high glucose (Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... were initiated with 4000 sorted TSPs or CD34+-enriched hematopoietic progenitor cells from cord blood and mixed with 80,000 MS5-hDLL1 cells (Millipore). The ATOs were placed in 0.4-μm cell culture inserts (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (a mouse melanoma cell line; RCB2630) were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# D6429-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Cell Biology 2022Quote: ... For mitotic cells staining we incubated cells with 1:500 Anti-phospho-Histone H3 (Ser10) Antibody (Sigma, 06-570) for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: The viability of THP-1 cells was assessed for 24h using the Cell Proliferation Kit II (XTT) (Sigma-Aldrich) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Parental HepG2 cells (ATCC #85011430) and HepG2-derived KO cell lines were cultured in high-glucose DMEM (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... and hippocampal cells on 22 mm glass coverslips (150,000 cells/coverslip) coated with poly-L-lysine (P2636; Sigma-Aldrich) in 2 ml plating medium (Neurobasal medium (21103-049 ...
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bioRxiv - Cancer Biology 2019Quote: Human colorectal adenocarcinoma cell lines HT29 and HCT116 (both European Collection of Cell Cultures through Sigma-Aldrich, Dorset, UK) were grown in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed with PBS prior to cell lysis and immunoblotting with the anti-puromycin 12D10 antibody (Merck Millipore). For flow cytometry (flow ...
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bioRxiv - Neuroscience 2021Quote: Cultured BV-2 cells or primary microglia were incubated with Latex beads (Sigma Aldrich, L4530; ∼100 beads per cell), or 500 nM fluorescently-labelled oligomeric Aβ (o-Aβ ...
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bioRxiv - Cancer Biology 2021Quote: ... We then fixed cells using 4% formaldehyde in PBS and stained cells with 0.1% crystal violet (Sigma Aldrich, C3886) diluted in water ...
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bioRxiv - Neuroscience 2022Quote: ... and seeded at a density of 2 × 106 cells per well (6-well plates) or 0.5 × 106 cells per well (24-well plates) into PDL/Laminin (Sigma)-coated plates with neuronal medium plus 0.5 μM RA ...
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bioRxiv - Immunology 2022Quote: ... Control apoptotic cells were prepared by incubating CD4 T cells with 2 μM camptothecin (Sigma-Aldrich, St. Louis, MO) overnight at 37°C.
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bioRxiv - Cell Biology 2022Quote: ... depigmened B16 cells were seeded at a density of 100 cells/cm2 in DMEM high glucose media (Sigma; D5648) with 10% FBS (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: HEK293T shHuR/shAUF1 and shControl cells (9.6 × 105 cells) in 6-well plates were treated with 5 μg/mL actinomycin D (Sigma) and harvested in Trizol as described above at the indicated time points ...
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bioRxiv - Genomics 2019Quote: ... Then the cells were washed with phosphate-buffered saline (PBS) and nonviable cells were removed by Percoll (Sigma-Aldrich) gradient centrifugation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... six organoids for each cell line and treatment condition were dissociated to single cells by incubation in 0.18% Papain (Sigma), 0.04% EDTA (Sigma ...
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bioRxiv - Microbiology 2019Quote: Epithelial cell lysates were prepared via incubation of PBS washed cell monolayers in RIPA buffer (EMD Millipore; Burlington, Massachusetts) with the addition of 1mM PMSF and 1x Protease Inhibitor Cocktails (Sigma) ...
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bioRxiv - Biophysics 2019Quote: ... 200 μL of the cell sample was incubated in 20 μL of Cell Proliferation Reagent WST-1 (Sigma Aldrich) for 1.5 h at room temperature to lyse the cells ...
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bioRxiv - Cell Biology 2019Quote: ... S2 cells were settled for 20’ on polylysine coated cells and fixed for 7’ with 3.7% formaldehyde solution (Sigma) in PBS or in −20°C methanol ...
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bioRxiv - Bioengineering 2020Quote: ... The activated cells were added to the virus-bound plates after removing dead cells using Histopaque-1119 (Sigma-Aldrich), and centrifuged at 3200 × g for 90 min at 32°C ...