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Citations for Millipore Sigma :
1751 - 1800 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... mitoProfile total OXPHOS Human WB antibody cocktail (1:1000, ab110411), SDHA monoclonal antibody (1:10000, 459200), anti-TOMM20 (1:1000, HPA011562) and actin antibody (1:1200, Sigma A2066). Four 5 min washes with PBS-T (0.005% tween ...
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bioRxiv - Developmental Biology 2021Quote: ... The antibodies: Chicken anti-GFP antibody (ab16901, 1:500; Millipore), Mouse anti-Satb2 (ab51502 ...
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bioRxiv - Systems Biology 2020Quote: ... Antibodies used included: Rac1 antibody (Millipore, clone 238A, 1:500), anti-RhoA antibody (Santa Cruz Biotechnology 26C4 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA ...
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bioRxiv - Cell Biology 2022Quote: ... phosphatase inhibitors (1 mM sodium fluoride [201154; Sigma-Aldrich], 1 mM sodium orthovanadate [S6508; Sigma-Aldrich], 5 mM sodium pyrophosphate [21368; Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were then mixed with the indicated primary antibodies and protein A/G agarose (Sigma, St. Louis, MO, USA) or anti-FLAG M2 agarose for overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-HA or α-FLAG antibodies (Sigma-Aldrich, Cat # A8592).
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of clear lysates and debris were loaded onto the same 10% Bis-Tris gel for the comparison of total protein (Coomassie staining) and ubiquitinated protein levels (anti-ubiquitin antibody, Sigma, 05-944 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... alkaline phosphatase staining was completed within each well using a commercially available Leukocyte Alkaline Phosphatase staining kit (Sigma Aldrich 86R-1KT). Whole well images were collected through brightfield microscopy and quantification of colony forming units (CFUs ...
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bioRxiv - Immunology 2019Quote: ... Osteoclast generation was verified directly by phase contrast microscopy or after staining for Tartrate-Resistant Acid Phosphatase (TRAP) (Leukocyte Acid Phosphatase kit, Sigma-Aldrich). Human TNFα and CCL2 in M-MØ culture supernatants were measured using commercially available ELISA (BD Biosciences).
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bioRxiv - Cell Biology 2022Quote: Fresh-frozen sections of young and aged bladders were tested for acid phosphatase activity using an acid phosphatase assay kit (CS0740-1KT, Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Alkaline-phosphatase (Alk-Phos) positive cells were assessed on day 18 in culture using Leukocyte Alkaline Phosphatase Kit (Sigma; cat#86R) following manufacturer instructions.
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bioRxiv - Genetics 2023Quote: ... The osteoclasts were fixed and stained with tartrate-resistant acid phosphatase (TRAP)-positive cells based on a leukocyte acid phosphatase kit (cat no 387A; Sigma; USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The 40S- and 60S-subunit fractions were mixed and concentrated using Amicon Ultra filter units (MWCO 50 kDa, Merck Millipore). The concentrated mixture was loaded onto a 10–50% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 cells/ml were co-transfected by the linearised Defbac viral backbone and the plasmids encoding for exocyst subunits or PIP5K1C using ESCORT-IV (Sigma). After 5 days ...
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bioRxiv - Biochemistry 2021Quote: ... and the fractions containing the CCT single subunit were combined and concentrated by Amicon Ultra 100 kDa MWCO centrifugal filter (Millipore). The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: Retrograde labelling of preBötC→7n neurons was achieved by either the injection of AAVrg-CAG-GFP (1.29x1013VP/ml) or Alexa Fluor 647-conjugated cholera toxin subunit B (CTB; Sigma, C34778) into 7n ...
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bioRxiv - Neuroscience 2023Quote: ... Ablation of adult M/T cells was induced by intraperitoneal injection of the diphtheria toxin A (DT) subunit (Sigma D0564) at a concentration of 20 μg/Kg body weight into Tbx::iDTR transgenic mice (Dong et al. ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were immunoprecipitated with ACC antibody (CST) or ACC2 antibody (CST) and EZview Red Protein G Affinity Gel (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:1000) and DAPI (D9542-1, Sigma, 1:10,000) were incubated for 2hrs at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... for 1.5 h at RT and incubated overnight at 4°C with primary antibodies: anti-dystrophin antibody (NCL-Dys1, 1:40, Novocastra Laboratories) and anti-alpha actinin antibody (A7732, 1:3000, Sigma-Aldrich). Membranes were incubated with the secondary antibody sheep anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies included: PGP9.5 (1:1000; AB1761-1, Millipore), ATF3 (1:400 ...
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bioRxiv - Bioengineering 2022Quote: ... Primary lubricin antibody (1:400, 1% BSA, Millipore MABT401) was incubated for 90 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
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bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... separate sets of sections were immunohistochemically processed using anti-glial fibrillary acidic protein (GFAP) monoclonal antibody 1:500 (G3893, Sigma, Saint Louis, Missouri), anti-doublecortin (DCX ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Biochemistry 2021Quote: ... followed by washing with TBST (4x 5 min) and incubation with a goat anti-mouse alkaline phosphatase secondary antibody (Sigma-Aldrich, Steinheim, Germany) diluted 1:30 000 in TBST containing 3 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The blot was washed with TBST (4x 5 min) and incubated with a goat anti-rabbit alkaline phosphatase secondary antibody (Sigma-Aldrich, Steinheim, Germany) diluted 1:30,000 in TBST containing 3 % (w/v ...
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bioRxiv - Neuroscience 2022Quote: Dephosphorylation of blots was carried out prior to application of primary antibodies by treating with bovine intestinal mucosa alkaline phosphatase (43 – 60 μg/ml, Sigma-Aldrich, Catalog # P7640) overnight.
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bioRxiv - Neuroscience 2022Quote: Dephosphorylation of brain sections and neurons was carried out prior to application of primary antibodies by treating with bovine intestinal mucosa alkaline phosphatase (43 μg/ml, Sigma-Aldrich, Catalog # P7640) overnight at 4° C.
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bioRxiv - Cell Biology 2022Quote: ... BMDMs on the dishes were washed with 5 ml of ice-cold PBS supplemented with protease inhibitor and phosphatase inhibitor (1:10 11836170001, Sigma-Aldrich, 1:100 P5726, Sigma Aldrich – PBS/c) and scratched in 2 ml PBS/c ...
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bioRxiv - Physiology 2020Quote: ... Fat tissues were homogenized in a modified radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1% protease inhibitor mixture and 1% phosphatase inhibitor mixture (Sigma-Aldrich, St. Louis, MO). Tissue lysates were resolved by SDS-PAGE ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Tissues were homogenized in a modified radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1% protease inhibitor mixture and 1% phosphatase inhibitor mixture (Sigma-Aldrich, St. Louis, MO) and tissue lysates were resolved by SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... in 50 μL of blocking buffer were added for 1 hour followed by 50 μL ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5000 dilution) in blocking buffer for 1 hour at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 minutes and incubated with a solution of 1:1 (v/v) 2X Alkaline Phosphatase buffer (10% (v/v) Polyvinyl Alcohol (Mowiol, Sigma-Aldrich, St. Louis, MO), previously filtrated and added 6.7 mL of 75 mg/mL NBT and 5 mL de 50 mg/mL BCIP) ...
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bioRxiv - Neuroscience 2023Quote: ... 215 uL of either ice-cold 20mM Tris-HCl/2 mM MgCl2/0.5% Triton X-100 with protease inhibitor (Pierce #A32953) and phosphatase inhibitor cocktail 3 (Sigma #P0044; Buffer #1, Bf#1) or boiling 1%SDS/50mM NaF (Buffer #2 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 1X protease and 1% phosphatase inhibitor prepared following the manufacturer’s protocols (Sigma-aldrich, Cat.No. 11697498001 and P5726). Cleared lysate concentrations were obtained by a DC Protein Assay (BioRad) ...