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Citations for Millipore Sigma :
2001 - 2050 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled RNAs in the eluate were then detected by autoradiography and proteins were subjected to WB using an anti-Flag antibody (Sigma-Aldrich F3165; 1:10000).
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bioRxiv - Neuroscience 2023Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2, 1:100, Millipore; mouse anti-GRIN2BB ...
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2019Quote: The bands were visualized using an acid phosphatase-conjugated secondary antibody and nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP, Sigma-Aldrich, St. Louis, USA) substrate system ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated in primary antibody of anti-HCN4 antibody (1:200, Alomone Labs, Israel) and anti connexin43 antibody (1:500, Sigma-Aldrich CO, US) for 3 nights at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-TUJ-1 antibodies (1:200; Merck-Millipore, MAB380) for 48 h ...
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bioRxiv - Zoology 2020Quote: ... We used anti-UCP-1 antibody (Sigma, U6382; 1:1000) and HRP-conjugated anti-rabbit IgG secondary antibodies (CST ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies (1:250 Anti-Fibronectin-1, F3648, Sigma-Aldrich; 1:100 Goat Anti-Type I Collagen ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.1% SDS and 50mM Tris pH 8.0) supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail (all from Sigma-Aldrich). Protein concentration was measured using Bradford assay (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... and Ypt1-7xHis were mixed in a 1:3 molar ratio and incubated overnight at 4°C with calf intestinal alkaline phosphatase (Sigma) to hydrolyze nucleotide ...
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bioRxiv - Microbiology 2020Quote: Cell lysate was prepared using lysis buffer (50mM Tris, 150mM NaCl, 1% Triton, pH=8) supplemented with Phosphatase Inhibitor Cocktail (Sigma) at a concentration of 4×106 cells in 100 μL of buffer ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated with 50 µl of goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) in PBS for 1 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 1% protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (all from Sigma Aldrich, St. Louis, MO, USA). Samples were centrifuged at 14,000 rpm for 20 minutes at 4°C ...
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bioRxiv - Genetics 2021Quote: ... The peptide bound resin was then washed 3 times with DOC buffer (1% w/v sodium deoxycholate; 50mM Tris pH9; 1X MS-SAFE Protease and Phosphatase Inhibitor, all Sigma) and stored on ice until required ...
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bioRxiv - Neuroscience 2019Quote: ... 150 mM NaCl, 1% Triton-X100, 1X Complete protease inhibitor cocktail [Roche], 1X phosphatase inhibitor cocktails 2 and 3 [Sigma]). Protein concentration was determined using BCA assays (Pierce Biotech).
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bioRxiv - Cell Biology 2020Quote: Protein lysates were prepared using RIPA lysis buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM EDTA, 1% NP-40) containing protease and phosphatase inhibitors (Sigma Aldrich). The protein lysates were separated on 8-12% polyacrylamide gels and transferred to nitrocellulose or PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... were mixed in a 1:3 molar ratio and incubated overnight at room temperature with calf intestinal alkaline phosphatase (Sigma). After 12-18h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 17 mM sodium citrate, 5 mM EDTA, 0.5 mM DTT, and protease and phosphatase inhibitor cocktail [1:100]; Sigma-Aldrich) for 10 min ...
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bioRxiv - Immunology 2023Quote: ... Alkaline phosphatase (AP)-labeled goat anti-human IgM (μ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA), AP-labeled goat anti-human IgG (γ-chain specific ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed with RIPA buffer supplemented with 1% protease/phosphatase inhibitor cocktail (PIC, Sigma-Aldrich, St. Luis, MO, USA), 40 µg lysates were separated by SDS–polyacrylamide gel electrophoresis and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.3% NP-40) containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA), followed by clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
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bioRxiv - Cancer Biology 2023Quote: Gastrocnemius muscle samples were lysed in lysis buffer (10% N-Deoxycholate, 0.1% SDS, 1% Triton X-100, 10 mM Tris pH 8, 140 mM NaCl, phosphatases/proteases inhibitors [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2023Quote: ... or in vitro cell samples were homogenized in a sucrose lysis buffer (250mM sucrose, 50mM Tris-Cl pH7.4) with 1:100 phosphatase and protease inhibitor cocktail (Sigma-Aldrich) using Qiagen TissueLyser II ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were kept in a PBS buffer containing a 1:75 ratio of phosphatase inhibitor cocktail (Sigma, Cat#P5726) and protease inhibitor cocktail (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were: rabbit anti-Barhl1 polyclonal antibody (Atlas Antibodies, HPA004809, Sigma, 1:500), goat anti-Robo3 polyclonal antibody (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies used: Antibody anti-acetylated tubulin (clone 6-11B-1, Sigma), α-tubulin (Biorad MCA77G) ...
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bioRxiv - Molecular Biology 2020Quote: ... and probed with primary antibodies: anti-RPL26 antibody (1:1,000; Sigma R0780), anti-NSUN2 (1:1,000 – 1:5,000 ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated in antibodies against acetylated tubulin antibody (Sigma, 1:1000) and SV2 (DSBH 1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... The antibodies used were: rabbit antibody against Alix (1:500; ABC40, Millipore), mouse monoclonal against CNP (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies included: mouse anti-PCNA monoclonal antibody (1:500; Sigma Aldrich), mouse anti-glutamine synthetase (1:200 ...
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bioRxiv - Biophysics 2021Quote: ... Protein bands were investigated by using anti-αS monoclonal antibody (Syn 211, Millipore Sigma) and anti-mouse horse radish peroxidase secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma) or mouse anti-Flag (Sigma) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Immunology 2019Quote: ... mouse anti-MAP2 (microtubules associated protein 2) antibody (Cat. MAB378, Millipore, Boston, MA, USA), anti-ZIKV-E antibody (Cat ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins were incubated with 50 µL agarose conjugated with anti-Myc antibody (Sigma) for 2 h ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Physiology 2021Quote: ... using the following primary antibodies: glial fibrillary acidic protein or anti-GFAP (Millipore, MAB360). Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A. ...
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bioRxiv - Microbiology 2021Quote: ... Digested proteins were analyzed by Western blotting with anti-M2 FLAG monoclonal antibody (Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein extracts were incubated with 5 μl T7 antibody (EMD Millipore; Cat # 69522) for 4 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were immunoprecipitated overnight at 4 °C with PKM2 antibody (SAB4200105, Sigma) or normal rabbit IgG (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Ten milligrams of protein was incubated with 4 μL α-myc antibody (Sigma M4439) for 1 hour at 4°C ...