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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and 0.1% phosphatase inhibitor cocktail (P0044-5ML, Sigma Aldrich). Densitometry was performed to estimate the protein expression relative to the loading control (Actin ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.1% phosphatase inhibitor cocktail (P2850, P5726, Sigma Aldrich). Cells were lysed in ice cold radio-immunoprecipitation assay (RIPA ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (CAT# 4906837001, PhosSTOP Tablets, Sigma-Aldrich)] was added to HeLa cells and mixed at 4°C for 30 min ...
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bioRxiv - Pathology 2023Quote: ... Protease and phosphatase inhibitor cocktails were from Sigma-Aldrich. Cell culture reagents ...
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bioRxiv - Biochemistry 2023Quote: ... with freshly added 1x phosphatase inhibitor cocktail (Sigma-Aldrich) and 2ul/ml Benzonase (SIGMA) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.2% NP-40) containing phosphatase and protease inhibitors (Sigma), and lysed for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... containing Protease and Phosphatase Inhibitors (Sigma-Aldrich: 490687001 & 4693124001)) ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% (vol/vol) Phosphatase Inhibitor Cocktail II (Sigma). BS buffer consists of 50mM HEPES (pH=7.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... and phosphatase inhibitor cocktail II (Millipore Sigma, 20-188). Cells were kept chilled and scraped from the dish using a cell scraper ...
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bioRxiv - Neuroscience 2023Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich). After sonication (0.5 s pulse at 20% amplitude ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with a protease and phosphatase inhibitors cocktail (Sigma). Cell lysates were centrifuged for 15 minutes at 12,000g and the resulting supernatant combined with 1X Bolt®LDS Sample Buffer (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... and phosphatase inhibitor cocktail (P0044, Sigma, St Louis, MO). Whole cell lysates were centrifuged at 12,000 x g for 10 min at 4°C to remove nuclei and cell debris ...
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bioRxiv - Cell Biology 2023Quote: ... with protease and phosphatase inhibitor cocktails (Sigma-Aldrich, UK) for 10 mins on ice ...
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bioRxiv - Immunology 2023Quote: ... 1X phosphatase inhibitor 3 cocktail (Sigma-Aldrich, P0044-5ML) and 100 mM dithiothreitol (VWR ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with proteases and phosphatases inhibitor cockbTails (Sigma Aldrich). Lysates were then subjected to SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... and in some cases with phosphatase inhibitor cocktail (Sigma) as well ...
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bioRxiv - Neuroscience 2023Quote: ... and Phosphatase Inhibitor Cocktail Set V (Merck Millipore, 524629). The samples were pipetted 10-20 times up and down until dissolved and were incubated on ice for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and Alkaline phosphatase from bovine intestinal mucosa (Sigma, P5521) were used for sample digestion ...
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bioRxiv - Immunology 2023Quote: ... 1X phosphatase inhibitor cocktail 2 (Sigma-Aldrich, P5726-5ML), 1X phosphatase inhibitor 3 cocktail (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and 10 µL 10x phosphatase inhibitor cocktail (Sigma-Aldrich). Extracted proteins were quantified by the Qubit fluorometric method (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich). Retinas were sonicated twice for 15 s while RPE was passed through a 27 1/2 G syringe needle ...
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bioRxiv - Neuroscience 2023Quote: ... 2% (v/v) Phosphatase Inhibitor Cocktail (Millipore Sigma, 524629), and 0.5% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... phosphatase inhibitor 2 and 3 (Sigma Aldrich; P5726and P0044). Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227) ...
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bioRxiv - Immunology 2024Quote: ... western blot using ExtrAvidin® alkaline phosphatase (Sigma-Aldrich) to detect the BAP tag ...
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bioRxiv - Cell Biology 2023Quote: ... and phosphatase inhibitor cocktails (Cat#524627-1EA, EMD Millipore) followed by standard Western blot assays ...
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bioRxiv - Neuroscience 2024Quote: ... protease and phosphatase inhibitors) supplemented with 0.5% Sarkosyl (Sigma) and Benzonase Endonuclease (12mM MgCl2 with 250U/Sample Benzonase ...
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bioRxiv - Molecular Biology 2024Quote: ... Protease and phosphatase inhibitor cocktails were purchased from Sigma Chemical Co ...
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bioRxiv - Cell Biology 2024Quote: ... containing cOmplete protease inhibitor and PhosSTOP phosphatase inhibitors (Sigma), using probe sonication for 2×20 seconds at 60% amplitude ...
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bioRxiv - Immunology 2024Quote: ... All plates were developed with phosphatase substrate (Sigma-Aldrich) diluted in alkaline phosphatase staining buffer (pH 9.8) ...
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bioRxiv - Microbiology 2024Quote: ... 5mM EDTA with protease and phosphatase inhibitors (ThermoFisher, Sigma), on ice ...
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bioRxiv - Cell Biology 2024Quote: ... the lysis buffer was supplemented with phosphatase inhibitors (Sigma, Cat # Cocktail 2 - P5726 and Cocktail 3-P0044 ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for immunostaining: anti-CREST/anti-centromere antibodies (human, Antibodies Inc.; 1:100) anti-Tubulin (mouse monoclonal, Sigma; 1:5000). DNA was stained with 0.5 µg/ml DAPI (Serva ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hours and incubated overnight at 4°C with the primary antibodies (1/200 anti-dystrophin antibody Abcam15277, 1/50 anti-utrophin antibody Mancho 7 or 1/500 anti a-actinin antibody, Sigma-Aldrich A7732). After washing steps with PBS-Tween 0.1% ...
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bioRxiv - Developmental Biology 2019Quote: ... Tissues were blocked in 5% normal donkey serum before an overnight incubation with the primary antibody at 4°C (1:200 for ICSBP antibody, sc-365042, Santa Cruz; and 1:100 for MEF2C antibody, SAB4501860, Sigma-Aldrich). The slides were washed twice in PBS at room temperature and applied with secondary antibody diluted 1:200 in 1% BSA prepared in PBS (biotinylated anti-mouse IgG (H+L) ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... 100uL of Pierce Protein A/G Magnetic Beads were coupled to 10 µg of antibody or control rabbit IgG antibody (Sigma). DNA was incubated with antibody-coupled beads and after uncoupling ...
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Neuroscience 2021Quote: The left caudate nucleus of one monkey (not included in the behavioral study) was infused with the retrograde tracer cholera toxin B subunit (C9903, Sigma-Aldrich) via guide cannulae ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing all 11 subunits were again pooled and concentrated with an Amicon Ultra-15 Centrifugal Filter Unit 100 kDa cut-off (Millipore, UFC910024) and subsequently dialysed against CMG buffer/250 mM KOAc overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing all 11 subunits were pooled and concentrated with an Amicon Ultra-15 Centrifugal Filter Unit with a 100 kDa cut-off (Millipore, UFC910024) and then loaded onto a 24 ml Superose 6 column equilibrated in CMG buffer/200 mM KCl ...
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bioRxiv - Microbiology 2021Quote: ... Gamma-delta T cells (γδ T cells) were separated from heparinised peripheral blood by density-gradient centrifugation with Histopaque-1077 (Sigma, Spain). Phagocytosis assay was carried out in heparinized whole blood samples incubated with fluorescein (FITC)-labelled opsonized Escherichia coli bacteria using the Phagotest kit (BD Pharmigen ...
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bioRxiv - Neuroscience 2023Quote: ... Lipopolysaccharide (LPS serotype O55:B5, gamma-irradiated) and lipoteichoic acid (LTA; from Staphylococcus aureus) were purchased from Sigma (L6529 and L2515, respectively). Recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Bioengineering 2021Quote: ... 43] using rabbit polyclonal antibody Synapsin-1 (SYN-1, 1:200; Millipore), goat anti-guinea pig antibody VGlu2 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... or 1/200 anti-RpsF (Dundee Cell Products) antibodies and 1/6000 anti-rabbit secondary antibody (Sigma). Bound antibody was visualized using ECL chemiluminescent detection reagent (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used as primary antibodies: mouse anti-vinculin (1:400; clone hVIN-1, Sigma), rabbit anti-non-muscle myosin heavy chain II-A (NMIIA ...
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bioRxiv - Neuroscience 2024Quote: ... CC1 (rabbit polyclonal antibody, 1:500, Oasis Biofarm, OB-PRB070, mouse polyclonal antibody, 1:300, Millipore, OP80), ASPA (rat polyclonal antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Serial sections for trabecular bone were obtained from the distal femur at a thickness of 5 µm followed by tartrate-resistant acid phosphatase (TRAP) staining or alkaline phosphatase (ALP) staining using TRAP kits (Fast Red TR/Naphthol AS-MX, Sigma, St. Louis, MO) and NBT/BCIP (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% beta-mercaptoethanol and protease and phosphatase inhibitors (Roche Complete Mini protease inhibitor, Sigma-Aldrich #11836153001, and Roche Complete Ultra phosphatase inhibitor, Sigma-Aldrich #05892970001), 60 µL per larva ...
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bioRxiv - Genomics 2024Quote: ... Medium containing supplements were re-fed every 3-4 days for a total of 12 days after which the cells were evaluated for morphological changes and stained for tartrate-resistant acid phosphatase (TRAP) with a commercially available leukocyte acid phosphatase kit (SIGMA, Cat. 387-A). Three replicates of differentiated cells were processed to prepare samples for ATAC-seq at 0 ...