Labshake search
Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... supplemented with phosphatase inhibitor tablets (PhosSTOP, Sigma-Aldrich) and protease inhibitor (cOmplete ULTRA Tablets ...
-
bioRxiv - Biochemistry 2023Quote: ... phosphatase inhibitor cocktail-2 (Cat#P5726, Sigma-Aldrich) at 1% (v/v) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma). Benzonase (Millipore ...
-
bioRxiv - Biophysics 2024Quote: ... and phosphatase inhibitor cocktails (Sigma, P5726, and P0044). Cells were subsequently sonicated for 5 minutes at low intensity with a Standard Bioruptor Diagenode ...
-
bioRxiv - Neuroscience 2024Quote: ... and phosphatase inhibitor (# 4906845001 PhosStop™, Sigma-Aldrich). Lysates were centrifuged for 20 min ...
-
bioRxiv - Neuroscience 2024Quote: ... and phosphatase inhibitor cocktail 3 (Sigma-Aldrich P0044)) ...
-
bioRxiv - Neuroscience 2024Quote: ... phosphatase inhibitor cocktail 3 (Sigma-Aldrich, #P0044-5ML), and PMSF (phenylmethane sulfonyl fluoride ...
-
bioRxiv - Microbiology 2023Quote: ... and an Anti-Mouse lgG-Alkaline Phosphatase (Sigma) were used as the primary and secondary antibody ...
-
bioRxiv - Physiology 2023Quote: ... and phosphatase inhibitors (Sodium orthovanadate, Sigma-Aldrich, S6508). Proteins were heated at 100 °C for 5 min in SDS loading buffer (275 mM Tris ...
-
bioRxiv - Biophysics 2023Quote: ... we inhibited phosphatases using calyculin A (Sigma-Aldrich) at 20 nM ...
-
bioRxiv - Neuroscience 2023Quote: ... phosphatase inhibitor cocktail II and III (Sigma-Aldrich); pH 7.4] and lysed for 30 min at 4°C under light agitation ...
-
bioRxiv - Immunology 2023Quote: ... and phosphatase and protease inhibitors (Sigma Aldrich, USA) followed by a preclearance step by centrifugation at 14,000 rpm (4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with protease and phosphatase inhibitor cocktails (Sigma). Subsequently ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1x phosphatase inhibitors cocktail 3 (Sigma-Aldrich)) ...
-
bioRxiv - Cancer Biology 2024Quote: ... phosphatase inhibitors 1x (Sigma-Aldrich, P5726 and P0044), PMSF 1 mM (Thermo Fisher ...
-
bioRxiv - Neuroscience 2024Quote: ... phosphatase inhibitor cocktail 2 (Sigma-Aldrich, #P5726-5ML), phosphatase inhibitor cocktail 3 (Sigma-Aldrich ...
-
bioRxiv - Pathology 2024Quote: ... supplemented with phosphatase and protease inhibitor (539132, Millipore, 1:100 ...
-
bioRxiv - Biophysics 2024Quote: ... and phosphatase inhibitor cocktails (Sigma, P5726, and P0044). Cells were subsequently sonicated for 5 minutes at low intensity with a Standard Bioruptor Diagenode ...
-
bioRxiv - Cell Biology 2024Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma). Benzonase (Millipore ...
-
bioRxiv - Cancer Biology 2023Quote: ... (pH 8.0) supplemented with phosphatase inhibitors (PhosSTOP, Sigma) and protease inhibitors (cOmplete mini ...
-
bioRxiv - Cancer Biology 2024Quote: ... supplemented with phosphatase inhibitor cocktails 2 & 3 (Sigma). Whole cell lysates were vortexed at 2,000 RPM for 15 minutes before being sonicated with 30-1 second pulses ...
-
bioRxiv - Developmental Biology 2024Quote: ... and Phosphatase Inhibitor Cocktail Set II (Millipore #524625) for 30 minutes at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... and phosphatase inhibitors (Sigma-Aldrich, P5726 and P0044). Cell lysates were then centrifuged at 14,000 rpm for 15 min at 4 °C ...
-
bioRxiv - Bioengineering 2021Quote: ... Western blot analysis was performed using a primary antibody of rabbit anti-protein A (1:25,000 dilution) (Sigma-Aldrich, St. Louis, MO) and a secondary antibody of goat anti-rabbit IgG-HRP (1:3,000 dilution ...
-
bioRxiv - Developmental Biology 2022Quote: ... SDS-PAGE and western blot analyses were used to detect proteins and modifications using the following antibodies: anti-phospho SMAD2 (Ser465/467, Sigma, 1:500), Smad2 Polyclonal Antibody (Life Technologies 1:500 ...
-
bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
-
bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
-
bioRxiv - Microbiology 2019Quote: ... Relative TLR3 protein expression levels between WT and CRISPR modified cells were obtained by measuring TLR3’s expression against the intracellular β-Actin control protein bound to an anti-β-Actin monoclonal antibody (1:300; Sigma). Protein detection in WES was accomplished according to the manufacturer’s protocol using streptavidin-HRP based methodology (ProteinSimple) ...
-
bioRxiv - Immunology 2020Quote: ... Total protein in the cell lysate was estimated using the Lowry assay and 30 µg total protein from each cell lysate was used for immunoblotting with anti-HA antibody (Cat# H9658, Sigma, 1:10000), anti-AU1 antibody (Cat# ab3401 ...
-
bioRxiv - Neuroscience 2022Quote: ... Astrocytosis was assessed by glial fibrillary acidic protein (GFAP) staining using a Cy3-conjugated anti-GFAP antibody (1:200; C 9205, Sigma, Oakville, ON). Brain levels of Bdnf and Htt were determined by western blotting using protein lysates from either whole brain or microdissected brain sections with the following antibodies ...
-
bioRxiv - Immunology 2022Quote: ... Membranes were blocked in 5% BSA in TBST and incubated overnight with primary antibodies targeting the following proteins: FLAG (M2; Sigma; 1:1000), phosphorylated tyrosine (P-Y-1000 MultiMab ...
-
bioRxiv - Neuroscience 2022Quote: ... The slices were then incubated overnight at 4ºC with primary antibodies for myelin basic protein (1:250 Millipore Cat# MAB384-1ML, RRID:AB_240837), neurofilament 160 kD medium (1:250 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
-
bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
-
bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
-
bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... EDTA 5 mM and 1 X-Triton X-100) containing phosphatase and protease inhibitors (Cocktail II, Sigma-Aldrich) to obtain total protein homogenates ...
-
bioRxiv - Immunology 2022Quote: Frozen cell pellets were resuspended in 250-500ul lysis buffer (1% either Digitonin, 1x phosphatase inhibitor cocktail (Sigma), 1x protease inhibitor cocktail (Sigma) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 100 mM NaCl and 1 mM dithiothreitol) supplemented with a cocktail of protease and phosphatase inhibitors (Sigma-Aldrich) for 10 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... After the reaction with alkaline phosphatase-labelled goat anti-rabbit IgG (Sigma; 1:1,000 dilution in dilution buffer), immunoreactive products were obtained in a substrate solution of p-nitrophenylphosphate (SIGMA FAST™ p-nitrophenyl phosphate tablet set ...
-
bioRxiv - Microbiology 2022Quote: ... Then 1 mL of lysis buffer (8 M Urea buffer, 50 mM NH4HCO3, phosphatase inhibitors (Sigma-Aldrich, P2745), and protease inhibitors cocktail (Sigma-Aldrich ...
-
bioRxiv - Pathology 2022Quote: ... respectively an anti-rat IgG conjugated to alkaline phosphatase (1:500) (Sigma-Aldrich®, Merck KGaA, Darmstadt, Germany), for 60 minutes ...
-
FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... and 10 μL of protease inhibitor (protease and phosphatase inhibitor cocktail dissolved in 1 mL water) (Sigma Aldrich), and incubated on ice for 30 min.
-
bioRxiv - Molecular Biology 2023Quote: Metabolic quenching and nucleotide phosphate metabolite pool extraction were performed by adding 1 ml ice-cold 2:2:1 acetonitrile:methanol:water with 0.1% formic acid with 1x MS-Stop phosphatase inhibitor (Sigma-Aldrich). Samples were spiked with 10 µl 100 mM mix of adenosine-15N5-5′-triphosphate ...
-
bioRxiv - Immunology 2023Quote: ... for 1 hour and then alkaline phosphatase substrate solution containing 4-nitro-phenyl phosphate (N2765-50TAB, Sigma-Aldrich) for approximately 30min ...
-
bioRxiv - Cell Biology 2023Quote: ... 300 mM sucrose, 1.5 mM MgCl2, 5 mM EDTA, 0.5% Triton X-100, 1× protease and phosphatase inhibitor, Sigma) and incubated on ice for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA). Following clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
-
bioRxiv - Molecular Biology 2020Quote: ... Detection was performed by chemiluminescence (Vilber-Lourmat Fusion Solo S equipment) using an anti-fluorescein antibody coupled to alkaline phosphatase (Sigma-Aldrich, #11426338910) and using CDP-star (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... Wells were washed again and probed with 100 μl/well of a α-mouse alkaline phosphatase-conjugated secondary antibody (Sigma Aldrich, A4187) diluted 1:2,000 and incubated for one hour at room temperature ...