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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... containing 1x PhosSTOPTM phosphatase inhibitor (Sigma Aldrich) / cOmpleteTM protease inhibitor (including EDTA ...
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bioRxiv - Bioengineering 2020Quote: ... PCM-1 antibody (1:400, Sigma-Aldrich) and concentration matched rabbit IgG isotype control (Novus Biologicals ...
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bioRxiv - Biochemistry 2021Quote: ... diluted to 1:2000 was used for detection with Sigmafast BCIP/NBT alkaline phosphatase substrate at 1 mg/mL (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in urea buffer (50mM Tris HCl pH 7.5, 5mM EDTA, 6M Urea, 1% SDS, 1mM PMSF, 1:100 Phosphatase Inhibitor Cocktail 3 [Sigma-Aldrich P0044]) and cells were lysed by bead beating ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... proteins were extracted by 100 μL RIPA Lysis Buffer (P0013B, Beyotime) containing 1% Phenylmethanesulfonyl fluoride (PMSF, Beyotime) and 1% phosphatase inhibitors (PhosSTOP, Sigma-Aldrich). Using TGX Stain-Free FastCast Acrylamide Kit (1610183 ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 mg of snap-frozen PDAC tumour tissue was homogenised in 1 mL of cold 1x RIPA buffer containing 1% protease/phosphatase inhibitor cocktails (Sigma-Aldrich, cat ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour followed by three washing steps ((0.1 % Nonident-P40 and 1% phosphatase inhibitor cocktails I and II (Sigma-Aldrich, USA) in TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with fresh protease inhibitors (3 µg/mL Leupeptin, 1 µg/mL macroglobulin, 1 µM benzamidine, 0.5 µM PMSF) and phosphatase Inhibitors (EMD Millipore, 524625). Cell lysates were incubated 10 minutes on ice and centrifuged at 13000 rpm for 10 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... CHO cells were incubated with 6E10 and 10 μg/ml FITC-conjugated cholera toxin B subunits (Sigma, #C1655) to label lipid raft microdomains at 4°C for 1 h before fixation ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with primary antibodies to FLAG M2 (Sigma, A8592), HA 3F10 (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore, Massachussets, United States).
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Antibodies were enriched from antiserum using protein A-agarose (Sigma-Aldrich). Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma), eGFP fusion protein levels monitored using anti-GFP antibody (ab290 – abcam ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Cell Biology 2021Quote: ... and mouse GAPDH monoclonal antibody for protein loading controls (Sigma, G8795). Anti-mouse IRDye680RD and anti-rabbit IRDye800CW (Licor ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were recovered by binding to protein A/G agarose (Millipore), and the eluted DNA fragments were used directly for qPCR or subjected to high-throughput sequencing (ChIP-Seq ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against the following proteins were used: FLAG (Sigma Aldrich F1804), Streptavidin-Alexa Fluor 488 (Life S32354) ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by a monoclonal anti-FLAG antibody (Sigma, USA) or a polyclonal anti-GFP antibody (GenScript ...
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bioRxiv - Physiology 2021Quote: ... After 24 hours incubation cells were cultured in complete MD cell culture media supplemented with INF-Gamma (0.01ug/mL) and nerve growth factor (NGF, 0.1 ug/mL; N8133, Millipore Sigma) at 33°C for proliferation ...
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bioRxiv - Systems Biology 2023Quote: ... 5,000 receiver cells were plated with 150,000 CHO-K1 wild-type cells along with the gamma-secretase inhibitor DAPT (1uM, Sigma) for use as negative controls ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then incubated with anti-CDK2 monoclonal antibody (1:100) and anti-SCP1 polyclonal antibody (NB300-229, Novus, 1:100) or anti-RAD51 polyclonal antibody (pc130, Millipore, 1:100) and anti-SYCP-3 monoclonal antibody (sc-74569 ...
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bioRxiv - Molecular Biology 2023Quote: ... cell pluripotency was evaluated by assessing alkaline phosphatase (AP) activity using the leukocyte alkaline phosphatase kit (Sigma, 86R-1KT) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1% NP-40 and 2 mM EDTA) supplemented with protease and phosphatase I and II inhibitors (Sigma–Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... which in turn was detected using 1:5000 goat anti-mouse IgG alkaline phosphatase conjugate (Sigma, A3562).
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bioRxiv - Molecular Biology 2022Quote: ... In Figure 3B and Supplemental Figure 3 the extraction buffer also contained phosphatase inhibitor cocktail 1 (Sigma P2850 microcystin LR ...
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bioRxiv - Neuroscience 2024Quote: ... 50mM NaF and 1 mM phenylmethylsulfonyl fluoride (PMSF) supplemented with cocktail of protease and phosphatase inhibitors (Sigma). Primary neuronal cultures were washed in PBS 1X and then lysed in cold RIPA lysis buffer by sonication using 30% of power (relative output 0.5 ...
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bioRxiv - Cell Biology 2024Quote: ... 0.25% NaDoc and 1% NP-40) supplemented with protease inhibitor 1X (SIGMAFASTTM) and phosphatase inhibitor 1X (Sigma) and kept on ice for 30 min ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed and incubated with 100 μl/well of 1/40,000 extravidin alkaline phosphatase (Sigma) for 30 min at 20°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Fresh lysis buffer was made by adding a 1:100 phosphatase inhibitor (PHI) cocktail (524628, EMD Millipore), a protease inhibitor (PI ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...