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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with primary antibodies overnight at 4°C (TNRC6A, human index serum, 1:3000; FOXJ1, eBioscience, # 14-9965-82, 1:100; Ac-α-tub, Sigma, # T7451 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and and CD44 (SAB1405590) (mouse) at 1:400 dilution for zebrafish tissue and 1:50 dilution for human tissue (Sigma Aldrich). The following secondary antibodies were used ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary emerin (Protein-tech, cat# 10351-1-AP, 1:3,000 dilution) and γ-tubulin (Sigma, cat# T6557, 1:25,000 dilution) antibodies were incubated overnight with rocking at 4°C and secondary antibodies (goat anti-rabbit [cat# 31462 ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:350; Wako) to evaluate the inflammatory infiltrate and rabbit anti-myelin basic protein (MBP) (1:100; Millipore) to examine demyelination ...
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bioRxiv - Cell Biology 2019Quote: ... Protein eluates were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto a nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Cell Biology 2019Quote: ... The proteins of the eluate were separated by SDS PAGE and stained with Coomassie Brilliant Blue or with anti-His antibody after transfer onto nitrocellulose membrane (Sigma-Aldrich, Steinheim, Germany; dilution 1: 5000).
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with 1 ml of Stimulation Medium supplemented with 0.5 nM human insulin (Sigma) for 5 min ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... or alkaline phosphate-labeled goat-anti-human IgM (1:2000, A9794, Sigma, St. Quentin Fallavier France). Conjugate binding was quantified with SIGMAFAST p-nitrophenyl phosphate (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were washed and incubated with HRP-conjugated anti-human IgG antibody (Sigma-Aldrich; 1:10.000) followed by chemiluminescence detection.
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bioRxiv - Immunology 2020Quote: ... permeabilized RBCs were stained with anti-human spectrin antibody (1 in 50 dilution; S3396, Sigma Aldrich) concurrently with GNA lectin (8 μg/ml ...
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bioRxiv - Bioengineering 2020Quote: ... To the solution was added pre-warmed human cathepsin B (20 ng µL−1, EMD Millipore) in 30 µL MES buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... the blots were incubated with 1:20000 HRP-conjugated rabbit anti-human IgG (Sigma-Aldrich, #A8792). For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The shRNA lentiviral plasmids (pLKO.1-puro) for human/mouse/rat frataxin were purchased from Sigma. The RefSeq used was NM-008044 ...
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bioRxiv - Immunology 2020Quote: ... 30×106 PBMCs from healthy human donors were lysed in isotonic buffer containing 1% Brij58 (Sigma) on ice for 30 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... Schneider’s Insect Media (10% FBS, 1% pen/strep) supplemented with 200 mg/ml Human insulin (Sigma) was added and ovarioles were maintained at room temperature for 2-3 hours.
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bioRxiv - Biochemistry 2021Quote: ... Both samples were incubated in 1 mL of human serum from clotted whole blood (Millipore-Sigma) for 5 days at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Both samples were incubated in 1 mL of human serum from clotted whole blood (Millipore-Sigma) for 5 days at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... 1:50 polyclonal antibody against full-length human C1QBP (α-HsC1QBP) derived from mouse (Sigma-Aldrich), or both overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: IWAKI 35mm dishes (12mm glass diameter) were coated with 1:100 Human Fibronectin plasma (Sigma-Aldrich) in PBS and incubated at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged mouse and human SLFN14 was detected with anti-FLAG MAb (1/500) (M2; Sigma), non-tagged human SLFN14 was detected with antibodies anti-SLFN14 PAb (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide or conjugates were incubated with 26.6 nM human calpain-1 (Sigma-Aldrich, C6108) in 50 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES) ...
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bioRxiv - Cell Biology 2022Quote: ... per 1 ml fibrinogen at 1.5 mg/mL (plasminogen-Depleted from human plasma, Sigma-Aldrich #341578) in 1x Hanks’ buffered salt solution (HBSS ...
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bioRxiv - Cell Biology 2020Quote: ... otherwise all other protein labelling were using 1% Triton X100 (Sigma-Aldrich) in PBS permeabilization for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Guanine nucleotide-binding protein subunit alpha-11 (1:1,000; Millipore 05-855R), Guanine nucleotide-binding protein G(q ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
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bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
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bioRxiv - Molecular Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h.
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bioRxiv - Cell Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h and checked by Western blot analysis.
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bioRxiv - Genetics 2021Quote: ... clarification and benzonase treatment (250 u/1 mg protein, SIGMA E1014-5KU) extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D ...
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bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total protein was added to 7.8 nmol nitrocefin (Sigma) and absorbance at 495 nm measured kinetically for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were probed with anti-thymidylate synthase antibody (Millipore, Cat:MAB4130, 1:500), anti-CDK6 antibody (Cell Signalling ...
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bioRxiv - Cell Biology 2022Quote: ... protein expression was induced using 1 μg/mL of doxycycline (Sigma, D9891) for 16 h.
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with rabbit anti-HA (1:1,000 Sigma-Aldrich, H3663) or anti-β-actin as loading control (1:10,000 Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with mouse anti-FLAG (1:5,000 Sigma-Aldrich, F3165) or anti-β-actin (1:5,000 Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Immunohistochemistry was performed using glial fibrillary acidic protein (GFAP) (1:1000; Millipore) and ionized Ca2+-binding adapter protein (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-MBP (Myelin Basic Protein, rat polyclonal, 1:1000 Millipore MAB 386) or antimouse IgG (Biotin-SP AffiniPure ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-microtubule-associated protein 2 (MAP2) Alexa 555 (1:500, MAB3418A5; Millipore), antimicrotubule-associated protein 2 (MAP2 ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-Prosurfactant Protein C (pro-SP-C) antibody (1:4000) (Millipore, #AB3786). Antibodies were incubated in 2% powder milk in TBS pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA-protein complexes were crosslinked using 1% final concentration of formaldehyde (Sigma) for 10min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-microtubule-associated protein 2a+b (MAP2, Sigma #M1406; 1:2000), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Myelin Basic Protein antibody [12] (MBP) (Millipore; rat monoclonal, 1:500), Mouse anti-A2B5 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with anti-Flag antibody (Sigma, F1804, monoclonal, 1:1,000) or anti-Sso antibody (rabbit antiserum ...
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bioRxiv - Molecular Biology 2024Quote: ... Rabbit polyclonal antibody against protein disulfide isomerase (1:100) (PDI; P7122, Sigma). Secondary antibody Alexa Fluor 555 (A-21422 ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Glial Fibrillary Acidic Protein (1:100, Millipore, Cat# MAB360, RRID:AB_11212597), Mouse Anti-NeuN (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...