Labshake search
Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... 1% NP-40 and 2 mM EDTA) supplemented with protease and phosphatase I and II inhibitors (Sigma–Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... which in turn was detected using 1:5000 goat anti-mouse IgG alkaline phosphatase conjugate (Sigma, A3562).
-
bioRxiv - Molecular Biology 2022Quote: ... In Figure 3B and Supplemental Figure 3 the extraction buffer also contained phosphatase inhibitor cocktail 1 (Sigma P2850 microcystin LR ...
-
bioRxiv - Immunology 2023Quote: ... The wells were washed and incubated with 100 μl/well of 1/40,000 extravidin alkaline phosphatase (Sigma) for 30 min at 20°C ...
-
bioRxiv - Neuroscience 2024Quote: ... 50mM NaF and 1 mM phenylmethylsulfonyl fluoride (PMSF) supplemented with cocktail of protease and phosphatase inhibitors (Sigma). Primary neuronal cultures were washed in PBS 1X and then lysed in cold RIPA lysis buffer by sonication using 30% of power (relative output 0.5 ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.25% NaDoc and 1% NP-40) supplemented with protease inhibitor 1X (SIGMAFASTTM) and phosphatase inhibitor 1X (Sigma) and kept on ice for 30 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... cell pluripotency was evaluated by assessing alkaline phosphatase (AP) activity using the leukocyte alkaline phosphatase kit (Sigma, 86R-1KT) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
-
bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
-
bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
-
The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
-
bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
-
bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
-
bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
-
bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
-
bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
-
bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
-
bioRxiv - Genomics 2023Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
-
bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
-
bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
-
bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... the membrane was washed with TBS-T and incubated for 90 min with anti-rabbit IgG antibody conjugated to alkaline phosphatase (Sigma Alrich), diluted 1:5.000 in TBS-T solution containing 0.75 % BSA ...
-
bioRxiv - Microbiology 2020Quote: ... One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG, both from Sigma-Aldrich) diluted 1:2,000 in 1% nonfat milk-PBS were then added to each well and the plates incubated for additional 3 h at room temperature before repeating the washing step ...
-
bioRxiv - Microbiology 2021Quote: ... Wells were washed again and probed with 100 μl/well of a α-goat alkaline phosphatase conjugated antibody (Sigma Aldrich, A4187) diluted 1:2,000 and incubated for one hour at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
-
bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
-
bioRxiv - Microbiology 2020Quote: ... The secondary solution was prepared with 1% non-fat milk in PBS-T using anti-mouse IgG (whole molecule)–alkaline phosphatase (AP) antibody produced in goat (Sigma-Aldrich) at a dilution of 1:3,000 ...
-
bioRxiv - Biochemistry 2021Quote: ... the plates were then washed with PBST and incubated with the detecting antibody: alkaline phosphatase (AP)-conjugated goat anti rabbit for rabbit serum (Sigma-A8025), horseradish peroxidase (HRP)-conjugated anti-M13 antibody (GE healthcare ...
-
bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
-
bioRxiv - Bioengineering 2022Quote: ... anti-Fatty acid binding protein 1 (FABP1) (1:50, Sigma-Aldrich) and anti-cytokeratin 20 (CK20 ...
-
bioRxiv - Biochemistry 2022Quote: The proteins were visualized by incubating membranes for 30 minutes in BCIP®/NBT Alkaline Phosphatase Substrate (B5655, SIGMAFAST™ BCIP®/NBT, Sigma-Aldrich). The intensities of the detected bands were analyzed with a FluorChem 8800 Imager (Alpha Innotech ...
-
bioRxiv - Cell Biology 2023Quote: ... and protein was extracted in a modified RIPA buffer containing protease and phosphatase inhibitors (P8340 and 524624, respectively; Sigma-Aldrich, St. Louis, MO, USA). Proteins were detected by immunoblotting using standard techniques ...
-
bioRxiv - Cell Biology 2020Quote: ... All lysis buffers were supplemented with inhibitors of proteases (1/100 (v/v)) and phosphatases (1/50 (v/v)) (P8340 and P5726, Merck/Sigma-Aldrich, UK). Protein concentration was quantified using a BCA protein assay (23252 ...
-
bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
-
bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (PSD95 1:500, Antibodies Inc., Davis CA and MAP2 1:1000; Millipore) were diluted in 1x PBS with 1% BSA and 0.2% Tween ...
-
bioRxiv - Physiology 2019Quote: ... Pax7 primary antibody (1:100, DSHB) and laminin primary antibody (1:100, Sigma-Aldrich) were diluted in 1% TSA blocking buffer and applied overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... CPF-containing fractions with stoichiometric subunit amounts were pooled and concentrated in a 50 kDa Amicon centrifugal filter (Sigma) at 4000 rpm in a table top centrifuge ...