Labshake search
Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: The viabilities of the PBMCs or U-937 macrophages were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
-
bioRxiv - Biochemistry 2020Quote: ... DNA fragment spanning mccP1,P2,P3Hmi genes was amplified from genomic DNA then digested with EcoRI and KpnI and inserted into pACYCDuet-1 vector (Novagen-Millipore, USA) linearized with the same restriction endonucleases ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... the rats in SCI group were received the process of SCI and administrated with normal saline and the rats in SCI+Ex-4 group were administrated intraperitoneally (i.p.) with Ex-4 (10 µg/rat) (Sigma-Aldrich, St. Louis, MO, USA) in normal saline after SCI and dosing interval was 24h for three days ...
-
bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
-
Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Total protein staining of membrane was done using an R-PROB staining kit from Sigma. Primary antibodies were incubated overnight at 4°C and secondaries for 1 hr at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were purified using Protein G Immunoprecipitation Kit (Cat. No. IP50, Sigma-Aldrich Inc.) and were separated on SDS-PAGE for western blotting using monoclonal antibodies against T18 as described above ...
-
bioRxiv - Microbiology 2020Quote: The DRM and DSM fractions119 were separated using the CelLytic MEM Protein Extraction Kit (Sigma). 600 μl of lysis and separation buffer were added to 50 μl of resuspended membrane and incubated at 4°C overnight ...
-
bioRxiv - Immunology 2022Quote: ... The protein bands on the PVDF membranes were observed using an enhanced chemiluminescence kit (Millipore) and quantified using Image J software (NIH ...
-
bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Tissue proteins were extracted according to kit instructions (Sigma Aldrich, R0278, St. Louis, Missouri, USA) and protein concentrations were routinely quantified by using a BCA kit (Beyotime ...
-
bioRxiv - Cancer Biology 2020Quote: ... Firefly luciferase activity was normalized to protein content using Bicinchoninic Acid Kit from Sigma-Aldrich® and measured with using a luminometer (Centro XS3 LB960 ...
-
bioRxiv - Cell Biology 2021Quote: RIP assays were performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) with the mouse anti-Ago2 antibody (Millipore ...
-
bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
-
bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
-
bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentration in the extracts was measured using the QuantiPro BSA Assay Kit (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2023Quote: RIP experiment was optimized using the Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, USA) in light of the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The total protein (μg/mL/OD600) was determined using a Lowry kit (Sigma-Aldrich, TP0300), and bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2024Quote: ... and RIP assays were carried out with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore) and anti-Flag antibody (Abcam ...
-
bioRxiv - Molecular Biology 2020Quote: ... and GST-10xCTD repeats were expressed in BL21 Star (DE3) cells grown in Overnight Express Instant TB Medium (EMD Millipore) at 37°C and induced at an OD600 of 1.0 by decreasing the temperature to 18°C and growing for an additional 16 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... then scFv was expressed by changing the media to SG-Trp a synthetic galactose (SG) based media [2% galactose (Sigma), 0.2% glucose ...
-
bioRxiv - Molecular Biology 2020Quote: ... double mutant KlGal80-K5A/R6Ap (NHKlGal80-K5A/R6Ap or IHGal80-K5A/R6Ap) and KlGal1p (NHGal1p) were expressed in Escherichia coli strain Rosetta (DE3)-pLys from pET-derived (Novagen) expression vectors (pETNHG80 ...
-
bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
-
bioRxiv - Biochemistry 2019Quote: ... C-ERMAD (516-560 and 516-586) and RSK1 (696-735 and 689-735) were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with TEV-cleavable N-terminal GST-tag ...
-
bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
-
bioRxiv - Biochemistry 2020Quote: ... Neurons cultured for the indicated periods (expressed as days in vitro, DIV) were treated with LA (Cat#L1376, Sigma-Aldrich), c9,t11 CLA (Cat# 16413 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... brucei ALPH1 was expressed as an N-terminal truncation with a C-terminal 6xHis-Tag in RosettaBlue competent bacteria cells (Novagen) as previously described [10].
-
bioRxiv - Cell Biology 2022Quote: ... Adult mice were engineered with floxed alleles of Atg7 and a transgene expressing the TAM-regulated Cre recombinase fusion protein under the control of the ubiquitously expressed ubiquitin C (UBC) promoter.15 Atg7 deficiency was created by providing tamoxifen (Sigma Cat No ...
-
Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... To monitor GP63 cleavage of azocasein cleavage 50 μl of normalized live parasites (5×107) or heterologously expressed GP63 was incubated with 200μl of 50mg/ml of azocasein (Sigma, A2765) for the indicated time at 37°C ...
-
bioRxiv - Immunology 2019Quote: ... or Arf1 (using the basic CC).(38) The MBP-CC-GTPases were expressed and purified individually from BL21(DE3)T1R cells (Sigma), combined in ∼1:1 molar ratio to form a heterodimer ...
-
bioRxiv - Molecular Biology 2019Quote: ... while LMNB1-CT was expressed in Escherichia coli B834 (DE3) cultured in LB medium with 50 μg/mL of kanamycin (Sigma). EHMT1 (2IGQ ...
-
bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... and FLAG::CG33172 (pET28a-FLAG-CG33172) were co-expressed in C41 (86) bacteria and purified over Flag-coupled resin (Sigma). Bound proteins were washed 3 times in 500 mM KCl and eluted on Bio-spin disposable chromatography columns (Bio-Rad ...
-
bioRxiv - Biochemistry 2020Quote: Calmodulin (Gallus gallus) was recombinantly expressed from a pET-15b vector (generous gift from Angus Nairn) in BL21(DE3) cells (Millipore) and purified as previously described (40) ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were digested for at least 14 h (overnight) with an animal-free recombinant bovine trypsin expressed in corn (Trypzean T3568 from Sigma) at 1/50 w/w and RT ...
-
bioRxiv - Biochemistry 2021Quote: ... wild-type (WT) or D274N were individually expressed from a pET30-TEV vector in BL21 (DE3) pLysS Escherichia coli (Novagen), with protein expression being induced with 0.4 mM IPTG for 18 h at 18 °C ...
-
bioRxiv - Cell Biology 2021Quote: A Rab11/Ypt32 construct harbouring a GST tag at the N-terminus was expressed recombinantly in Rosetta2 cells (EMD Millipore) and purified using the procedure described previously (21) ...
-
bioRxiv - Molecular Biology 2021Quote: FLAG-tagged RPL3 was transiently expressed in METTL18 KO cells and immunopurified with ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich). The purified proteins were incubated in 1× reaction buffer (50 mM Tris-HCl pH 8.5 and 50 mM MgCl2 ...
-
bioRxiv - Biochemistry 2022Quote: ... All the constructs were expressed in Rosetta competent cells (F- ompT hsdSB(rB-mB-) gal dcm (DE3) pRARE (CamR)) (Novagen). To induce protein expression 0.5mM isopropyl B-D-thiogalactopyranoside was added to cells when they reached a density of 0.5-0.6 OD/ml ...
-
bioRxiv - Biochemistry 2022Quote: Streptococcus pyogenes Cas9 wild type protein and the nuclease dead mutant (D10A, H840A) were both recombinantly expressed for 16 hours at 18 °C in Escherichia coli Rosetta 2 (DE3) (Novagen) N-terminally fused to a hexahistidine affinity tag ...
-
bioRxiv - Biochemistry 2022Quote: Human histones cloned into pET3a (plasmids were a gift from Martin Browne and Andrew Flaus NUI Galway) were expressed separately in Rosetta2 (DE3) pLysS (Novagen). Cell pellets were resuspended in 30 ml histone wash buffer which contained 50 mM Tris/Cl pH 7.5 ...
-
bioRxiv - Neuroscience 2022Quote: ... Currents of 5-HT3A receptors and engineered constructs expressed at the cell surface were evoked by applying 10 μM serotonin creatinine sulfate complex (Sigma), or in short 5-HT ...
-
bioRxiv - Cell Biology 2022Quote: ... GBP and BicD2 constructs were bacterially expressed and grown in 800 mL of Terrific Broth (Sigma Aldrich, St. Louis, MO) at 37 °C until the OD = 1-2 ...
-
bioRxiv - Biochemistry 2023Quote: ... where Cue1(24-203) (pAS153) and Ubc7 (pAS159) were expressed with an N-terminal His6 tag in a pET28B vector (Novagen). Cue1 and Ubc7 were also expressed from a K27-His14-pSUMO vector allowing for the complete removal of affinity and solubility tags55 ...
-
bioRxiv - Plant Biology 2024Quote: ... Strep–NFR5CD–HA or Strep–NFR5CD–HA were co– expressed with NopT–FLAG or NopTC93S–FLAG by using Duet expressing vectors (Novagen) (Han et al. ...
-
bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
-
bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
-
bioRxiv - Biochemistry 2024Quote: ... was recombinantly expressed from a pET-15b vector (a gift from A. Nairn, Yale School of Medicine) in BL21(DE3) cells (Millipore) and purified as previously described (Stratton et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... Frozen samples were sent to Eve Technologies and analyzed using the Rat Cytokine/Chemokine Array 27-Plex (BioPlex 200 Cytokine Array Assay Kit Source, Millipore Milliplex; Calgary, AB, Canada).
-
bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...