Labshake search
Citations for Millipore Sigma :
1651 - 1700 of 4429 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... at 37°C with 180 rpm shaking or on Tryptic Soy Agar (TSA) plates (Sigma) at 37°C overnight ...
-
bioRxiv - Biochemistry 2024Quote: 1-2 μL from the reaction was spotted on a silica TLC plate (Millipore Sigma) and allowed to dry for a minimum of 15 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: HEK293T cells were seeded (2.5x106) on 10 cm plate coated with poly-L-lysine (Sigma) to obtain a confluence of 50-70% at the time of the infection ...
-
bioRxiv - Cell Biology 2024Quote: For total immunoglobulin levels in serum 96-well enzyme-linked immunosorbent assay plates (Sigma, CLS3590) were coated with 50 µg/mL capture antibody (Southern Biotech ...
-
bioRxiv - Cancer Biology 2024Quote: ... Plates were coated with 20 μg/ml laminin (Sigma, L2020 or Trevigen, 3446-005-01) for all lines except PBT-04 ...
-
bioRxiv - Cell Biology 2023Quote: ... MSA plates with or without 2.5 μg/mL of anhydrotetracycline (ahTet) (Sigma Aldrich, Cat#37919) were used59 ...
-
bioRxiv - Genetics 2020Quote: ... 200 µg/ml Proteinase K (Proteinase K, recombinant, PCR Grade, 3115879001, Sigma Aldrich)) ...
-
bioRxiv - Neuroscience 2021Quote: ... Diluted cDNA was amplified by PCR using the Sybr Green Master Mix (Sigma), and raw threshold-cycle time (Ct ...
-
bioRxiv - Molecular Biology 2021Quote: ... the TADA2b cDNA fragment was PCR amplified and cloned in pET15b vector (Novagen) using Nde I and Bam HI sites ...
-
bioRxiv - Cell Biology 2019Quote: ... The biotinylated probe used was generated by PCR using biotinylated-16-UTP (Sigma) and primers P3 and P4 ...
-
bioRxiv - Molecular Biology 2019Quote: ... but tested negative for mycoplasma by PCR-based detection kit (Sigma-Aldrich D9307). Medium containing 0.5mM palmitate was prepared by first dissolving FFA-free BSA (Fisher Scientific BP9704100 ...
-
bioRxiv - Biophysics 2019Quote: ... We visualized PCR products and DNA plasmids by 1 % agarose (SIGMA Cat.#A9539) gel dyed with GelRed (BIOTIUM Cat.#41003) ...
-
bioRxiv - Pathology 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035). iPSC lines for the iMg were cultured by the Human Pluripotent Stem Cell Core (CHOP).
-
bioRxiv - Immunology 2019Quote: ... Respective cDNA regions were PCR amplified and cloned into the pET24b vector (Novagen) for bacterial expression ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... We purified the PCR product using the GenElute Plasmid Miniprep Kit (Sigma Aldrich) and used it as a template to insert the mutation into OmpF— transformed with pkD46 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the gene encoding CAT was PCR-amplified from pACYC-Duet™-1 (Novagen) to include a 3’ flexible linker (GTSAAAG ...
-
bioRxiv - Immunology 2019Quote: ... Cells were verified as mycoplasma-free using the LookOut PCR detection Kit (Sigma).
-
bioRxiv - Bioengineering 2019Quote: PCR master mix: 1x KOD Xtreme Hot Start DNA Polymerase buffer (EMD Millipore), 1.2 µM (each ...
-
bioRxiv - Genomics 2020Quote: ... a specific FAM digital PCR assay was designed (Sigma-Aldrich, Gillingham, Dorset, UK) targeting the promotor region of RASSF1A and five 5’-CGCG-3’ BstUI recognition sequences ...
-
bioRxiv - Cell Biology 2020Quote: ... The PCR product designed for homologous recombination was transformed using lithium acetate (Sigma) as previously described (Tripp et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems, Sigma-aldrich, St. Louis MA). The sequencing libraries were clustered on a single lane of a flowcell ...
-
bioRxiv - Biochemistry 2021Quote: ... DNA fragments were PCR-amplified with KOD Hot Start Master Mix (Novagen®) from genomic DNA of M ...
-
bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
-
bioRxiv - Microbiology 2020Quote: ... Inverse PCR using this construct was carried out with KOD polymerase (EMD Millipore) according to manufacturers instructions ...
-
bioRxiv - Microbiology 2021Quote: PCR reactions were carried out with either KOD Hot Start DNA Polymerase (Sigma) or iProof DNA Polymerase (Bio-Rad) ...
-
bioRxiv - Microbiology 2021Quote: ... The amplified cDNA was purified with a GenElute PCR cleanup kit (Sigma-Aldrich), and concentration ...
-
bioRxiv - Cell Biology 2020Quote: ... Conventional PCR was performed using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The fragments were cloned into pCR-Blunt II TOPO using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... Quantitate PCR was conducted using KAPA SYBR FAST Master Mix (Sigma-Aldrich, KK4611) on a Roche Lightcycler 96 (Roche Life Science) ...
-
bioRxiv - Microbiology 2022Quote: ... The RNA was subsequently checked for DNA by PCR using RedTaq readymix (Sigma). RNA integrity was assessed using 2100 Bioanalyzer (Agilent ...
-
bioRxiv - Genomics 2022Quote: ... mm9) ampli?ed by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich, 11636090910). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... Nectin-3 alpha was PCR amplified (KOD hot start DNA polymerase, Sigma-Aldrich) from a mouse brain cDNA library using the forward primer ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... PCR product was digested with NcoI and XhoI and cloned into pET28a (Novagen) in frame with a C-terminal his-tag coding sequence E ...
-
bioRxiv - Cell Biology 2019Quote: ... PCR was performed using the KOD Hot Start DNA polymerase kit (Novagen, TOYOBO). The list of primers used for RT PCR is indicated on table 3.
-
Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2020Quote: ... Mycoplasma was not detected with the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2019Quote: ... with PCR primers with overhangs containing restriction sites for SpeI and NotI (Sigma). pLVX-EF1α-IRES-ZsGreen1 (Cat# 631982 ...
-
Orange is the new white: taxonomic revision of Antarctic Tritonia species (Gastropoda: Nudibranchia)bioRxiv - Zoology 2020Quote: ... 3.3 μL REDExtract-N-Amp PCR ReadyMix (Sigma Aldrich, St. Louis, MO, USA), 0.3 μL of each primer ...
-
bioRxiv - Molecular Biology 2021Quote: ... bL33 were PCR-amplified and cloned individually into the pET24(a) plasmid (Novagen). Similarly ...
-
bioRxiv - Genomics 2019Quote: ... The libraries were PCR amplified with KAPA HiFi Hotstart Ready mix (Sigma-Aldrich) and barcoded Nextera custom primers 17 and finally size-selected (250–350 bp ...
-
bioRxiv - Biochemistry 2021Quote: ... The purified PCR products were concentrated using Amicon Ultra centrifugal filter units (Millipore) and buffer exchanged against 10 mM HEPES pH 7.0 ...
-
bioRxiv - Neuroscience 2019Quote: ... Lines were tested for mycoplasma using Lookout mycoplasma PCR detection kit (Sigma MP0035).
-
bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit (Sigma): 50 µl of extraction solution and 12.5 µl of tissue preparation solution was added to each embryo ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were visualized after agarose gel electrophoresis using GelRed® stain (Sigma) and a GelDoc imaging system (Bio-Rad).
-
bioRxiv - Biochemistry 2020Quote: All PCR reactions were carried out using KOD Hot Start DNA polymerase (Novagen). All RT-PCRs were carried out using Takara’s PrimeScript High Fidelity RT-PCR kit (R022A).
-
bioRxiv - Molecular Biology 2020Quote: ... and PCR on extracted DNA (GenElute Mammalian Genomic DNA Miniprep Kit; Sigma-Aldrich). Selected clones were finally adapted to growth in suspension in Expi293 Expression Medium (Thermo Fisher Scientific).
-
bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
-
bioRxiv - Genetics 2020Quote: ... Cell lines were tested for mycoplasma contamination using the Mycoplasma PCR ELISA (Sigma).
-
bioRxiv - Cell Biology 2021Quote: ... Mycoplasma contaminations were tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The amplified cDNA was purified with the GenElute PCR cleanup kit (Sigma-Aldrich), and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies) ...
-
bioRxiv - Cancer Biology 2022Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, USA). LNCaP abl were subjected to short tandem repeat (STR ...