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Citations for Millipore Sigma :
1901 - 1950 of 4429 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... samples were filtered (MultiScreen® filter plates with 0.45 μm Durapore® membranes; Merck Millipore, Germany) and measured using HPLC (Dionex Ultimate 3000 ...
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bioRxiv - Microbiology 2024Quote: ... cells were streaked onto LB agar plates supplemented with 100 µM 2-methylbutyric acid (MB) (Sigma), and grown at 37 °C ...
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bioRxiv - Microbiology 2024Quote: 9 μl of total mycobacterial lipids were spotted onto aluminum-backed silica TLC plates (Millipore Sigma) and dried ...
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bioRxiv - Immunology 2024Quote: ... Water from Milli-Q Advantage A10 and MultiScreen-HV 96-well Plates were used (Merck Millipore). Calibration ranges were 3.125 – 200 ng/mL for U and 25 - 500 ng/mL for UH2 and 5-FU (5µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... jejuni was resuspended from plates into either control or 1.0 mM TMAO in MEME medium (Sigma), supplemented with 2 mM L-Glutamine (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... These animals were then transferred to 6cm low peptone plates with added 15mM serotonin (Sigma Aldrich) for 10 mins ...
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bioRxiv - Microbiology 2024Quote: Overnight cultures from each master plate were grown in Mueller-Hinton broth (MH) (Sigma-Aldrich, UK) and diluted 1:100 in MH ...
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bioRxiv - Molecular Biology 2024Quote: ... transduced cells were expanded in 6-well plates with complete medium containing 10 μM TMP (Sigma), cultured for 48 hours ...
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bioRxiv - Immunology 2024Quote: ... The plate was spun down and washed once with FACS buffer (0.5% BSA in PBS; Sigma), then resuspended in 100ul of FACS buffer.
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bioRxiv - Molecular Biology 2024Quote: ... 5% CO2 in a humidified incubator on tissue-culture plates covered by 0.2% gelatin (Sigma, G1890) in 2i medium with LIF ...
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bioRxiv - Cancer Biology 2024Quote: ... Seahorse 96XFe tissue culture plates were coated with 0.01% poly-L-ornithine solution (Sigma-Aldrich, #P4957) and incubated at 37°C ...
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bioRxiv - Microbiology 2024Quote: Bacteria from lung homogenates were enumerated by plating on cetrimide + 1 % glycerol agar plates (Sigma-Aldrich) and incubated overnight in 37°C ...
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bioRxiv - Immunology 2024Quote: ... Plates were finally developed using o-phenylenediamine (OPD) tablets mixed with sodium perborate buffer (Sigma-Aldrich). All plates were read on a MultiSkan Sky ELISA plate reader (ThermoFisher Scientific MA USA ...
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bioRxiv - Cancer Biology 2024Quote: 12-well cell culture plates were coated with 50 µg/ml poly-D-lysine (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... and verified to be mycoplasma-free using LookOut® Mycoplasma PCR Detection Kit (Sigma). Density gradient purified Chlamydia trachomatis L2/434/Bu (ATCC VR902B ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR fragments generated were cloned into a pET-41 Ek/LIC vector (Novagen) expressing an N-terminally GST-His6-tagged protein ...
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bioRxiv - Microbiology 2020Quote: ... The linear constructs were gel purified using a PCR cleanup kit (Sigma-Aldrich, Australia), confirmed by Sanger sequencing and concentrated to ∼500 ng/μl.
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bioRxiv - Microbiology 2021Quote: ... which was combined with 10µL of KiCqStart SYBR Green PCR Ready Mix (Millipore Sigma), 1µL of forward primer ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... Q-PCR was performed with SYBR Green Jump-start Taq Ready-mix (Sigma, S4438) on the Mx3005P qPCR System (Agilent Technologies Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were tested for mycoplasma using the LookOut mycoplasma PCR detection kit (Sigma MP0035), following the manufacturer’s instructions and using the JumpStart Taq DNA polymerase
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bioRxiv - Neuroscience 2019Quote: ... The wild-type or T94M mutant ORFs were then PCR-cloned into pET28c (Novagen) for bacterial production of the proteins with an N-terminal 6× His tag ...
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bioRxiv - Cancer Biology 2021Quote: ... The PTBP1 ORF was PCR amplified and subcloned by gibson cloning into pET21A+ (Novagen) digested by XhoI and NheI ...
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bioRxiv - Biochemistry 2021Quote: ... PCR products were separated by electrophoresis on a 2.5% agarose gel containing GelRed (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The resulting PCR fragment and the Plasmid DNA of the overexpression vector pET28a(+) (Novagen) were digested with NdeI and BamHI ...
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bioRxiv - Genomics 2020Quote: ... Cultures were routinely tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, USA) to confirm negative infection status.
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bioRxiv - Microbiology 2019Quote: ... The digests were then purified with either GenElute PCR Clean-Up Kit (Sigma-Aldrich) or QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma contamination was tested monthly using the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). All cells were mycoplasma-free ...
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bioRxiv - Microbiology 2021Quote: ... DNA from tail tips was isolated using Extract-N-Amp Tissue PCR kit (Sigma) and PCR genotyping undertaken as described (The Jackson Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with a commercial kit (Sigma JumpStart™ Taq ReadyMix™, P2893) and 1-μL of purified DNA ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed using the SYBR Green JumpStart Taq Ready Mix (Sigma Aldrich) on an ABI Prism 7900 system (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2020Quote: ... All PCR reactions were carried out by KOD Hot Start DNA polymerase (Merck Millipore). PCR and Gel purification were done using Qiaquick nucleotide removal kit (qiagen ...
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bioRxiv - Genomics 2020Quote: ... we added 11 µl of KAPA2G Robust PCR master-mix (Sigma Catalogue No. KK5005), comprising 4 µl KAPA2G buffer ...
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bioRxiv - Microbiology 2020Quote: ... A second round of PCR performed with KOD Hot Start Master Mix (Millipore, 71842) supplemented with 1mM MgCl2 under the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... All inverse PCR reactions were carried out using KOD hot-start polymerase (EMD Millipore) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested negative for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma, MP0035). All cells were maintained at 37°C in a humidified incubator with 5% CO2 and were grown in media supplemented with 10% dialyzed fetal bovine serum (Sigma-Aldrich F0392) ...
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bioRxiv - Paleontology 2019Quote: ... Bisulfite treated DNA were amplified with the FastStart High Fidelity PCR System (Sigma, 03553400001) using the primers listed in Extended Data Table 12 ...
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bioRxiv - Cell Biology 2021Quote: ... qRT-PCR was performed with Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07; Sigma). Inventoried TaqMan probes for qRT-PCR from Applied Biosystems were used for human TULP3 and GAPDH ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealed fragments were purified using the GenEluteTM PCR Clean-Up Kit (Sigma Aldrich, NA1020). Bi-directional precursor construct (pZ073 ...
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bioRxiv - Immunology 2021Quote: ... PCR amplicons were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). The size of barcoded PCR products (approximately 1500 bp ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Table S6 ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were sub-cloned into the plasmid expression vector pET-28a (Novagen) previously cut with the restriction enzymes EcoR I and Xho I ...
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bioRxiv - Bioengineering 2022Quote: ... and (ii) inlets of the hydrogel chamber were sealed using PCR tape (Sigma Aldrich). Depending on the type of analysis ...
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bioRxiv - Biochemistry 2022Quote: ... The sequence was verified by colony PCR using standard primers (Sigma-Aldrich, Taufkirchen, Germany) and Taq polymerase (In house ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was detected by electrophoresis on agarose gel (Sigma-Aldrich, Darmstadt, Germany), using ethidium bromide (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR was carried out using SYBR Green (Jumpstart Taq ReadyMix: Sigma-Aldrich) in 10 μl volumes for 3 biological and 3 technical replicates with 1:10 diluted cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were transformed into pKM208 carrying Rosseta 2 (Millipore Sigma 71400-3) strain by electroporation ...
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bioRxiv - Immunology 2022Quote: ... ORFs were synthesized as gBlocks (IDT) and assembled using PCR (KOD mastermix, Milipore Sigma) using the following primers ...
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bioRxiv - Neuroscience 2023Quote: Fragments of eaat genes were PCR amplified using a JumpStart Taq polymerase (Sigma-Aldrich) and the primer pairs eaat5b_135_s 5’-GGAGCAGGAAGTCAAGTA-3’ with eaat5b_600_as 5’-GTCCGTCCCATTATCGTC-3’ and eaat7_142_s 5’-GTAATAGCAGGCACAGTGATG-3’ with eaat7_1001_as 5’-CCCAGAGCAGTGATCCAAG-3’ ...