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Citations for Millipore Sigma :
1601 - 1650 of 4702 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and PCR on extracted DNA (GenElute Mammalian Genomic DNA Miniprep Kit; Sigma-Aldrich). Selected clones were finally adapted to growth in suspension in Expi293 Expression Medium (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
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bioRxiv - Genetics 2020Quote: ... Cell lines were tested for mycoplasma contamination using the Mycoplasma PCR ELISA (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... Mycoplasma contaminations were tested using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The amplified cDNA was purified with the GenElute PCR cleanup kit (Sigma-Aldrich), and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, USA). LNCaP abl were subjected to short tandem repeat (STR ...
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bioRxiv - Bioengineering 2022Quote: ... After digestion the PCR product was ligated into the pET30a vector (EMD Millipore). The resulting construct encodes EcSTH with both an N-terminal Hisx6-tag and a C-terminal FLAG-tag.
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... It was generated by PCR amplification (DNA KOD Hot-start DNA Polymerase, Novagen) and cloned into the pENTRD-TOPO Gateway vector using the manufacturer’s protocol and confirmed by sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were performed with KOD Hot Start DNA Polymerase (Sigma 71086-3) using a Mastercycler X50a (Eppendorf) ...
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bioRxiv - Biophysics 2024Quote: ... sample wells (Fig. S14) were made from half-cut PCR tubes (Sigma-Aldrich) glued to the prepared slides with epoxy (Ellsworth Adhesives) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were tested for mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich).
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme, Merck Millipore) according to manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: PCR reactions were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) under the following conditions ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
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bioRxiv - Physiology 2023Quote: ... DNA extraction was done using REDExtract-N-Amp Tissue PCR kit (XNAT, Sigma). For the PCR reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was subcloned and inserted into a pET-24b (+) plasmid (Novagen) digested with NheI/XhoI placing the gene upstream of a sequence encoding six histidine residues ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was performed using FastStart Universal SYBR Green Master Mix (Sigma, 4913850001). The aggregates of three housekeeping genes (B2m ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using SYBR green PCR Jumpstart Taq ReadyMix (Sigma-Aldrich), ROX Reference Dye (ThermoFisher Scientific ...
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Extrusion fountains are hallmarks of chromosome organization emerging upon zygotic genome activationbioRxiv - Molecular Biology 2023Quote: ... Sanger sequencing of the PCR products was performed using a commercial service (Sigma). The sequencing traces were examined and the fish carrying A to G mutation were selected ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were screened for mycoplasm using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) or Mycostrip detection kit (Invivogen) ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed using Jumpstart SYBR green mastermix (Sigma-Aldrich) on a Rotorgene 6000 (Corbett Research) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplification was done by using Taq DNA polymerase (Sigma-Aldrich®, USA), and the PCR product was digested with the restriction enzymes shown in Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... The reaction mix was: 0.45 µL DMSO for PCR (Sigma; cat# D8418-50mL), 0.0045 µL SYBR Green I (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene rpsU was kept as an endogenous control ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We carried out polymerase chain reactions (PCRs) with Hot Start polymerase (MERCK MILLIPORE) and performed colony PCRs with Taq polymerase (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated lipids were spotted onto heated glass-backed silica gel 60 plates (Millipore Sigma 1057210001), and neutral lipids were separated in a mobile phase of 80:20:1 hexane:diethyl ether:glacial acetic acid ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed (5 times with PBS-T) and developed with either AP substrate (Sigma) and read at 405 nm (AP ...
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bioRxiv - Immunology 2021Quote: ... An additional plate was coated with goat anti-human IgG (Sigma, cat. no. I2136-1ML) at 4 μg/ml and IgM (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Prior to cell seeding the plates were treated with 0.01% Poly-L-Ornithine (Millipore, A004C) for at least 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were further cultured on plates coated with 7.5 μg/ml Fibronectin (Sigma, cat. F1141) in EpiSCs medium ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were further cultured on plates coated with 7.5 μg/ml Fibronectin (Sigma, cat. F1141) in the same medium ...
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bioRxiv - Developmental Biology 2020Quote: ... 20 day-one adult animals were transferred onto NGM plates containing 1 mM aldicarb (Sigma) without food ...
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bioRxiv - Developmental Biology 2020Quote: ... 100µL transformation mix was spread on an Agar plate containing 100µg/mL Ampicillin (A5354, Sigma) and incubated over night at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Immediately prior to plating on agar plates containing 20 mM 4-chloro-phenylalanine (Sigma-Aldrich), the cells were washed twice with phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... plates or dishes were coated with 10 µg/ml Poly-L-Ornithine (PLO, Sigma, P4957) in PBS for at least 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... We used plates containing either 0.5 mM or 1 mM final aldicarb (33386 Sigma-Aldrich) concentration in the NGM ...
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bioRxiv - Immunology 2021Quote: ... plates were washed and incubated for 30 minutes with 1μg/ml Streptavidin-Alkaline Phosphatase (Sigma). Finally plates were washed and developed with BCIP®/NBT (Sigma).
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bioRxiv - Immunology 2021Quote: ... The plates were then washed 3 times with PBS containing 0.05% Tween-20 (Sigma-Aldrich), blocked with 150 mL ELISA/ELISPOT Diluent (Life Technologies Corporation ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 × 103 cells were seeded into 96-well plates and stained with MTT (Sigma-Aldrich) dye ...
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bioRxiv - Physiology 2019Quote: ... on LB Agar Bacterial resistant plates (50 μg/ml Spectinomycin Sigma Aldrich cat no. S4014) in SOC medium (Invitrogen cat no ...
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bioRxiv - Synthetic Biology 2020Quote: ... Spores were harvested from plates in 1 mL of 0.1% Tween 80 (Sigma, MO, USA) and after counting under Neubauer chamber ...
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bioRxiv - Developmental Biology 2019Quote: ... Injected animals were grown on NGM plates supplemented with 1 mM auxin (Sigma-Aldrich, I2886). Injection mixes contained pCFJ601 (Peft-3∷transposase ...
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bioRxiv - Cell Biology 2020Quote: ... 7 μL sample was spotted on a polyethylenimine (PEI) cellulose TLC plate (Sigma-Aldrich, Z122882), and resolved using 0.75 M LiCl ...
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bioRxiv - Microbiology 2019Quote: ... The transformed cells were selected on plates containing 300 mg/L Hygromycin B (Sigma-Aldrich). Colony PCR using primer pairs 1055F/2951R and STA1_UAS_Fw/STA1_UAS_Rv ...
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bioRxiv - Cancer Biology 2019Quote: ... Each plate was transfected with 20 μg of overexpression plasmid with 100 μg PEI (Sigma). After 48 h cells were collected in TBS ...
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A tyrosine kinase protein interaction map reveals targetable EGFR network oncogenesis in lung cancerbioRxiv - Cancer Biology 2020Quote: ... Cells were reverse transfected in quadruplicate in 384-well plates with 20nM of siRNA (Sigma) using RNAiMax as transfection reagent ...
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bioRxiv - Microbiology 2021Quote: ... Environmental water aliquots were also plated on LB agar plates (Sigma-Aldrich, St. Louis, MO) to estimate live bacteria quantities prior to freezing at −80°C.