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Citations for Millipore Sigma :
1701 - 1750 of 4429 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... After digestion the PCR product was ligated into the pET30a vector (EMD Millipore). The resulting construct encodes EcSTH with both an N-terminal Hisx6-tag and a C-terminal FLAG-tag.
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... It was generated by PCR amplification (DNA KOD Hot-start DNA Polymerase, Novagen) and cloned into the pENTRD-TOPO Gateway vector using the manufacturer’s protocol and confirmed by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were tested for mycoplasma using LookOut Mycoplasma PCR Detection Kit (Sigma Aldrich).
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bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme, Merck Millipore) according to manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: PCR reactions were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) under the following conditions ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
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bioRxiv - Physiology 2023Quote: ... DNA extraction was done using REDExtract-N-Amp Tissue PCR kit (XNAT, Sigma). For the PCR reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR product was subcloned and inserted into a pET-24b (+) plasmid (Novagen) digested with NheI/XhoI placing the gene upstream of a sequence encoding six histidine residues ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was performed using FastStart Universal SYBR Green Master Mix (Sigma, 4913850001). The aggregates of three housekeeping genes (B2m ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... qPCR reactions were prepared using SYBR green PCR Jumpstart Taq ReadyMix (Sigma-Aldrich), ROX Reference Dye (ThermoFisher Scientific ...
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Extrusion fountains are hallmarks of chromosome organization emerging upon zygotic genome activationbioRxiv - Molecular Biology 2023Quote: ... Sanger sequencing of the PCR products was performed using a commercial service (Sigma). The sequencing traces were examined and the fish carrying A to G mutation were selected ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were screened for mycoplasm using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich) or Mycostrip detection kit (Invivogen) ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed using Jumpstart SYBR green mastermix (Sigma-Aldrich) on a Rotorgene 6000 (Corbett Research) ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mix was: 0.45 µL DMSO for PCR (Sigma; cat# D8418-50mL), 0.0045 µL SYBR Green I (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We carried out polymerase chain reactions (PCRs) with Hot Start polymerase (MERCK MILLIPORE) and performed colony PCRs with Taq polymerase (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were performed with KOD Hot Start DNA Polymerase (Sigma 71086-3) using a Mastercycler X50a (Eppendorf) ...
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bioRxiv - Biophysics 2024Quote: ... sample wells (Fig. S14) were made from half-cut PCR tubes (Sigma-Aldrich) glued to the prepared slides with epoxy (Ellsworth Adhesives) ...
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bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... and positive colonies were selected on LB plates containing spectinomycin (100ug/mL, Sigma-Aldrich, Catalog # 85555). The construct was sequence verified (Eton Bioscience ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was run on 1D-thin layer chromatography on cellulose plates (TLC, 20 cm, Merck Millipore) with solvent (isopropanol/HCl/water (70/15/15) ...
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bioRxiv - Immunology 2021Quote: ... a concentration of 1×105 cells per mL were seeded into 96 well plate (Sigma Aldrich). After 24h ...
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bioRxiv - Cancer Biology 2019Quote: ... NSCs were maintained in serum-free adherent growth conditions on poly-L-lysine-coated plates (Sigma). 1µg/ml Laminin (Sigma ...
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bioRxiv - Genetics 2021Quote: ... At L4 larval stage animals were transferred to plates containing 10 µM of 5-Fluorouracil (Sigma) in order to prevent progeny production ...
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bioRxiv - Microbiology 2020Quote: ... coli DH10B pks− were seeded in a 96-well plate in LB liquid medium (Sigma-Aldrich). The optical density (OD ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the plastic tissue culture-treated plates were coated with 0.1% gelatin (EMD Millipore ES-006-B) at room temperature for at least 30min ...
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bioRxiv - Immunology 2021Quote: ... Rat splenocytes (2 × 105) were plated in duplicate in 96-well PVDF plates (Millipore, Billerica, MA) and stimulated with 5 μg/ml of recombinant 2019-nCoV Spike protein (S1+S2 ECD ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were seeded in 96-well plates pretreated with fibronectin (10µg/ml 60 min; Sigma-Aldrich) at 3.5 × 104 cells /well ...
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bioRxiv - Neuroscience 2021Quote: ... Nunclon flasks and plates were treated with a 44 µg/ml Poly-L-Ornithine (Sigma P3655) and 1 µg/ml fibronectin (Sigma F1141 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl were spotted onto plates with or without 100 μg/ml terbinafine (Sigma-Aldrich). Plates were imaged after 3–7 days of incubation at 30°C.
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bioRxiv - Molecular Biology 2020Quote: ... Seeds were surface sterilized and grown on plates with 1/2MS Basal Salt Mixture (M5524: Sigma), MES (0.05% ...
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bioRxiv - Cell Biology 2022Quote: ... 20 worms were placed on solid NGM plates containing 0.4M paraquat (PESTANAL, Sigma-Aldrich, MO, USA) for 3 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA from each plate was extracted using the GenEluteTM Mammalian Genome DNA miniprep Kit (Sigma). DNA fragments containing transposon/genome junctions were amplified via ligation mediated PCR and sequenced using the Illumina Hi-Seq 4000 platform as previously described (22).
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bioRxiv - Immunology 2019Quote: ... the plates were incubated with 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (A3219; Sigma-Aldrich) at room temperature for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... a 96-well plate pre-coated with a variety of ECM proteins was used (EMD Millipore; Collagen I ...
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bioRxiv - Bioengineering 2019Quote: ... One plate of the cells was treated with 10−8 M 17β-estradiol (E8875, Sigma-Aldrich) and the other plate of the cells was treated with DMSO at the same concentration ...
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Cell Biology 2019Quote: ... For levamisole and aldicarb paralysis assays NGM plates were prepared with 0.2 mM levamisole (Sigma #T1512) and 1 mM aldicarb (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... The indicated volume of sample was spotted onto TLC silica gel 60 T254 plates (Merck Millipore) and resolved using a mixture of 60 chloroform ...
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bioRxiv - Molecular Biology 2021Quote: 2 µl of the digested RNA is spotted on PEI-cellulose TLC plates (Millipore Sigma #105579) 0.5 µl at a time ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.05 MgSO4 per liter of deionized water) agar plates containing Nile red (Sigma-Aldrich, Missouri, USA) at 0.5 mg per liter ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with washing buffer (1× PBS with 0.05% Tween-20 (Sigma-Aldrich)) and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... animals were directly grown on culture plates containing 12.8 nM adenosylcobalamin (adoCbl) or methylcobalamin (meCbl) (Sigma). For methionine supplementation ...
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bioRxiv - Biochemistry 2020Quote: ... All experiments were done in plates coated with poly-DL-ornithine (Sigma-Aldrich, St. Louis, MO). Cell lines were authenticated by Hybribio (Guangzhou ...
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bioRxiv - Bioengineering 2020Quote: ... The cell suspension was transferred into Ultra-Low Attachment (ULA) 96-well round-bottom plates (Sigma). Cell suspension of 200 µl was loaded in each well of the 96-well plate ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2, Sigma Aldrich). Subsequently ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were fixed using 4% paraformaldehyde for 15 mins and stained with crystal violet (0.5%, SIGMA).
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bioRxiv - Cancer Biology 2020Quote: ... Plates were washed under running water and cells were stained with 1mL sulforhodamine B (Sigma 230162) and incubated at room temperature for 30 minutes ...
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bioRxiv - Physiology 2021Quote: ... and plated in 6-well plates (0.25×106 cells per well) in William’s Medium E (Sigma) supplemented with antibiotics and 5% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... the concentrated urinary proteomes were quantified via a 96 well plate Bradford protein assay (Sigma-Aldrich) using and Bovine Serum Albumin to generate the calibration curve.