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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The aorta was cut into fragments of about 1-2mm and digested for 2 hours in an enzyme solution containing collagenase type II (3mg/mL; C6885, 700.3U/mg, Sigma). and elastase (1mg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... and blocked for 2 hrs at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma). Following ...
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bioRxiv - Cancer Biology 2023Quote: Culture medium was replaced with 100 µL medium containing 5 mg/mL filtered 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (#M5655, Sigma-Aldrich) and incubated for 2 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and then transferred to maturation media (DMEM containing low glucose (2 g/L) with Palmitic acid (200 uM, Sigma), Dexamethasone (100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended in 150 µl of FACS buffer (PBS supplemented with 0.5 % fetal bovine serum and 2 mM EDTA) containing DAPI (1:50,000, Sigma-Aldrich) as previously described (Fischer et al ...
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bioRxiv - Microbiology 2023Quote: Mtb WT and virRmut strains were grown in 7H9 media supplemented with ADC and Tween 80 to an OD 540 of 0.2 and then diluted to an OD540 of 0.1 with 7H9 containing 0 or 50 µgml-1 lysozyme (Sigma). The cultures were incubated with agitation at 37°C and OD540 was monitored daily.
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bioRxiv - Neuroscience 2023Quote: ... Sections were permeabilized with 0.25% Triton X-100 in PBS and then blocked with PBST containing 2% bovine serum albumin (Sigma). Sections were incubated at 4°C overnight with biotinylated HA-binding protein (b-HABP ...
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bioRxiv - Bioengineering 2023Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Single cells of two untreated CeD patients were sorted into 96-well plates containing 2 µl of scRNA-seq catch buffer (0.2% vol/vol Triton X-100 [Sigma] in H2O with 2 U/μl RNase inhibitor [New England Biolabs] ...
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bioRxiv - Bioengineering 2023Quote: ... was weighted and placed into a tube containing 2 mL 0.2% v/v Triton X-100 (108603, Sigma-Aldrich) and 5 mM MgCl2 (M2393 ...
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bioRxiv - Bioengineering 2023Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were fixed with 2% paraformaldehyde (PFA) and permeabilized with PBS containing 0.2% Triton-X-100 (Sigma-Aldrich). Cells were blocked for one hour at room temperature with PBS containing 0.05% Triton-X and 5% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM Glucose) containing 1µg/mL of 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biochemistry 2024Quote: ... homogenised in 6 mL of 2:1 (v/v) chloroform:methanol containing 0.01% (w/v) butylated hydroxytoluene (BHT, Sigma-Aldrich) as antioxidant ...
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bioRxiv - Bioengineering 2024Quote: ... blocked for 2 hr at room temperature in PBSTX (DPBS + 0.1% Triton X-100) containing 5% donkey serum (D9663, Sigma), incubated in primary and then secondary antibodies in blocking buffer for at least 48 hr at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Embryos were transferred to E3 solution containing 5 µg/ml proteases and 0.2 mM1-phenyl-2-thio-urea (Sigma) 24 h post-fertilization to dechorionate the fish embryos and prevent pigmentation ...
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bioRxiv - Molecular Biology 2021Quote: ... the stained band containing the protein sample was excised and de-stained by washing with 50 mM ammonium bicarbonate (Sigma) and 100% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were then washed 4x with Buffer A containing only 0.1% NP-40 and bound protein was eluted by 3 sequential incubations in PBS containing 0.1% triton x-100 (Sigma-Aldrich) and 0.5mg/ml of 3x FLAG peptide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then transferred onto polyvinylidene fluoride membrane (PVDF, Hybond-P, Amersham) in transfer buffer containing 12 mM Tris (Sigma), 96 mM glycine (BDH ...
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bioRxiv - Molecular Biology 2019Quote: ... A volume of 12.5 μl of a solution containing 500 ng protein was diluted in H2O with 5× Sypro Orange (Sigma-Aldrich) and immediately mixed with an equal volume of assay condition ...
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bioRxiv - Neuroscience 2019Quote: Total protein was extracted from L4/5 DRGs or retinas using the RIPA buffer containing protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Samples of the eluted fractions were loaded into 15% SDS-PAGE gels and the fractions containing the nucleocapsid protein were concentrated using Amicon Ultra-15 concentric filters (Merck Millipore) with a 10 kDa cutoff ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing >90% pure protein (evaluated by SDS-PAGE) were concentrated using an Amicon Ultra-15 centrifugal unit (EMD Millipore) and flash frozen in liquid nitrogen before storage at −80°C.
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The resin was loaded into a column and the flow through containing S-tagged gyrase A was further purified using an S-protein agarose (Sigma) by elution with 20 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... a total of 4 μg of S protein diluted in 12 μl sterile ddH2O containing hyaluronidase (100 UI; Sigma-Aldrich) were infused in the intranasal cavity of mice ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the protein were pooled and concentrated with an Amicon Ultracell Centrifugal filter unit (MWCO 50 kDa, Merck Millipore) to a volume of 500 µL and digested with TEV protease for 2 hours at 8°C ...
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bioRxiv - Microbiology 2020Quote: Fractions containing the DnaA-6xHis protein were pooled together and applied to Amicon Ultra Centrifugal Filters with 30 kDa cutoff (Millipore). Buffer was exchanged to Buffer A (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... treated cells were washed with PBS and collected into RIPA buffer (Fujifilm Wako Pure Chemical) containing protein inhibitor (Sigma-Aldrich). The cells were incubated on ice for 30 min and then homogenized ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... The fractions of the flow-through containing MSP protein were pooled together and concentrated with a 10 kDa Amicon Ultra-filter (Millipore) to 100-200 μM ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant GST fusion proteins were eluted by resuspending the resin in elution buffer containing 15 mg/mL reduced L-glutathione (Sigma) and incubated at 4°C for 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... Protein was buffer exchanged into phosphate buffered saline (PBS) containing 10% glycerol using a spin concentrator (10 kDa MWCO, Millipore).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... aliquots of 15 µg of hippocampal protein were extracted with lysis buffer containing phosphatase and protease inhibitors (Cocktail II, Sigma) and protein concentration was determined by Bradford’s method ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Systems Biology 2020Quote: ... Total proteins from diverse organelles were extracted using a phase-transfer surfactant solution containing 12 mM sodium deoxycholate (Sigma–Aldrich), 12 mM sodium lauroyl sarcosine (MP Biomedical ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were eluted using 500 μl of IP Buffer containing 0.25 mg/ml of the 3×Flag peptide (Sigma-Aldrich) and 1× cOmplete EDTA-free for 15 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was normalized for all tissues with RIPA buffer and 2x SDS sample buffer containing β-mercaptoethanol (Sigma-Aldrich) was added ...
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bioRxiv - Genomics 2021Quote: ... 5 mM sodium metabisulfite and 1 mM MgCl2) containing 100 μg of purified protein and 100 μM GGPP (Sigma-Aldrich). The reaction mixture was overlaid with 500 μL of pentane (Macklin ...
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bioRxiv - Microbiology 2021Quote: ... Each animal received a single dose of 20 μg protein immunogen in 100 μl of PBS containing 50 μl of Sigma Adjuvant System (Sigma) with the procedure of immunogen and adjuvant mixture following manufacture’s manual ...
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bioRxiv - Immunology 2021Quote: ... Fractions containing the proteins were pooled and concentrated to ~ 8 mg/mL each with 30 kDa cutoff ultrafiltration units (Millipore). Time-resolved fluorescence resonance energy transfer (TR-FRET ...
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bioRxiv - Immunology 2020Quote: ... The protein was eluted with PBS containing 300 mM imidazole and 0.1% (v/v) protease inhibitor cocktail (Sigma, P-8849). For the CR3022 antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... the column was washed with 10 CV of binding buffer A and then the protein was eluted in binding buffer A containing 2.5 mM d-Desthiobiotin (Sigma-Aldrich). The eluted fractions were further purified using a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... or drug free tissue homogenate and extraction was performed by adding 200 µL of acetonitrile/methanol 50/50 protein precipitation solvent containing 10 ng/mL Verapamil (Sigma). Extracts were vortexed for 5 minutes and centrifuged at 4000 RPM for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were diluted to equivalent volumes containing 20 μg of protein and boiled in an equal volume of Laemli SDS boiling buffer (Sigma) for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... The first elution peak containing both proteins were pooled and concentrated to 10-15 mg/ml using Amicon Ultra Centrifugal Filter Units (Millipore) with 10-kDa molecular weight cutoffs.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with a solution containing the membrane pore-forming protein Streptoylsin O (SLO) (Sigma catalog: S5265-25KU) sufficient to permeabilize 90% of HeLa cells ...
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bioRxiv - Microbiology 2023Quote: ... a molecular ‘anchor’ was added to cellular proteins by incubating the coverslips in a solution containing 1.4% Formaldehyde (Sigma F8775) and 2% Acrylamide (Sigma A4058 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the soluble fractions containing the FLAG-tagged KDM2A protein were affinity purified using the anti-FLAG M2 affinity gel (Sigma). Gel beads were washed with TBS buffer (50 mM Tris ...