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Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation was achieved by incubating cell lysates containing 1 mg of protein overnight with anti-HA agarose beads (Sigma-Aldrich), Myc-agarose beads (Santa cruz biotechnology ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... the DuolinkTM InSitu Orange Starter Kit Mouse/Rabbit (DUO92102) from Sigma Aldrich was used ...
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using GFP primers ...
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bioRxiv - Cancer Biology 2021Quote: ... The rat/mouse insulin kit (EZRMI-13K) was purchased from EMD Millipore Corporation ...
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using primers listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2020Quote: The DuoLink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) was used to detect proximity between FXR1 protein and cMYC mRNA according to manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... using Milliplex Mouse Cytokine/Chemokine MAGNETIC BEAD Premixed 25 Plex Kit (Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and mouse insulin RIA kit (Cat#SRI-13K, Millipore, Billerica, Massachusetts, USA) was used to measure plasma insulin ...
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bioRxiv - Physiology 2020Quote: ... we performed PLAs using the Duolink® Starter Kit Mouse/Rabbit (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: The DNA construct coding for the motor domain of human kinesin-5 (Eg5; residues 1–368) was cloned into a modified pET16b (Novagen) vector for bacterial expression to achieve an N-terminally 6x His tag and TEV cleavage sequence ...
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bioRxiv - Biochemistry 2020Quote: A synthetic DNA fragment encoding the MTase domain of Arabidopsis thaliana DRM2 (residues 270-626) was cloned into pRSFDuet-1 vector (Novagen), preceded by an N-terminal His6-SUMO tag ...
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bioRxiv - Biochemistry 2022Quote: ... The protein complexes were eluted by on-column cleavage of MyoB neck domain using TEV and subsequently concentrated using a centrifugal concentrator (Millipore). The samples were analyzed using SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were diluted to a final concentration of 50 mM NaCl and then concentrated on Amicon centrifugal filters 30K for FHL and 10K for LIM domains (Millipore). The proteins’ purities and identities were confirmed by SDS-PAGE and mass spectrometry ...
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bioRxiv - Biochemistry 2021Quote: ... and 25 ng/μl E3 (HUWE1, catalytic domain, aa 3228-4374/1147 aa) were incubated with 0.3 μg/μl Ub (Sigma-Aldrich) and 11 ng/μl H1.2/H1.2 KxAc in 2 mM ATP ...
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bioRxiv - Biochemistry 2022Quote: The Sso2 sequence excluding only its C-terminal transmembrane domain (aa1-270) and the Sec3 PH domain sequence (aa75-260) were both sub-cloned into the pET-15b vector (Novagen) between the NdeI and BamHI sites ...
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bioRxiv - Neuroscience 2022Quote: ... we added polyclonal antibodies against the extracellular domain of TrkB (1:50 rabbit anti-TrkB, Millipore, cat no. AB9872, RRID:AB_2236301) together with 20 ng/mL BDNF for 2.5 hours ...
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bioRxiv - Biophysics 2020Quote: ... SEPT8G and SEPT11G) were performed by inserting the CDS for the corresponding G domains into the bicistronic expression vector pETDuet-1 (Novagen). The G domain of SEPT7 (SEPT7G ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μg/ml anti-E-cadherin blocking antibody that targeted the extracellular domain of E-cadherin (Gumbiner, Stevenson and Grimaldi, 1988) (uvomorulin, monoclonal anti-E-cadherin antibody, Sigma); after incubation ...
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bioRxiv - Biochemistry 2022Quote: ... The YopH tyrosine phosphatase expression vector pESU009 was generated by cloning the YopH phosphatase domain (residues 177-468) into pACYCDuet-1 (Novagen) under transcriptional control of an attenuated tac promoter.37 All constructs were confirmed by DNA sequencing.
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bioRxiv - Molecular Biology 2024Quote: cDNAs of BTB domains were PCR-amplified using corresponding primers (Supplementary Table S1) and cloned into modified pET32a(+) vector (Novagen) encoding TEV protease cleavage site after 6xHis-tag and Thioredoxin ...
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections containing SN and VTA were incubated with antibodies directed against tyrosine hydroxylase (mouse monoclonal, 1:1000, Merck-Millipore MAB318) and mCherry (rabbit polyclonal ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was blocked in 5% Milk in tris-buffered saline with 0.1% Tween 20 (TBST) and then incubated in 5% Milk TBST containing mouse anti-DPYD (Sigma, WH0001806M1) and rabbit anti-β-actin (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... The sample was then washed twice with 2X SSC and digested by 3 incubations (10 minutes for mouse and 5 min for human) with digestion buffer containing 1% (w/v) sodium-dodecyl-sulfate (SDS, Sigma), 20 mM Tris HCl (Thermo) ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated overnight at room temperature in blocking buffer containing primary antibody (mouse anti-UBE3A (Sigma SAB1404508 clone 3E5)) at 1:250) ...
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bioRxiv - Cell Biology 2021Quote: ... The flowthrough containing the mouse MLKL(1-158) construct was concentrated via centrifugal ultrafiltration (10 kDa molecular weight cut-off; Millipore) and loaded onto a Superdex 75 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse NAFs were transduced using lentiviruses containing shRNA constructs against Postn with 10 mg/ml polybrene (Sigma, TR-1003-G). shPostn1 (F primer ...
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bioRxiv - Biochemistry 2023Quote: All Gsx2 constructs were subcloned from a cDNA containing the mouse Gsx2 gene into a modified version of the pET-14b vector (Novagen) that includes an N-terminal His-tag followed by a PreScission Protease cleavage site in place of the original thrombin cleavage site ...
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bioRxiv - Cell Biology 2023Quote: PLA assays were carried out using a Duolink in situ red starter kit mouse/rabbit kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: The mNGF level in tears upon a AAV2/9 CAG-mNGF injection in the LG was dosed using the mNGF ELISA kit (Sigma-Aldrich, reference RAB1119, France) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... ELISA reaction procedure was followed as described by manufacturer (OPD; Sigma). The optical density of the supernatant was read on a spectrophotometer at 492 nm ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex ELISA was performed on a Luminex FlexMAP 3D (Millipore Sigma) using a premixed 32-plex cytokine magnetic bead panel (Millipore Sigma ...
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bioRxiv - Physiology 2021Quote: ... 100 μl of 3,3’,5,5’-tetramethylbenzidine (TMB) Elisa substrate (T0440; Sigma) was added and the reaction stopped after 10 min using 2 M H2SO4 ...
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bioRxiv - Neuroscience 2023Quote: ... Leptin concentrations were determined by ELISA (Millipore, St. Charles, MI, USA). The blood glucose levels were measured using a Glucocard glucometer (Arkray ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Cytokines and CCL5 were measured by Multiplex ELISA (MCYTOMAG-70K, Millipore) or Enzyme Linked Immunosorbent Assay (ELISA ...
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bioRxiv - Immunology 2023Quote: ... All ELISA assays were developed using TMB liquid substrate system (Sigma), stopped with 2N sulfuric acid ...
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bioRxiv - Microbiology 2023Quote: Nunc MaxiSorp flat bottom 96-well ELISA plates (Sigma-Aldrich, M9410) were coated with 50 ng/well of recombinant CCHFV Gn protein (Creative Diagnostics ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Physiology 2019Quote: ... Proteins were resuspended in concentrated urea buffer (8 M Urea, 2 M Thiourea, 150 mM NaCl (Sigma)) ...