Labshake search
Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were visualized using antibodies that recognize PSTAIRE (P7962, Sigma), V5 (Invitrogen) ...
-
bioRxiv - Genetics 2024Quote: ... Purified antibodies were incubated with magnetic G protein beads (Millipore) and separated from Fab fragments following enzymatic digestion (IdeZ ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were detected using a primary α-Flag antibody (Sigma) at a 1:3000 dilution and a secondary goat α-rabbit-HRP antibody (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
-
bioRxiv - Neuroscience 2021Quote: For phosphorylated proteins RIPA lysis buffer was further supplemented with PIC and 1X phosphatase inhibitor cocktail (Merck Millipore, cat no. 524629-1ML). A cocktail of phosphatase inhibitors (B-Glycerolphosphat (25mM) ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
-
bioRxiv - Plant Biology 2021Quote: ... the cDNA fragments coding for the mature NAD-MEβ subunits were cloned in the pET29 vector (Novagen), which adds a C-terminal His•Tag sequence ...
-
bioRxiv - Developmental Biology 2021Quote: ... Formed colonies were fixed and stained for alkaline phosphatase using Leukocyte Alkaline Phosphatase Kit (Sigma; 86R-1KT) according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... immunoprecipitants on beads were washed in buffer without phosphatase inhibitor and incubated with l phosphatase (Sigma Aldrich) in the applied buffer for 20 minutes at 30°C before elution ...
-
bioRxiv - Biochemistry 2021Quote: ... phosphatase inhibitor cocktail 2 and phosphatase inhibitor cocktail 3 were purchased from Sigma-Aldrich (St. Louis, MO). All other chemicals including V8 protease were purchased from FUJIFILM Wako ...
-
bioRxiv - Microbiology 2019Quote: Quantified protein (between 80-160 μg) was incubated with protein-specific antibodies (either R anti-HA (Sigma) or anti-NS4A ...
-
bioRxiv - Molecular Biology 2020Quote: PTP-tagged protein expression was demonstrated by immunoblotting with a rabbit polyclonal anti-protein A antibody (SIGMA) as described previously [13] ...
-
bioRxiv - Immunology 2020Quote: ... and ACE2-Fc were serially diluted in diluent buffer (1X PBS, 0.5% bovine serum albumin, 2% filtered fetal bovine serum, 0.2% bovine gamma globulins (Sigma), 5 mM EDTA ...
-
bioRxiv - Cell Biology 2020Quote: ... in lysis buffer supplemented with phosphatase and protease inhibitors (20 mM Tris-HCl pH 7.5; 150 mM NaCl; 1 % IGEPAL [vol/vol]; Phosphatase inhibitor cocktail 1:100 [Sigma-Aldrich] ...
-
bioRxiv - Biochemistry 2019Quote: ... 1 mM EGTA and 1% Triton X-100 with protease and phosphatase inhibitor cocktails both from (Millipore, Billierica, MA, USA). The soluble fractions of cell lysates were isolated by centrifugation at 15000g for 15 minutes at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... which was followed by the addition of NH4HCO3 (1 M, 3 μl, freshly made) and Calf Intestinal Alkaline Phosphatase (1 U, Sigma) and additional incubation at 37 °C for 2 h ...
-
bioRxiv - Cell Biology 2021Quote: ... 500 mM NaCl, 1 mM EDTA, 1% NP-40, supplemented with protease and phosphatase inhibitor cocktails, Roche, and Benzonase, Millipore), sonicated 3 times at low amplitude ...
-
bioRxiv - Biochemistry 2021Quote: ... 1x phosphatase inhibitors (final concentration: 10 mM NaF, 1 mM β-glycerophosphate, 1 mM Na3VO4) and 0.05 unit/μl Benzonase (Sigma). Samples were sonicated in a BioRuptorPico (Diagenode ...
-
bioRxiv - Neuroscience 2021Quote: ... with freshly added phosphatase inhibitors (50 mM NaF and 1 mM Na3VO4) and 1% (v/v) mammalian protease inhibitor cocktail (Sigma). Sonication was conducted at 50% output (Q55 Sonicator ...
-
bioRxiv - Microbiology 2019Quote: The caudal quarter of the flash frozen brain tissue was homogenized in radioimmunoprecipitation assay buffer (1% Triton-X, 0.1% SDS, 1X PBS) and 1% phosphatase inhibitor cocktail (P5726-1ML, Sigma-Aldrich) and 1% protease inhibitor cocktail (P8340-1ML ...
-
bioRxiv - Plant Biology 2019Quote: ... four times and then placed for 1 h in a solution of goat antirabbit IgG (dilution 1:17500) conjugated to alkaline phosphatase (Sigma). The membrane was rinsed in TBS-T four times ...
-
bioRxiv - Immunology 2023Quote: ... cells were lysed with chilled RIPA buffer (1% Triton X-100, 140mM NaCl, 50mM Hepes, 10mM Idoacetamide, 1×MS-SAFE Protease and Phosphatase Inhibitor (Sigma), PH7.4) ...
-
bioRxiv - Immunology 2023Quote: ... samples were lysed in a 1% SDS buffer (1% SDS, 50mM triethanolamine pH 7.4, 150mM NaCl) containing a cocktail of phosphatase protease inhibitors (Sigma, 4906845001) and protease inhibitors (Thermo Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... OC antibody of 1:25,000 and A11 antibody of 1:500 (Millipore and Life Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 µM each of leupeptin and E64 protease inhibitor and phosphatase inhibitor cocktail (Sigma, catalog# P5726). For FOXO-1 analysis ...
-
bioRxiv - Bioengineering 2021Quote: 1 × 106 cells were lysed in complete RIPA buffer with protease and phosphatase inhibitors (Sigma-Aldrich). Total protein was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) and 1x PhosSTOP phosphatase inhibitor cocktail (Sigma-Aldrich), and centrifuged at 10,000 x g for 10 minutes at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... the plate was incubated with anti-mouse IgG conjugated to alkaline phosphatase (1/4,000; Sigma-Aldrich) for 1 h at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... Posterior midguts were dissected into ice-cold PBS with phosphatase inhibitor (1:100, Sigma, cat#P5726). Guts were in ice cold PBS/4% PFA with phosphatase inhibitor and then taken through a methanol (MeOH ...
-
bioRxiv - Physiology 2021Quote: ... 1% Triton X-100 and 10% glycerol with freshly added protease and phosphatase inhibitor cocktail (Sigma). The protein concentration was determined by Bradford protein assay (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by the addition of 1M NH4HCO3 (2 mL) and 1 U of alkaline phosphatase (Sigma). The solution was incubated at 37°C for 2 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... pH 8.0) containing 1 mM PMSF and protease and phosphatase inhibitor cocktails (Sigma, # 4906845001 and #11836170001) with three 1.4 mm Zirconium oxide beads (Bertin Instruments ...
-
bioRxiv - Cell Biology 2024Quote: ... 1% (v/v) NP-40) supplemented with protease cocktail inhibitor and PhoSTOPTM phosphatase inhibitor (Sigma-Aldrich). The cell lysate was centrifuged at 16600 x g for 20 minutes at 4°C and the supernatant was collected for western blotting ...
-
bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
-
bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was used to detect protein expression using the following antibodies on paraffin sections: SftpC (rabbit, Millipore, AB37086, 1:250), and Ki67 (eBioscience ...
-
bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
-
bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
-
bioRxiv - Cell Biology 2019Quote: ... antibodies were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 (GE Healthcare Life Sciences).
-
bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
-
bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
-
bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
-
bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
-
bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...