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Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Staining was conducted in PBS / 1 % BSA (CHO cells and huPBMCs) or PBS / 1 % BSA / 1 % normal mouse serum (bovine cells) (Sigma-Aldrich) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were lysed using lysis buffer and immunoprecipitation with anti-FLAG M2 mouse (Sigma, F3165) as it was described above ...
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bioRxiv - Developmental Biology 2020Quote: ... cell lines were characterized by performing antibody staining for Flag (mouse anti-FLAG; Sigma, F1804) and HA (rabbit anti-HA ...
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bioRxiv - Physiology 2021Quote: ... mouse mucosal cells were lysed in lysis buffer (1% Triton X-100 (Sigma-Aldrich, X100), 150 mM NaCl (J.T ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were first blocked with mouse IgG 1 (Cat# l5381, Sigma, St. Louis, MO, USA) for 10 min on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysates were incubated/rotated with mouse anti-GFP antibody (Sigma-Aldrich, 1:20) at 4 °C overnight ...
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bioRxiv - Biophysics 2022Quote: ... The cells were then incubated with the monoclonal mouse anti-α-tubulin antiserum (Sigma Aldrich) diluted in a blocking buffer (1:800 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated with anti–α-tubulin primary antibody (1/1,000, Mouse, Sigma-Aldrich) for 30min ...
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bioRxiv - Biochemistry 2021Quote: Cells were probed with mouse anti-phospho-Histone H2A.X (Ser139) antibody (1:1000; Sigma-Aldrich) and rabbit anti-β-Actin (1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... Infected or transfected cells were probed with mouse anti-Myc (1:1000, Millipore 05-724), rat anti-HA (1:1000 ...
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bioRxiv - Genetics 2020Quote: Total RNA was extracted from cells or mouse tissues using TRI Reagent (Sigma-Aldrich, T9424). The quality and concentration of RNA were measured with NanoDrop ONEc (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: Whole cell lysate was incubated overnight at 4°C with mouse IgG agarose (Sigma A0919), agarose removed by centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... fixed cells were incubated with the anti-HA antibody (mouse monoclonal, clone 12CA5, Sigma, USA) for 1 hour at room temperature followed by goat-anti-mouse-Alexa Fluor 647-Plus-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with a mouse anti-GluA1 N-terminal antibody (1:100, Millipore) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was mixed with 1.2 μl of mouse anti-NeuN antibody (Millipore, MAB377) and 1 μl of anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... cells were stained as described before using 0.2 μg anti-mouse FITC antibody (Sigma-Aldrich). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... and mouse P2X4 constructs were transduced into Myc-CaP cells using polybrene (Millipore, Burlington, MA) followed by puromycin selection ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-proliferating cell nuclear antigen (PCNA) (RRID: AB_477413; Sigma, St. Louis, MO, USA, #P8825), rabbit anti-NeuN (RRID:AB_ 10807945 ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from the mouse VM after Collagenase D (Millipore Sigma, St. Louis, MO) treatment (15mg/ml ...
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bioRxiv - Biophysics 2023Quote: ... fixed HeLa cells were incubated with monoclonal mouse anti-α-tubulin (1:1000, Sigma-Aldrich) and rabbit anti-Nup153 (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then labeled using primary mouse anti-vinculin antibody (1:100; Sigma-Aldrich) and donkey anti-mouse Alexa Fluor 555-conjugated secondary antibody (1:400 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were stained with mouse α-IE-1 (Ms. X pp72 mAb, Sigma 8B1.2) and rabbit α-pp65 (Biorbyt orb10511 ...
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bioRxiv - Cell Biology 2024Quote: ... The mouse brain endothelial cell line bEnd.3 was cultured in DMEM medium (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
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bioRxiv - Biochemistry 2019Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were clarified by centrifugation before GFP-FLAG-tagged proteins were isolated by incubation with 100 µL packed FLAG M2 affinity resin (Sigma) for 1 h with rotation at 4°C ...
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bioRxiv - Microbiology 2021Quote: Samples of episomal PPS-GFP Dd2 or endogenously HA-FLAG-tagged PPS Dd2 parasites were harvested by centrifugation and treated with 0.05% saponin (Sigma 84510) in PBS for 5 min at room temperature and spun down by centrifuge at 5,000 rpm for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The resin was loaded into a column and the flow through containing S-tagged gyrase A was further purified using an S-protein agarose (Sigma) by elution with 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were hybridized overnight at 37°C with sixteen FLAP-Y-Cy-3 tagged complementary oligonucleotide probes, targeting regions in Cox7c mRNA (IDT) in Hybridization mix (15 % Formamide, 1.7 % tRNA (Sigma; R1753), 2 % FLAP:Probe mix ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Precleared lysates containing the differently tagged or untagged proteins were mixed in a 1/1 ratio and incubated with anti-FLAG-agarose beads (Sigma) overnight at 4°C to precipitate the FLAG-tagged proteins ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Transduced cell populations were selected with puromycin (2 μg/ml) and expression of flag-tagged NPC1 was confirmed by immunostaining with an anti-Flag M2 antibody (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... U2OS cells plated in six well plates were transfected with 1.8□μg of C-terminally tagged histone H3-HaloTag Fusion Vector DNA and 0.2□μg of C-terminally tagged NSD2-PWWP1-NanoLuc (Wt or F266A) or NSD3-PWW1-NanoLuc using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Biochemistry 2022Quote: ... The purification of 3XFLAG-tagged 26S proteasome was carried out by affinity purification using M2 anti-FLAG beads (Sigma A2220). After incubation for 1.5 hrs at 4°C the proteasome was eluted with FLAG peptide ...
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bioRxiv - Genetics 2022Quote: ... Degradation of the AID-tagged RAD21 was induced with addition of 500 μM indole-3-acetic acid (auxin; Sigma-Aldrich). Degradation of AID-tagged WAPL was induced with addition of 1μM 5-Ph-IAA (modified auxin ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged protein detection was performed as previously reported [71] using an Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) and bands were visualized using an ImageQuant LAS 4000 imager.
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged [P2G]CXCL12 was detected using a rabbit anti-HA primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #SAB4300603) and IRDye® 800CW-conjugated goat anti-rabbit IgG (1:20,000 dilution ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).