Labshake search
Citations for Millipore Sigma :
1351 - 1400 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
-
bioRxiv - Microbiology 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated using a 30K MWCO concentrator (EMD Millipore) to 0.2 ml and diluted to 0.5 ml in GF150 buffer (25 mM HEPES [pH7.4] ...
-
bioRxiv - Biochemistry 2024Quote: ... which contained soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
-
bioRxiv - Biochemistry 2024Quote: ... which containing soluble FLAG-tagged HsTRPM4 mixed with 1ml of Anti-FLAG® M2 affinity gel (Millipore, Billerica, MA) pre-equilibrated with wash buffer containing 25 mM HEPES-NaOH ...
-
bioRxiv - Cell Biology 2024Quote: ... 3FLAG-6HIS-YFP-tagged proteins expressed from the endogenous locus were immunoprecipitated using anti-FLAG M2 antibodies (F3165, Sigma) (Unnikrishnan et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
-
bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
-
bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
-
bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
-
bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
-
bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
-
bioRxiv - Cell Biology 2019Quote: ... The His-tag was removed by cleavage with 16 U of thrombin (Ref 27-0846-01, Sigma) added directly onto the beads for 2h at 25°C ...
-
bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
-
bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
-
bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
-
bioRxiv - Biochemistry 2023Quote: ... After several rounds of washing with His-AC washing buffer (supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
-
bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... Anti-mouse agarose or mouse agarose (both Sigma) were added for 1 hour at 40C prior to 3 washes in Lysis Buffer ...
-
bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
-
bioRxiv - Immunology 2023Quote: ... Staining was conducted in PBS / 1 % BSA (CHO cells and huPBMCs) or PBS / 1 % BSA / 1 % normal mouse serum (bovine cells) (Sigma-Aldrich) for 40 min at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were lysed using lysis buffer and immunoprecipitation with anti-FLAG M2 mouse (Sigma, F3165) as it was described above ...
-
bioRxiv - Developmental Biology 2020Quote: ... cell lines were characterized by performing antibody staining for Flag (mouse anti-FLAG; Sigma, F1804) and HA (rabbit anti-HA ...
-
bioRxiv - Physiology 2021Quote: ... mouse mucosal cells were lysed in lysis buffer (1% Triton X-100 (Sigma-Aldrich, X100), 150 mM NaCl (J.T ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were first blocked with mouse IgG 1 (Cat# l5381, Sigma, St. Louis, MO, USA) for 10 min on ice ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cell lysates were incubated/rotated with mouse anti-GFP antibody (Sigma-Aldrich, 1:20) at 4 °C overnight ...
-
bioRxiv - Biophysics 2022Quote: ... The cells were then incubated with the monoclonal mouse anti-α-tubulin antiserum (Sigma Aldrich) diluted in a blocking buffer (1:800 ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated with anti–α-tubulin primary antibody (1/1,000, Mouse, Sigma-Aldrich) for 30min ...
-
bioRxiv - Biochemistry 2021Quote: Cells were probed with mouse anti-phospho-Histone H2A.X (Ser139) antibody (1:1000; Sigma-Aldrich) and rabbit anti-β-Actin (1:2000 ...
-
bioRxiv - Microbiology 2020Quote: ... Infected or transfected cells were probed with mouse anti-Myc (1:1000, Millipore 05-724), rat anti-HA (1:1000 ...
-
bioRxiv - Genetics 2020Quote: Total RNA was extracted from cells or mouse tissues using TRI Reagent (Sigma-Aldrich, T9424). The quality and concentration of RNA were measured with NanoDrop ONEc (Thermo Scientific) ...
-
bioRxiv - Microbiology 2019Quote: Whole cell lysate was incubated overnight at 4°C with mouse IgG agarose (Sigma A0919), agarose removed by centrifugation ...
-
bioRxiv - Microbiology 2019Quote: ... fixed cells were incubated with the anti-HA antibody (mouse monoclonal, clone 12CA5, Sigma, USA) for 1 hour at room temperature followed by goat-anti-mouse-Alexa Fluor 647-Plus-conjugated secondary antibody (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with a mouse anti-GluA1 N-terminal antibody (1:100, Millipore) overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... the cell suspension was mixed with 1.2 μl of mouse anti-NeuN antibody (Millipore, MAB377) and 1 μl of anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were stained as described before using 0.2 μg anti-mouse FITC antibody (Sigma-Aldrich). Finally ...
-
bioRxiv - Cancer Biology 2021Quote: ... and mouse P2X4 constructs were transduced into Myc-CaP cells using polybrene (Millipore, Burlington, MA) followed by puromycin selection ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse anti-proliferating cell nuclear antigen (PCNA) (RRID: AB_477413; Sigma, St. Louis, MO, USA, #P8825), rabbit anti-NeuN (RRID:AB_ 10807945 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were immunostained with mouse anti-TOMM20 antibody (1:100 dilution, clone 4F3, Sigma-Aldrich), rabbit anti-LAMP1 antibody (1:100 dilution ...
-
bioRxiv - Biophysics 2023Quote: ... fixed HeLa cells were incubated with monoclonal mouse anti-α-tubulin (1:1000, Sigma-Aldrich) and rabbit anti-Nup153 (1:500 ...
-
bioRxiv - Immunology 2023Quote: Single-cell suspensions from the mouse VM after Collagenase D (Millipore Sigma, St. Louis, MO) treatment (15mg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... The mouse brain endothelial cell line bEnd.3 was cultured in DMEM medium (Sigma-Aldrich) supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were stained with mouse α-IE-1 (Ms. X pp72 mAb, Sigma 8B1.2) and rabbit α-pp65 (Biorbyt orb10511 ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were then labeled using primary mouse anti-vinculin antibody (1:100; Sigma-Aldrich) and donkey anti-mouse Alexa Fluor 555-conjugated secondary antibody (1:400 ...
-
bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
-
bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
-
bioRxiv - Biochemistry 2019Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
-
bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...