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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Eluted recombinant proteins were concentrated in Amicon Ultra-4 centrifugal filter units with 30-kDa cutoff (Millipore), concentrations were determined using the DC BCA protein assay (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Biochemistry 2020Quote: Mouse anti-FLAG M1 antibody and mouse anti-Sulfotyrosine antibody (Sigma) were used to detect the purified wild type C5aR and C5aRΔTyr with Y11F and F14F mutations respectively in the western blotting assays.
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal mouse anti-mouse GFAP (1:2000; Sigma G-A-5), and monoclonal rat anti-mouse IL-6 (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-β-actin (Sigma, mouse monoclonal antibody, 1:5000). After incubation with HRP-conjugated secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Mouse Monoclonal anti-mouse Glutamin Synthetase (GS-6, 1:250, Millipore), Rabbit Polyclonal anti-SDS (PA5-58704) ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-NeuN (mouse monoclonal, 1:400, Millipore; catalog #MAB377, RRID:AB_2298772). This step was followed by incubation with secondary antibodies for 2h at RT to visualize primary antibodies ...
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bioRxiv - Genomics 2020Quote: ... and ESC lines stably expressing 3xHA-tagged open reading frames were cultured in the presence of 1 μg/mL puromycin (Sigma-Aldrich, Cat# P8833).
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bioRxiv - Neuroscience 2019Quote: Mouse cortical or hippocampal neurons expressing extracellular HA-tagged Nrxn1α for 30 hr were either treated with 20 μM Dynasore (Sigma-Aldrich, Cat #D7693) or DMSO ...
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bioRxiv - Biochemistry 2020Quote: ... RPS2-Flag tagged 40S ribosomes were immuno-precipitated by addition of 50 µL washed anti-Flag M2 affinity agarose gel (Sigma Aldrich, A2220-5mL) and 5 µL RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpressed 3X FLAG-tagged cyclin-Cdk1 complexes were then purified by immunoaffinity chromatography using ANTI-FLAG M2 affinity agarose beads (Sigma-Aldrich Cat #A2220) and eluted with 0.2 mg/mL 3X FLAG peptide (Sigma-Aldrich Cat #F4799) ...
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bioRxiv - Pathology 2020Quote: ... Expression of human COMP in RCS cells was validated by Western Blot with anti-Flag antibody to recognize the tagged COMP (1:5000 F7425 rabbit polyclonal from Sigma, Saint Louis, MO). DOX was used for 4 days to induce COMP expression in RCS cells ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated with primary antibody Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit for detection of TAP-tagged proteins (Sigma-Aldrich, P1291-500UL) 1:1000 dilution in 5 % skimmed milk/PBST overnight at 4 °C followed by washing in 0.1%Tween/TBS ...
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bioRxiv - Biochemistry 2024Quote: ... adhered cells were washed with PBS and treated with cMyc-tagged CK2α or ZHER2-CK2α (1 μM) in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). Cells were incubated at 37 °C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were reprobed with a mouse monoclonal antibody anti-β-actin (clone C4, Millipore; 1:5,000 dilution).
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bioRxiv - Microbiology 2019Quote: ... Cells were then blocked with ultra-block solution (10% rat sera, 10% hamster sera, 10% mouse sera (Sigma), and 10 μg/ml of anti-CD16/32 (2.4G2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Ladi2.1 and Ladi3.1 cells (derived from p53fl/fl-LSL KRASG12D/+ mouse NSCLC model) were cultured in DMEM (Sigma). Human cells were STR-profiled ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNAs stably integrated in mouse and human cells were selected with 10 and 2 μg/ml puromycin (Sigma), respectively ...
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bioRxiv - Microbiology 2020Quote: ... The fixed cells were incubated with both primary antibodies anti-T7 epitope mouse monoclonal antibody (Novagen, 69522-4) and anti-HA goat polyclonal antibody (GenScript ...
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bioRxiv - Microbiology 2020Quote: ... the cells were permeabilized in 0.5% saponin in PBS and stained with mouse anti-Flag M2 antibody (Sigma) and homemade rabbit anti-Cap1 antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at 4 °C with anti-phospho-histone H2A.X (Ser139) monoclonal mouse antibody (Millipore, clone JBW301) at 1:1000 dilution ...
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bioRxiv - Biophysics 2022Quote: ... cells were incubated for 48 h at 4 °C with a mouse anti-α-tubulin (DM1A; Sigma-Aldrich) primary antibody at 1:5,000 in PBT ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then centrifuged and resuspended in diluted Alexa Fluor 647-conjugated mouse anti-γ-H2AX antibody (Millipore, 05-636-AF647 ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were subsequently stained with mouse or rabbit anti-HA antibody (Sigma-Aldrich H3663 or H6908, 1:1000) and the following Golgi markers ...
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bioRxiv - Genetics 2020Quote: ... Spo11-oligo complexes were immunoprecipitated from whole-cell extracts by using mouse monoclonal anti-Flag M2 antibody (Sigma). Precipitated Spo11-oligo complexes were end-labeled with [α-32P] dCTP in a terminal deoxynucleotidyl transferase reaction ...
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bioRxiv - Biochemistry 2021Quote: ... V5-ABCF1 knock-in D3 mouse ES cells were treated with DMSO or etoposide (ETO, 20 μM; Sigma) for 6 hr ...
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bioRxiv - Cell Biology 2021Quote: ... mixed glial cell culture from each mouse was isolated by homogenizing the brain in a glass homogenizer (Sigma) and then purified by mixing 17 mL PBS brain lysate with 4,5 mL Percoll Plus (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Cleavage efficiency of the selected sgRNAs was tested using cel-1 assay in mouse neuroblastema (N2a) cells (SIGMA) with the primer pairs listed in Table S1 ...
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bioRxiv - Cell Biology 2021Quote: All mouse stem cells were maintained on gelatin-coated plates in the ESGRO Complete Clonal Grade Medium (Millipore), and then cultured in the M10 medium (High glucose DMEM ...
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bioRxiv - Immunology 2020Quote: ... cells were then incubated with an anti-flag monoclonal (Anti-Flag M2 produced in mouse, Sigma cat# F1804) antibody at 1:1000 in phosphate buffered saline with 3% BSA overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with rabbit anti-protein VI 26 or mouse anti-E1A clone M73 (Millipore, 05-599) diluted in blocking buffer (10% goat serum in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The presence of ZIKV in infected cells was detected with a mouse anti-flavivirus 4G2 primary antibody (Millipore) and a donkey anti-mouse IgG horseradish peroxidase-conjugated secondary antibody (Jackson Research Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C with primary antibody mouse anti-Flag antibody (1:1000, Sigma). After three washes in PBS (5 min each) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit anti-GFP (2.5 µg/ml) and mouse DM1α (1:500, Sigma-Aldrich T6199), rabbit anti-Kif18B serum (1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then centrifuged and resuspended in diluted Alexa Fluor 647-conjugated mouse anti-γ-H2AX antibody (Millipore, 05-636-AF647 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then immunolabeled with primary antibodies specific for FLAG (mouse anti-FLAG M2, Sigma, F3165, 1:200), T7 (rabbit anti-T7 ...
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bioRxiv - Systems Biology 2023Quote: Cells were prepared for flow cytometry by first performing a blocking step with 5μl mouse serum (Sigma-Aldrich) then cells were resuspended in FACS Buffer containing 0.5% Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and cells were probed using primary antibodies to VZV proteins IE62 (mouse mAb; EMD Millipore MAB8616; 1:200), capsid-ORF23 (polyclonal rabbit ...
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bioRxiv - Neuroscience 2023Quote: Mouse High Sensitivity T Cell Magnetic Bead kits were purchased from EMD Millipore (MHSTCMAG-70KPMX, Burlington, MA, USA) and used to quantify 18 different mouse cytokines in accordance with the manufacturer’s recommendations (belonging to 4 main categories ...