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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... Degradation of AID-tagged CHD4 was induced by the addition of 500 μM 3-Indoleacetic acid (I2886, Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... The GST-tagged proteins were eluted by 10mM reduced glutathione (Sigma, 100mM stock made in 50mM Tris, pH7.6) in PBS (containing 1mM PMSF and 0.02% (∼3mM ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Cell Biology 2023Quote: 75 μg GST-tagged Rab GTPase was loaded with either 10 mM GDP or GTP (Sigma Aldrich, Germany) in the presence of 20 mM EDTA and 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to binding of FLAG-tagged peptide species with anti-FLAG M2 magnetic beads (Sigma, cat # M8823). The purified FLAG-tagged peptides were harvested by eluting from the anti-FLAG M2 magnetic beads with 100 ng/µl 3xFLAG peptides.
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged protein was eluted off the beads by using 20 mM glutathione solution (Sigma-Aldrich, #G4251-5G). Fractions were analyzed by resolving on 4-20 % gradient SDS-PAGE (BioRAD #4561094 or #4651093) ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins was performed with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat.no. A2220-1ML) at 4 °C with overhead rotation for 6 h ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Bioengineering 2022Quote: ... was started by temporal modulation of Wnt signaling using small molecules (CHIR99021, Tocris, R&D Systems, Cat. No. 4423, and IWR-1, Sigma-Aldrich, Cat. No. I0161). The E8 medium was switched out for B27(-)/CHIR ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were probed with mouse anti-anillin monoclonal primary antibody (1:100, Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... cells were first incubated with mouse monoclonal anti-cadherin antibody (Sigma-Aldrich, MO, USA) diluted ×200 in PBS with a supplement of 10% BSA for 45 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with 1:1000 mouse monoclonal anti-FLAG M2 antibody (Sigma, F1804) in blocking buffer for 1 hour at RT ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with 5% BSA and incubated with a FLAG antibody (Sigma, mouse) at 1:500 dilution in 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Fixed cells were incubated with anti-T7e epitope mouse monoclonal antibodies (Novagen, 69522-4) and anti-HA rabbit polyclonal antibodies (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with a mouse anti-DENV complex monoclonal antibody (MAB8705, Merck Millipore), washed three times with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The mouse ovarian surface epithelial cell line ID8 was purchased from Sigma-Aldrich (SCC145). Cell lines were validated by STR profiling ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were subsequently stained with mouse anti-HA antibody 1:1000 (Sigma-Aldrich H3663) and rabbit pRab10 1:1000 (Abcam ab230261) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were subsequently stained with mouse anti-HA antibody 1:1000 (Sigma-Aldrich H3663) and rabbit pRab10 1:1000 (Abcam ab230261) ...
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bioRxiv - Immunology 2019Quote: ... Cells were sequentially stained with the following antibodies: mouse anti-neutrophil elastase (MABS461, Millipore) at 1:250 ...
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bioRxiv - Immunology 2019Quote: ... The cells were then incubated with the monoclonal mouse anti-Flag antibody (F3165, Sigma) and Monoclonal rabbit anti-HA (H6908 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained a monoclonal primary mouse anti-FLAG M2 antibody (Millipore Sigma F3165) at a dilution of 1:250 and incubated in a light-blocking incubation chamber overnight with 4°C ...
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bioRxiv - Immunology 2020Quote: ... The cells were then incubated with the monoclonal mouse anti-Flag antibody (F3165, Sigma) and Monoclonal rabbit anti-HA (H6908 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 1:200 mouse anti-FLAG M2 antibody (Sigma-Aldrich, F3165) for 2 hours at RT ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were then incubated with monoclonal mouse anti-α-tubulin antiserum (Sigma-Aldrich) diluted in blocking buffer (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with a different GluA1 antibody (Millipore – MAB2263, mouse, 1:500) to allow for staining of the remaining surface GluA1 (not endocytosed receptor) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were incubated with either mouse anti-β3-tubulin (TUJ1; 1:1000; Sigma) or mouse anti neuronal nuclei (NeuN ...
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bioRxiv - Cell Biology 2020Quote: ... Immunofluorescence in human cells was conducted using antibodies against mouse □-tubulin (Sigma, 1:1000), mouse CENP-E (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with mouse monoclonal antibody against α-tubulin (1:200; Sigma-Aldrich), followed by incubation with donkey Dylight-conjugated polyclonal antibody against mouse IgG (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 20 nmol siRNA (mouse Lap2: 5’-GAUGUGACAGAGCUCUCUA predesigned from Sigma; mouse Lap2α ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with primary antibody mouse anti-FLAG (1:1,000, Sigma #F1804) overnight at 4 °C with gentle rocking to detect FLAG-tagged viral RNRs ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were stained with antibodies against AnkG (mouse monoclonal, 1:1000; Millipore, MABN466), pCaMKII (rabbit polyclonal ...
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bioRxiv - Neuroscience 2022Quote: The mouse microglial BV2 cell line was maintained in complete RPMI-1640 medium (Sigma), 1% GlutaMAX ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated overnight with antibodies targeting tubulin (Sigma T6199, DM1α, 1:1000, mouse), EB1 (Millipore AB 6057 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained with the following primary antibodies: mouse anti-γ-tubulin (Sigma, Germany), rabbit anti-SIK2 (Cell signaling) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Cell SignalingTechnology) or with a mouse monoclonal anti-acetylated tubulin antibody (T6793, Sigma-Aldrich) at 1:250 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-4C4 primary was isolated from 7.4.C4 mouse hybridoma cells (Sigma, 92092321-1VL) as per manufacturer instructions.
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bioRxiv - Developmental Biology 2022Quote: ... Cy3-mouse-anti-mouse aSMA (Sigma C6198), mouse anti-mouse αSMA (Sigma A2547) ...