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Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the following primary antibody: mouse-monoclonal anti-γ-H2AX (Millipore, 1:1000). Immunofluorescence for G-quadruplex structures (G4s ...
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bioRxiv - Cell Biology 2023Quote: ... bone marrow cells harvested from mouse femurs and tibias were centrifuged with Histopaque 1119 (Millipore Sigma) and Histopaque 1077 (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary mouse neurons were seeded (8 x104 cells/well) in a poly-L-lysine (PLL, Sigma) coated 96-well plate (flat bottom ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained by goat anti-mouse β-tubulin (catalog no. T4026, Sigma, 2.4 mg/ml) with 1:1,000 dilution in 3% BSA for 1 h and washed 3 times for 5 minutes each with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The cells on cover slips were then incubated with Cy3-conjugated anti-mouse secondary antibodies (Millipore) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse embryonic stem cell medium was prepared by supplementing EmbryoMax® DMEM (Millipore, SLM-220-B) with 15% FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitation (IP) of Flag-tagged ULK1 construct was performed using EZviewTM Red ANTI-FLAG ® M2 Affinity Gel (Sigma-Aldrich, F2426) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... GST and GST-Rab11DN were purified from the supernatant with Glutathione Sepharose 4B GST-tagged protein purification resin (GE17-0756-01, Sigma-Aldrich) using batch method ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein lysates were diluted to 1 ml with CoIP Lysis Buffer and the 3xFLAG-tagged bait was immunoprecipitated by incubation with 20 μl of anti-FLAG M2 Magnetic Beads (Sigma M8823) for 2 hrs at 4 °C on a tube rotator ...
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bioRxiv - Plant Biology 2020Quote: ... the FLAG-tagged proteins were immunoprecipitated by incubation of the extracts with 5 μL of anti-FLAG antibody agarose beads (Sigma-Aldrich) for 1 hr at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... 17 µL of FLAG-tagged effector protein extract was added to the beads with 1 µL of 1 mM acetyl-CoA lithium salt (Sigma-Aldrich) and 2 µL of 1 µM IP6 (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: Primer sequences for markers were obtained from the UCSC genome browser and standard HEX-tagged primers (Sigma-Aldrich, Supplementary Table 2) were used to assess the flanking haplotypes of MMP20 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged proteins were probed with immunoblotting using monoclonal anti-Flag M2-Peroxidase (HRP) antibody (Sigma-Aldrich, Saint Louis, MO, USA) (1/50,000X dilution ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal FLAG-tagged OCT4 and various SOX TFs were generated by subcloning cDNAs into pFLAG-CMV5a mammalian expression vector (Sigma-Aldrich). For stable expression of mCherry and SOX15 in OEC-2 cells ...
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bioRxiv - Microbiology 2021Quote: ... The HS and CS samples were analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) and quantified by inclusion of [13C6]aniline-tagged standard HS disaccharides (Sigma-Aldrich), as described (Lawrence et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The blot was stripped and then reprobed for FLAG-tagged receptor using anti-FLAG antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... The EGFP-FLAG-tagged proteins were eluted from the beads with 0.1 mg/mL 3x FLAG peptide (Sigma-Aldrich, Cat. #F4799) in TBS at 4°C for more than 1 hr ...
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bioRxiv - Genomics 2021Quote: ... Degradation of the AID-tagged RAD21 was induced by the addition of 500 μM (final concentration) indole-3-acetic acid (IAA/auxin; Sigma Aldrich) during media change 24 hours prior to imaging if applicable.
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bioRxiv - Cell Biology 2020Quote: Purification of mammalian Cavin1 was performed by Transfecting GFP-tagged Cavin1 using polyethylenimine (PEI) transfection reagent (Sigma-Aldrich Cat. No. 408727) with 1:4 w/w ratio (DNA:PEI ...
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bioRxiv - Microbiology 2021Quote: ... The FLAG-tagged protein-DNA complex was purified via affinity chromatography using anti-FLAG M2 affinity gel (Sigma-Aldrich, Taufkirchen, Germany). The purified protein-DNA complexes and the control were incubated at 95 °C for 40 min for reversal of the crosslink ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Molecular Biology 2022Quote: ... cell surface HS level was examined after staining with anti-HS antibody (10E4, amsbio) at 5 μg/ml and FLAG-tagged enzymes were detected by using anti-FLAG® M2 antibody (Sigma), at 2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Molecular Biology 2023Quote: ... V5-tagged protein was precipitated with 20 μl anti-V5 beads (Anti-V5 Agarose Affinity Gel, Cat.- No. A7345, Sigma-Aldrich) rotating on a wheel for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... resolved by SDS-PAGE and immunoblotted for the presence of the HA- tagged effector using α-HA antibody (H9658; Sigma-Aldrich) and α-HA-HRP (3F10 ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitates were washed three times with RIPA buffer and Flag-tagged proteins were eluted by two consecutive elution steps with 400 µg/ml Flag-peptide (Sigma-Aldrich) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... MBP- and GST-tagged proteins were purified by gel filtration on a Superdex 200 column (Sigma-Aldrich Cat# GE17-5175-01) and concentrated using a 30K Amicon filter.
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA-tagged antibody conjugated with a PLUS oligonucleotide was prepared using the Duolink In Situ Probemaker PLUS (DUO92009; Sigma-Aldrich). The specimens were washed twice with wash buffer A and were subsequently incubated with Duolink ligation solution for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged Af1521 macrodomain beads were produced in-house using BL21(DE3) bacteria and coupled to glutathione Sepharose 4B beads (Sigma-Aldrich), essentially as previously described ...
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bioRxiv - Molecular Biology 2021Quote: ... elution profiles and sample purity were confirmed by Western blot with antibodies against His tag (Sigma, H1029, lot 033m4785) and Strep tag (IBA ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+ -NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...