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Citations for Millipore Sigma :
1351 - 1400 of 1404 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... protein extracts from the insoluble fraction were diluted in blue native sample buffer (25 mM BisTris, Bio-Rad; 0.015 1N Hydrochloric Acid, Fisher Scientific; 10% glycerol, Sigma Aldrich; 25 mM NaCl ...
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Evidence for Involvement of WDPCP Gene in Alcohol Consumption, Lipid Metabolism, and Liver CirrhosisbioRxiv - Genetics 2023Quote: ... 30 µL of each sample was transferred into a clear-bottom plate (two technical replicates per biological sample) together with 100 µL of free glycerol reagent (Sigma‒Aldrich, F6428) and incubated at 37°C for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... Conidial suspensions in water to be used for inoculations were prepared by spreading conidia from glycerol stocks onto Potato Dextrose Agar (PDA, Sigma Aldrich, UK) plates ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted on glass microscope slides using mounting solution (0.1M Tris-HCl, pH 8.5, 10% Mowiol [Sigma, UK] and 50% glycerol) containing 2.5% DABCO (1,4 diazabicyclo(2.2.2)octane ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasma was stored at −30°C for analysis of leptin and insulin (CrystalChem Inc., Chicago, IL, USA) and triglyceride content (Roche triglyceride reagent, Sigma glycerol standard).
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested in ice and suspended in buffer (25 mM Tris pH 8.0, 200 mM NaCl, 10 mM imidazole, 10 % glycerol, 5 mM β-mercaptoethanol, and SIGMA protease inhibitor cocktail), treated with 25 μM PMSF immediately before lysis by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then mounted in 12 µL of mounting media (20 M N-propyl gallate [Sigma-Aldrich] and 0.14 M Tris in glycerol) with a no ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set, and Sigma phosphatase inhibitor set), incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... the affinity gel was suspended in elution buffer (2% SDS, 50 mM Tris pH 7.5, 50 mM NaCl, 20% glycerol, 2 mM dithiothreitol (Sigma-Aldrich #D0632-5G; fresh) and incubated at 65°C for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The triglyceride content of the boiled samples was determined by comparison to a 2.5 mg/mL glycerol standard (Sigma, St. Louis, MO, USA) using the Sigma serum triglyceride kit (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The BenchSmart 96-well pipetting robot was used along with a P1000 head and a 300-mL reservoir to transfer 353 µL of a sterile solution of 50% glycerol (Fisher, Cat. #G33-4) and 50 mM cysteine (Millipore, Cat. #243005-100GM) that had been pre-reduced inside the chamber for >48 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% glycerol, 1% Triton X-100, 10 µM MgCl2), supplemented with phosphatase inhibitor (Bio tool, B15001A/B) and protease inhibitor (Millipore Sigma, P2714-1BTL) cocktails ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA, 10mM sodium pyrophosphate, 10 mM glycerol 2-phosphate, 50 mM NaF, 0.5 mM sodium orthovanadate and Sigma protease inhibitor cocktail P8340). Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MgCl2, 100 mM KCl, 10% glycerol, 0.05% NP-40, cOmplete™ EDTA-free Protease Inhibitor Cocktail tablet [Sigma], NaF, Na3VO4, PMSF). Worm pellets were resuspended in fresh lysis buffer (up to 2 ml ...
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bioRxiv - Microbiology 2022Quote: ... Cells were harvested ~24 hours after transfection and resuspended in 500 µl of lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10% glycerol, 0.5% IGEPAL, 100 μg/ml RNaseA, 1 tablet of Sigma-Aldrich cOmplete Protease Inhibitor Cocktail). Resuspensions were vortexed ...
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bioRxiv - Plant Biology 2022Quote: ... 20 mM Tris–HCl [pH 8.0], 5 mM imidazole, 5% glycerol, 1 mM PMSF, 1/10 protein inhibitor cocktail: Sigma, St. Louis, MO, USA). After centrifugation for 40 min at 21,000 xg ...
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bioRxiv - Physiology 2022Quote: Tissue triglyceride levels were measured using an enzymatic triglyceride assay (Glycerol Reagent, Cat# F6428, Triglyceride Reagent, Cat#T2449, Sigma-Aldrich, Oakville, ON, Canada). In a flat bottom 96 well plate ...
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bioRxiv - Microbiology 2023Quote: ... All strains were maintained at −80°C in a 1:1 mixture of 40% glycerol (20% final concentration) and lysogeny broth (LB, Sigma Aldrich, Miller formulation) amended to 3% w/v NaCl (termed LBS 3%) ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were collected sequentially in six subseries and stored until use at -20°C in Eppendorf tubes with cryoprotective solution (30% ethylene glycol, 30% glycerol and 40% PB 0.1M; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biochemistry 2023Quote: ... protein extracts from the insoluble fraction were diluted in blue native sample buffer (25 mM BisTris, Bio-Rad; 0.015 1N Hydrochloric Acid, Fisher Scientific; 10% glycerol, Sigma Aldrich; 25 mM NaCl, Sigma Aldrich; 0.001% Ponceau S ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in selective lysis buffer (50 mM HEPES [pH 7.9], 150 mM NaCl, 10% glycerol, 1% IGEPAL) supplemented with protease inhibitors (Sigma-Aldrich catalog number P1860), incubated on ice for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the retinal flat mount samples were placed as four-radial incisions and were prepared on slides in PBS with 10% glycerol (Sigma, St. Louis, MO, USA). The retinal slides were examined under a fluorescence microscope (Olympus BX-50 ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then washed three times in 1× PBST prior to mounting on glass slides in glycerol with Mowiol 4-88 anti-fade agent (EMD Millipore Corp., Billerica, MA). When required ...
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bioRxiv - Plant Biology 2021Quote: ... 50 mM Tris–HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Triton X-100, 1×Proteinase inhibitor cocktail SIGMA P9599 and 1 mM EDTA) and incubate the extract at 4 °C for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... frozen leaf samples were ground in liquid nitrogen and incubated in 4 ml lysis buffer (50 mM Tris-HCl pH7.5, 150 mM NaCl, 5% glycerol, 0.05% NP-40, 1 mM MgCl2, 0.5 mM PMSF, 1X Sigma protease inhibitor, 10 mM DTT, 2% PVPP) at 4 °C for 15 min using a rotator to form a homogeneous suspension ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by stirring for 1 hour in lysis buffer (100 mM Tris, 150 mM NaCl, 10% glycerol, 6 mg/mL lysozyme (Sigma, St. Louis, MO, USA), 2 mg/mL deoxycholic acid ...
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bioRxiv - Genetics 2020Quote: The gonads were washed 2 times for a minimum of 10 minutes each and dehydrated in a series of glycerol (Sigma-Aldrich, Catalog #: G5516-1L) washes for 1 hour minimum each ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 100 µL of reaction buffer (25 mM HEPES, 15 mM EDTA, 10% glycerol, 1 tablet of Sigma-Aldrich cOmplete Protease Inhibitor Cocktail), and subjected to freeze-thaw ...
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bioRxiv - Cancer Biology 2022Quote: ... FLAG-IP lysis buffer (50 mM HEPES pH 8.0, 100 mM KCl, 2 mM EDTA, 0.1% NP40, 10% glycerol, 1 mM PMSF, 1 mM DTT, and Sigma protease inhibitors P8340 1:500). Resuspended lysates were first froze and thawed twice (dry ice and 37 °C waterbath ...
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bioRxiv - Cell Biology 2022Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with H33342 nuclear dye (Sigma, B2261, 1 μg/ml) and ready for imaging ...
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bioRxiv - Biochemistry 2022Quote: ... Magnetic beads containing FLAG-tagged protein were washed four times with FLAG wash buffer (20 mM HEPES, pH 7.4, 100 mM NaCl, 0.05% [w/v] digitonin [Sigma: D141], and 10% [w/v] glycerol) and eluted with FLAG elution buffer (20 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... triglyceride and glycerol (Triglyceride-SL Assay, Cat# 236-60, Sekisui Diagnostics, PEI, Canada) and pyruvate (Pyruvate Assay Kit, Cat#MAK071, Sigma-Aldrich, Oakville, ON, Canada) were measured using colorimetric assays according to the manufacturers’ directions ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μl of reaction buffer (25 mM HEPES, 15 mM EDTA, 10% glycerol, 1 tablet of Sigma-Aldrich cOmplete Protease Inhibitor Cocktail), and sonicated at the lowest setting ...
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bioRxiv - Neuroscience 2023Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with PI (Sigma, P4170, 10 μg/ml) as a red nuclear fluorescence dye and ready for imaging ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were obtained from cell pellets after lysis with 100 μL to 200 μL of sodium-dodecyl-sulfate (SDS) lysis buffer (2% SDS, 10% glycerol, 50 mM of Tris HCL) plus protease inhibitor cocktail (Sigma Aldrich, Catalog. No. 11836170001). Cell lysates were centrifuged for 15 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 100 mg per human stool sample were suspended by gentle mixing in 1 ml of reduced PBS supplemented with 0.1% L-cysteine and 15% glycerol (Sigma‒Aldrich, St. Louis, MO, USA) in an anaerobic chamber (Whitley DG250 Anaerobic Workstation) ...
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bioRxiv - Biochemistry 2023Quote: ... ammonium formate (AF), carboxymethyl cellulose (CMC), glycerol, and 2,5-dihydroxyacetophenone (DHA, further purified by recrystallization) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Isopentane ...
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bioRxiv - Biochemistry 2023Quote: ... 1,3(d5)-dipentadecanoyl-2-(9Z-octadecenoyl)-glycerol (15:0/18:1/15:0-d5 TG) were obtained from Sigma Aldrich (St Quentin-Fallavier, France). QReSS kit containing labelled metabolites (stable isotope-labelled molecules was obtained from Cambridge Isotope Laboratories Inc ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we filled each well of a 96 deepwell plate (1mL, Polypropylen; #135702; Dutscher, Bernolsheim, France) with 250µL of 60% (v/v) Glycerol (#G6279; BioXtra, ≥99% Sigma-Aldrich St. Louis, MO, USA) and eleven wells per strains (one strain per row ...
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bioRxiv - Microbiology 2024Quote: ... using 100 mg of human stool sample per 1 ml of PBS supplemented with 0.1% L-cysteine and 15% glycerol (Sigma-Aldrich, St-Louis, MO, USA). The suspension was incubated for 10 min to allow large particles to settle ...
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bioRxiv - Plant Biology 2022Quote: Authentic standards of sugars (rhamnose, arabinose, fructose, glucose, maltose, sucrose, and melibiose) and sugar alcohols (glycerol, myoinositol, arabitol, and sorbitol) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Internal standards including fructose-13C6 (for sugars ...
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bioRxiv - Molecular Biology 2022Quote: ... The bound proteins were eluted by Flag elution buffer (50 mM NaPi [pH7.5], 10% glycerol, 30 mM NaCl, 200 µg/ml 3xFlag peptide [SIGMA], 0.1 mM PMSF and PI tablet) (Uno et al. ...
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bioRxiv - Pathology 2023Quote: ... and then placed in a freeze protective solution (dimethyl sulfoxide in 125 mM PB with 20% glycerol, Merck Life Science AS/Sigma Aldrich Norway AS, Oslo, Norway) until sectioning ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were diluted to a final concentration of 2 mg/mL with Laemelli sample buffer (188 mM Tris-HCl, 218 mM SDS, 8% glycerol, and 500 μM Bromophenol Blue (B5525, Sigma-Aldrich, St. Louis, MO, USA). An equal amount of protein ...
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bioRxiv - Microbiology 2023Quote: Single-spore colonies were generated for each isolate and these were maintained in PDA for short-term use and as seven-day-old spore suspensions in glycerol stocks (2mL, 15%) for long-term preservation at −80°C in Nunc® CryoTubes® (Sigma-Aldrich, Roskilde, Denmark). The entire collection was deposited in the microbial culture collection of the Instituto de Investigaciones de Sanidad Vegetal (INISAV ...
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Evidence for Involvement of WDPCP Gene in Alcohol Consumption, Lipid Metabolism, and Liver CirrhosisbioRxiv - Genetics 2023Quote: ... were incubated together with either 35 µL of PBST (for free glycerol measurements) or 35 µL of TAG reagent (Sigma, no. T2449, for TAG measurements) at 37 °C for 60 min ...
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bioRxiv - Neuroscience 2023Quote: ... and then placed in a freeze protective solution (dimethyl sulfoxide in 125 mM PB with 20% glycerol, Merck Life Science AS/Sigma Aldrich Norway AS, Oslo, Norway) until sectioning ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections stained with NovaRED were dehydrated and mounted with Kaiser’s glycerol gelatine (phenol-free; Roth) or Eukitt® quick-hardening mounting medium (Sigma-Aldrich, St. Louis MO, USA). Conditions used for all antibodies were optimized using human tonsil tissue as the positive control ...
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bioRxiv - Biophysics 2023Quote: Measurements on oil droplets were performed by dispersing the concentrated droplet solution into a mixture of 83.8 v/v% glycerol (Sigma-Aldrich G5516, CAS 56-81-5) with water ...
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bioRxiv - Biophysics 2019Quote: ... 25 mL of TB medium (12 g Bacto-tryptone, 24 g Bacto-yeast extract, 0.4% glycerol v/v (Sigma Aldrich, cat# G7893, CAS: 56-81-5), brought to 900 mL with MilliQ water ...