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Citations for Millipore Sigma :
1201 - 1250 of 1404 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... pLKO.1-puro shRNA plasmid DNA was isolated from bacteria glycerol stocks (IFNAR1: TRCN0000301483, PD-L1: TRCN0000068001; Sigma Aldrich) using E.Z.N.A.® Plasmid Mini Kit I (Omega Bio-tek ...
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bioRxiv - Molecular Biology 2023Quote: The concentrated transporter-nanobody complex (35 mg/ml) was mixed with molten 1-Oleoyl-rac-glycerol (monoolein, Sigma-Aldrich) at a protein:lipid ratio of 2:3 (v/v ...
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bioRxiv - Immunology 2023Quote: Tumor cells were washed in PBS and pellets were lysed for 20 minutes on ice in lysis buffer (100mM Hepes pH 7.5, 300mM NaCl, 10mM EGTA, 3mM MgCl2, 2% glycerol, 2% triton X-100 (Sigma), protease inhibitor cocktail (Roche 5892791001 ...
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bioRxiv - Cell Biology 2023Quote: ... 30 µgs of caput and cauda amyloid fractions were incubated in a final volume of 40 µl containing dPBS and 1 µl (12.5 μg/ml final) DNase-free RNase in RNase buffer (10 mM Tris, pH 7.4, 5 mM CaCl2, 50% glycerol) (Sigma) or RNase buffer alone at 37°C for 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... plants were harvested at 4 dpi and stained with a 3:1 ethanol:lacto-phenol trypan blue solution [1:1:1:1 phenol: lactic acid:water: glycerol and 0.05% trypan blue (Sigma-Aldrich)] ...
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bioRxiv - Microbiology 2023Quote: ... Mycobacterial strains were cultured in Difco Middlebrook 7H9 liquid medium (Becton Dickinson, UK) supplemented with 0.2% glycerol (Sigma, UK), 0.05% Tween 80 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... gel samples were prepared by boiling for 5 min in 2x SDS sample buffer (125 mM Tris, 4% SDS, 20% glycerol, 0.04% bromophenol blue, pH 6.78) supplemented with 5% β-mercaptoethanol (Sigma). Samples were briefly centrifuged prior to loading onto 4-12% NuPAGE Bis-Tris mini-gels (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides went through a final wash for 30 min in PBS and mounted with glycerol containing (2.5% DABCO; Sigma). This method was used with the following antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... mixed with 4X Laemmli sample buffer (62.5mM Tris, 10% glycerol, 1%SDS, 0.005% bromophenol blue) containing beta-mercaptoethanol (M6250, Sigma-Aldrich), and incubated for 5 minutes at 95 deg C ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were diluted with 6 x Laemmli buffer (60% Tris- HCl pH 6.8, 10 % SDS, 30 % glycerol (Carl Roth, 3783) and 0.01 % Bromophenol blue (Sigma, B0126) containing 10 % β-mercaptoethanol (Carl Roth ...
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bioRxiv - Plant Biology 2020Quote: ... EE was gently removed and leaves were submerged in lactophenol trypan blue solution (5 ml of lactic acid, 10 ml of 50% glycerol, 1 mg of trypan blue (Sigma), and 5 ml of phenol ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were collected on ice in 0.5% NP40 lysis buffer (50 mM tris-Hcl pH=8. 100 mM NaCl, 10% glycerol, EDTA-free protease inhibitor cocktail (Sigma), 10 μM of MG-132 (Sigma ...
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bioRxiv - Genetics 2021Quote: Nuclei were lysed in a high salt buffer (20mM Tris-HCl, pH 7.6, 300mM NaCl, 10% glycerol, 0.2% (v/v) Igepal (Sigma-Aldrich, 630)) and were loaded on a Superose 6 10/30GL column (Amersham ...
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bioRxiv - Developmental Biology 2021Quote: ... regenerating and normal developing embryos were fixed in 4% PFA/PBS and dehydrated in increasing concentrations of glycerol (Sigma-Adrich). Images were acquired Leica MZFLIII fluorescence stereo microscope with a 1x objective fitted with a
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bioRxiv - Molecular Biology 2021Quote: ... Eluted complexes were fractionated by 10%–30% glycerol gradient and the fractions containing intact BAF complex were combined and concentrated using Amicon Ultra-4 centrifugal filter (Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were resuspended in 300 μl Breaking Buffer (100 mM Tris, 8.0, 20% glycerol, protease inhibitors (Millipore Sigma #04693159001)) ...
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bioRxiv - Molecular Biology 2021Quote: The concentrated elute was dialyzed with buffer BC-50 (BC buffer with 50 mM NaCl and 5% glycerol) and loaded on a 5 mL HiTrap Q Fast Flow anion exchange chromatography column (Sigma) using an ÄKTATM Pure FPLC (GE Healthcare) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cells were then resuspended in 500 µL of frozen competent cell solution consisting of 2.5% (v/v) glycerol (Sigma-Aldrich) and 5% (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were washed again and then mounted in 90% glycerol in PBS with 2.5 mg/mL p-Phenylenediamine (Sigma-Aldrich). Antibodies used were ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% glycerol and 2 mM β-mercaptoethanol) supplemented with 1 pill of cOmpleteTM EDTA-free Protease Inhibitor cocktail (Sigma-Aldrich). Cells were then sonicated and the lysate was clarified by centrifugation in a Beckman JA-25.50 rotor at 50000 x g for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Stock solutions were frozen at −80C in 30 percent glycerol and streaked onto fresh agar plates (Davis Minimal Media (Sigma) with 2000 µg/ml glucose ...
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bioRxiv - Plant Biology 2019Quote: ... in protein sample buffer (50 mM Tris HCl [pH 7.5], 10% glycerol, 2% SDS, 2 mM EDTA, 1 mM DTT and 1% protease inhibitor [Sigma-Aldrich]). Samples were separated by SDS-PAGE on 4-20% gradient polyacrylamide gels and transferred to Immobilon-P PVDF membranes (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed three times in PBS and mounted in 70% glycerol with with 5 μgml-1 concentration of 4-6-diamidino-2-phenylindole (DAPI, Sigma) and cells were mounted on cover slips ...
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bioRxiv - Neuroscience 2019Quote: ... For total lysate, proteins were directly solubilized in 4X loading buffer [(250 mM Tris, 40% glycerol, 0.008% bromophenol blue (all Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2020Quote: ... the embryos were cleared in 50% glycerol and mounted dorsal side down on a glass bottom petri dish using low gelling agarose (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... concentrated and buffer exchanged to the same buffer supplemented with 10% glycerol using a 10 kDa MWCO centrifugal filter (Amicon Ultra, Millipore). Aliquots were snap frozen in LN2 and stored at −80°C ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to early log phase in synthetic complete (SC) media + 2% glycerol + 1% ethanol and gently sonicated before being loaded into a CellASIC Y04C microfluidics plate (Milipore SIGMA) under continuous media flow at 2 psi ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were lysed in BufferD lysis buffer (10% glycerol, 125mM NaCl, 1mM EDTA, 1mM EGTA, pH 7.6) containing 1% TritonX and protease inhibitor cocktail (Millipore, 539131) for 1 hour at 4°C while rocking ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted in 100 μl extraction buffer (100 mM TRIS pH 7.5, 150cmM NaCl, 5 mM EDTA, 10% glycerol, 1x protease inhibitor cocktail (SIGMA, USA) and 1x phosphatase inhibitor (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... was added at equal volume and supplemented with 10 μL anti-fade solution (9 parts glycerol, 1 part 10X PBS, 0.1 part 20%(w/v) n-propyl gallate (Sigma P3130) in dimethyl sulfoxide) ...
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bioRxiv - Plant Biology 2021Quote: ... EE was gently removed and leaves were submerged in lactophenol trypan blue solution (5 ml of lactic acid, 10 ml of 50% glycerol, 1 mg of trypan blue (Sigma), and 5 ml of phenol ...
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bioRxiv - Cancer Biology 2020Quote: ... DUSP1 MISSION shRNA Bacterial Glycerol Stock (SHCLNG-NM_004417, total five constructs were tested TRCN0000367616, TRCN0000367617, TRCN0000231489, TRCN0000002517, TRCN0000002516) was purchased from Sigma Aldrich.
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bioRxiv - Cell Biology 2020Quote: ... This bacterial slurry was grown from 10 μL of a frozen glycerol stock incubated in 2 mL Luria-Bertani (LB) broth (Sigma) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... and buffer exchanged three times into protease buffer (20 mM Tris pH 8.0, 10% glycerol) using an Amicon Ultra centrifugal filter (Sigma-Aldrich). Purified His6-tagged TEV protease was added to all proteins at a 1:20 ratio (except for SpoVD ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies with BSA or glycerol contaminants were first concentrated in a 100 kDa MWCO spin column (EMD Millipore; cat. UFC510096) and washed twice with 400 μL of PBS before being transferred to the 50 kDa column ...
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bioRxiv - Biochemistry 2022Quote: ... Then 96 individual CFUs per biological replicate were resuspended in 100 µl of liquid YPD supplemented with 30% glycerol in a 96 well plate (NuncTM Sigma), as one colony/well ...
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bioRxiv - Cancer Biology 2021Quote: Cells were washed with cold DPBS for two times and then lysed in 2× Laemmli buffer (2% SDS, 20% glycerol, and 125 mM Tris-HCl, pH 6.8) supplemented with 1× protease inhibitor cocktail (Sigma, P8340). The cell lysate was scraped and sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... The buffer viral solution was exchanged with phosphate-buffered saline (PBS) plus 5% glycerol using an Amicon ultra-15 spin ultrafiltration (Millipore). Genome copies of final viral solutions were determined by qPCR using primers detecting WPRE and the shuttle plasmid as a standard ...
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bioRxiv - Physiology 2020Quote: ... Final triglyceride concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triglyceride Agent solution (Sigma Triglyceride and Free-Glycerol reagents ...
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bioRxiv - Neuroscience 2021Quote: ... the embryos were cleared in 50% glycerol and mounted dorsal side down on a glass bottom petri dish using low gelling agarose (Sigma). For live imaging ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM β-mercaptoethanol and 5% glycerol was mixed gently with 20 µL of 1 M dimethylamine-borane complex (Sigma) per 1 mL of protein solution ...
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bioRxiv - Genomics 2022Quote: ... Cells are then resuspended in 10ml Sucrose/Glycerol buffer (1:1) (Sucrose Buffer: 0.3M Sucrose; 1% Triton-X (Sigma Aldrich); 10mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Protein was buffer exchanged into phosphate buffered saline (PBS) containing 10% glycerol using a spin concentrator (10 kDa MWCO, Millipore).
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bioRxiv - Molecular Biology 2019Quote: Cell lysates were prepared with 125 mM Tris/HCl pH 6.8, 15% SDS, 10% Glycerol, 1.25 μM PMSF (Sigma-Aldrich, NSW, Australia and 1x protease inhibitor cocktail (Sigma-Aldrich) and sonicated 6 times (1 second pulses ...
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bioRxiv - Immunology 2020Quote: ... lysed with lysis buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 10% glycerol, 1% NP-40, protease inhibitor cocktail (Sigma Aldrich) and phosphatase inhibitor (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... Next the slides were mounted with a coverslip using Mowiol (6 g glycerol, 2.4 gr polyvinylalcohol 4-88 (Sigma, 81381), 6 ml MQ and 12 ml 0.2 M Tris HCL pH 8.5).
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bioRxiv - Plant Biology 2021Quote: ... 10%glycerol, 1 mM DTT, 1 mM EDTA, 0.5% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Insoluble debris was pelleted by centrifugation for 20 min with 4000 rpm at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... dipped in MilliQ, and mounted in Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Calbiochem, Merck Millipore, Darmstadt, Germany)) ...
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bioRxiv - Immunology 2021Quote: ... Aliquots of BCG cultures at an OD600 of 0.8 were stored at −80°C in RPMI-1640 media supplemented with 10% glycerol (Sigma-Aldrich). The number of viable bacteria was assessed by thawing a frozen aliquot and plating serial dilutions onto Middlebrook 7H11 (BD Pharmingen ...
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bioRxiv - Microbiology 2021Quote: Cells obtained from overnight cultures grown in YPD broth at 37°C were spotted in serial 10-fold dilutions on YP plates containing 2% glycerol or YPD plates containing 0.05 mg/ml of Congo red (Millipore Sigma) or 0.01 mg/ml of calcofluor white (Millipore Sigma) ...