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Citations for Millipore Sigma :
1051 - 1100 of 1404 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 10% glycerol and supplemented with Halt Protease Inhibitor Cocktail (Thermo #78430) and Benzonase (Millipore #70746-3). Cells were incubated at 4C shaking for at least 1 hour ...
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bioRxiv - Microbiology 2024Quote: Bacteria from lung homogenates were enumerated by plating on cetrimide + 1 % glycerol agar plates (Sigma-Aldrich) and incubated overnight in 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... We injected each mouse with 100 μg/kg of toxin (Takeoka and Arber, 2019) diluted in ultrapure water (Millipore, TMS-006-C) and let the animals in a confined cage for three days ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cholera toxin B-subunit (CTB; C9903) and heat labile enterotoxin B subunit (LTB; E9656) of Escherichia coli were purchased from Sigma (Sigma Aldrich). PhenoVue Fluor 594-WGA was obtained from PerkinElmer (Perkin Elmer Health Science Inc ...
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bioRxiv - Neuroscience 2023Quote: ... We used the same protocol described in Golub’s study.29 Adult mice received two intramuscular injections of diphtheria toxin (Sigma-Aldrich D0564-1MG) at 50 ng/g ...
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bioRxiv - Immunology 2024Quote: ... For pDC depletion mixed BM chimeras of 50% BDCA2- DTR and 50% either WT or LDHB-/- BM were treated with diphtheria toxin (DT) (Sigma- Aldrich, D0564) as described60 ...
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bioRxiv - Microbiology 2022Quote: ... Samples containing ∼1000 eggs per condition were mounted on glass slides with glycerol mounting medium (Sigma-Aldrich) and analyzed using a Zeiss Axio Observer at 400X with oil emersion for color images or EVOS FL Auto (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM EDTA, 10% glycerol, 0.5% IGEPAL CA-630, 0.25% Triton X-100 with 5µl/ml Sigma 8340 protease inhibitor cocktail incubated on ice 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 10% glycerol) supplemented with 0.02 μg / μl BSA and 0.1 μg / μl of Poly DI-DC (Sigma) in presence of serial dilution of purified BlpR/BlpS or buffer ...
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bioRxiv - Microbiology 2021Quote: ... and stored at 80°C in tryptic soy broth with 50% glycerol (Sigma-Aldrich, St. Louis, MO) until the time of use.
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bioRxiv - Cell Biology 2020Quote: ... A standard curve was produced by using Glycerol Standard Solution (Sigma-Aldrich, St. Louis, USA, Catalog G7793). The plate is incubated at 37C for 5 minutes and then developed with a plate reader set to detect absorbance at 540 nm ...
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bioRxiv - Cancer Biology 2022Quote: ... a small of amount of glycerol stock was extracted and added to LB broth (Sigma Cat. #L3522) supplemented with the relevant antibiotic ...
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bioRxiv - Microbiology 2022Quote: ... All bacterial strains were stored at - 80°C for long term storage in 25% sterile glycerol (Sigma). All cloning and strains were performed in dh10b genotype cells (NEB ...
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bioRxiv - Microbiology 2020Quote: ... and 1% glycerol supplemented with 5mM Na3VO4 and 1mM PMSF) and acid-washed glass beads (Sigma-Aldrich). Samples were vortexed for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The washed cell pellet was lysed in 5 pellet volumes of lysis buffer (50mM Tris pH 7.4, 50mM NaCl, 10% glycerol, 1.5mM MgCl2, 0.5mM CaCl2, 0.5% NP-40, 1x SigmaFast protease inhibitor [Sigma] ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were resuspended in 0.7ml of swelling buffer supplemented with 10% glycerol and 0.5% Igepal CA630 (Sigma), and gently pipetted up and down 15 times using a p1000 tip ...
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bioRxiv - Pathology 2020Quote: ... Serum Triglyceride and serum free glycerol were quantified using Serum Triglyceride Determination Kit (Sigma-Aldrich, cat# TR0100) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and 10% glycerol) that was freshly supplemented with 10 μl each of protease inhibitor (Sigma) and phosphatase inhibitor (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% Glycerol) supplemented with 1 mM Phenylmethylsulfonyl fluoride (Carl Roth) and 5 mM β-Mercaptoethanol (Sigma Aldrich). The resuspended cells were disrupted by sonication with a Branson Digital Sonifier (Model 102C ...
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bioRxiv - Genetics 2020Quote: ... Vectors were individually picked from glycerol stocks of The RNAi Consortium library (TRC Mm1.0) (Sigma-Aldrich, MO), grown up on agar plates and combined before maxiprep DNA isolation ...
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bioRxiv - Microbiology 2020Quote: ... All bacterial strains were stored at −80°C for long-term storage in 15% sterile glycerol (Sigma). The genotypes of E ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 30 µl of each sample were subsequently added to 100 µl free glycerol reagent (F6428; Sigma, USA) in a clear 96-well plate and incubated for 5 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... A standard curve was produced by using Glycerol Standard Solution (Sigma-Aldrich, St. Louis, USA, Catalog G7793). The plate is incubated at 37C for 5 minutes and then developed with a plate reader set to detect absorbance at 540 nm ...
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bioRxiv - Immunology 2020Quote: E-liquid mixture of 70% propylene glycol and 30% glycerol and 6mg/mL nicotine (purchased from Sigma) without flavors or additives were used ...
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bioRxiv - Microbiology 2021Quote: ... and the reaction was initiated by the addition of 3.7 mM sn-glycerol-3-phosphate (Sigma Aldrich). The reduction of MTT at 570 nm was continuously monitored on a BMG LABTECH plate reader for 118 min at room temperature.
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bioRxiv - Microbiology 2020Quote: ... 6 ml of amniotic fluid was dissolved in 3 ml of glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
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bioRxiv - Microbiology 2021Quote: ... All bacterial strains were stored at −80°C for long term storage in 25% sterile glycerol (Sigma).
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bioRxiv - Physiology 2022Quote: ... 10μL of each sample at the different points were mixed with 160μL of glycerol reagent (Sigma, F6428) and read at an absorbance of 540nm for glycerol levels.
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bioRxiv - Physiology 2022Quote: ... the slides were mounted with 90% glycerol in PBS plus 2.5 mg/ml p-Phenylenediamine (Sigma-Aldrich) and sealed with clear nail polish ...
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bioRxiv - Physiology 2023Quote: ... The triglycerides were determined enzymatically using Serum Triglyceride Determination Kit (Sigma TR0100; Glycerol Standard Solution Sigma G7793)(22).
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bioRxiv - Microbiology 2023Quote: ... All bacterial strains were stored at −80 °C for long-term storage in 15% sterile glycerol (Sigma). The genotype of E ...
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bioRxiv - Cancer Biology 2023Quote: ... 5% glycerol) using the Amicon Ultra-15 centrifugal filter column with a 10 kDa pore size (Millipore). Protein was quantified with the Bradford method before adjusting to a stock concentration of 4 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were mounted in glycerol / n-propyl gallate medium (4% n-propyl gallate (02370, Sigma-Aldrich), 8 ml 100% glycerol ...
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bioRxiv - Physiology 2023Quote: ... 5X SDS sample buffer (250 µM Tris/HCl, pH 7.0; 40% (v/v) glycerol (Sigma-Aldrich #G5516); 8% (w/v ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed in 1X PBS for 5 min and coverslipped with mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant was decanted and the pellet was resuspended in 0.25% (vol/vol) Glycerol (cat#: G5516, Sigma Aldrich) and 5% (wt/vol ...
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bioRxiv - Immunology 2023Quote: ... and cover slips were placed with mounting media (50% glycerol, [Sigma] and 4% propyl gallate [Sigma, #2370]).
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bioRxiv - Microbiology 2023Quote: ... the glycerol content of the extract was determined either using the Glycerin-Assay-Kit (MAK117, Sigma USA) or the Free Glycerol Determination Kit (FG0100 ...
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bioRxiv - Microbiology 2023Quote: ... 5% glycerol and 20 mM imidazole (binding buffer) in the presence of protease inhibitor cocktail (Sigma-Aldrich). Cells were lysed using an Emulsiflex B15 high pressure disruptor (Avestin ...
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bioRxiv - Biochemistry 2023Quote: ... The putative headgroups sn-glycerol-3-phosphate bis (cyclohexyl ammonium) salt (G3P) was also purchased from Sigma. Tris base was purchased from Fisher Bioreagents ...
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bioRxiv - Immunology 2023Quote: ... and cover slips were placed with mounting media (50% glycerol, [Sigma] and 4% propyl gallate [Sigma, #2370]).
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bioRxiv - Microbiology 2023Quote: ... and were bathed in anti-fading buffer (1x PBS, 60% glycerol, 0.2% propyl gallate (Sigma-Aldrich P3130)) instead of STORM buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentiviral shRNA constructs in the pLKO.1-puro vector were purchased as glycerol stocks from Millipore Sigma. For shADAR1 ...
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bioRxiv - Microbiology 2024Quote: Individual bacteria from glycerol stock were incubated on 1/2 tryptic soy broth (TSB, Sigma-Aldrich, USA) agar plates at 25°C for 5 days ...
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bioRxiv - Cell Biology 2023Quote: DRMs distribution on the cell surface was assessed by staining the cells with cholera-toxin coupled to FITC (CT-FITC, Sigma-Aldrich, Burlington, MA, USA). The target of cholera toxin is GM1 (monosialotetrahexosylganglioside) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL epidermal growth factor (PreproTech, Catalog No. AF-100-15) and 10−10 M cholera toxin (Sigma-Aldrich, Catalog No. C8052-5MG). VdH15 cells were grown in KSFM medium supplemented with 25 μg/mL BPE and 0.5 ng/ml epidermal growth factor ...
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bioRxiv - Biophysics 2021Quote: ... 20% glycerol, pH 7.4) into SEC Buffer 2 (50 mM Tris-HCl, 200 mM NaCl, 0.06% digitonin (Millipore), pH 7.4) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed 5X in 1% BSA and mounted by washing 3X in 80% glycerol (Millipore Sigma, #G5516) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 25 mM Tris pH7.4, 5 mM EDTA, 0.5 mM DTT, 1% NP40, 5% glycerol and protease inhibitor cocktail from Sigma). Cells were lysed using a Bioruptor (Diagenode ...