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Citations for Millipore Sigma :
1101 - 1150 of 1404 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Cross sections were mounted on glass slides in 50% glycerol (Sigma-Aldrich, Milwaukee, WI, USA, Cat# G7757-1GA) under cover slips.
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bioRxiv - Plant Biology 2019Quote: ... The pellet was washed and resuspended in 1ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% Tween 20 (Sigma)) and pelleted again by centrifugation as before ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples having a volume of 10 µL were removed at various time points and quenched with 5 µL of 3x loading buffer (5% Ficoll-type 400, 25% glycerol, 1.5X TBE, 20 mM EDTA, 0.5% SDS, 0.1% NP40 (Sigma 74385) and 160x excess of capture oligo ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.5, 150 mM NaCl, 1mM EDTA, 0.1% Nonidet P-40, 10% glycerol, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...
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bioRxiv - Genetics 2019Quote: ... Seedlings were harvested and ground in liquid nitrogen and total protein was extracted in IP buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 10% glycerol, supplemented with 5 mM DTT, 0.5 mM PMSF, Sigma protease inhibitor cocktail P9599 ...
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bioRxiv - Genetics 2021Quote: ... Complexes were diluted to 2.5uM in a protein storage buffer composed of 50% (v/v) glycerol (Sigma G5516), 100 mM NaCl (Fisher Scientific S271-3) ...
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bioRxiv - Neuroscience 2021Quote: ... was administered dissolved in identical ingredients (sodium chloride and glycerol) as in previous experiments using OXT nasal spray and administered sterile (0.22μm Millipore filtered - six 0.1ml puffs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... We prepared a saponified neutralized liver extract and created a standard curve with glycerol standard solution (Sigma G7793). Triolein content was assessed using free glycerol reagent (Sigma F6428) ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was washed and resuspended in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% Tween 20 (Sigma)) and pelleted again by centrifugation as before ...
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bioRxiv - Microbiology 2021Quote: Cellular proteins were extracted with a whole-cell extract buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, 1 mM EDTA, protease inhibitor cocktail [Sigma]). Cells lysates were resolved on 4–12% Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Lysis Buffer (150mM NaCl, 50mM Tris-Hcl, pH7.5, 10% Glycerol, 0.5%NP-40, 1mM MgCl2, 1/1000 Benzonase (Sigma), Phosphatase (PPI ...
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bioRxiv - Cell Biology 2020Quote: ... 30μg of protein was dissolved in sample-loading buffer (5% SDS, 0.2% bromophenol blue, 50% glycerol and 250mM Tris HCl pH8.5, all from Sigma), loaded onto 10% SDS-polyacrylamide gels and subsequently transferred onto nitrocellulose membranes (Protran ...
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bioRxiv - Biochemistry 2021Quote: ... The washed nuclear pellet was resuspended in one original cell pellet volume of NRB buffer (20mM HEPES pH 7.5, 75mM NaCl, 0.5mM EDTA, 50% glycerol, 1mM DTT, 1x SigmaFast Protease inhibitor [Sigma]). An equal volume of NUN buffer (20mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... 10 % Glycerol and 0.5 mM TCEP) in a D-Tube™ Dialyzer Maxi (EMD Millipore Corp, Billerica, USA) to remove traces of 2-mercaptoethanol as the reducing agent strongly inhibits the coupling to maleimide linked dyes to cysteines ...
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bioRxiv - Microbiology 2019Quote: ... cholerae hosts and prepped via polyethylene glycerol precipitation or concentration and media exchange on Amicon Ultra – 15 (Millipore) centrifugal filters (73 ...
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bioRxiv - Molecular Biology 2020Quote: ... We mixed the Tn5 transposase with annealed ME-Adaptor A or B (20μM) in 45% glycerol (Sigma, Cat.No.G5516) thoroughly and incubated the mixture at 30°C for one hour ...
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bioRxiv - Cell Biology 2021Quote: Serum glycerol and true triglyceride (TG) levels were determined using a Serum Triglyceride Determination Kit (Sigma-Aldrich TR0100). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... DPSCs were cultured in the mineralization-inducing fluid (5% FBS, 10 mmol/L β-sodium glycerol phosphate, Sigma; 10−7mol/L dexamethasone ...
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bioRxiv - Microbiology 2020Quote: ... medium and were grown in Middlebrook 7H9 broth (Becton Dickinson) supplemented with 10 % OADC (Himedia FD329) filtered 0.2% glycerol (SRL) and 0.05% Tween 80 (Sigma) at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was resuspended in buffer C (40 mM HEPES pH 7.6, 4 mM MgCl2, 0.6% Triton X-100, 0.5% IGEPAL CA-630, 20% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was resuspended and incubated for 30 min at 4 °C in buffer B (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were shortly rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore). Samples were imaged directly or within the next 48h ...
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bioRxiv - Systems Biology 2023Quote: ... 10% Glycerol) with 1 mM DTT and 1% Protease Inhibitor Cocktail (Millipore-Sigma, Burlington, MA, USA, Cat. # P8340), and extracts were sonicated 3x for 10 seconds each time (BRANSON Sonifier 150 ...
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bioRxiv - Genomics 2023Quote: ... Slide that now contained stained cells was air dried and mounted with 1ml of 85% glycerol (Merck Millipore) and covered with a coverslip (Menzel-Glaser) ...
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bioRxiv - Neuroscience 2023Quote: ... which contained glycerol and was purified before metal conjugation with Amicon 10K Buffer Exchange Columns (UFC501096, EMD Millipore). All antibody concentrations were obtained using a Nanodrop 2000c Spectrophotometer and formulated with a final stock concentration of 0.5mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... After processing sections were mounted on microscope slides with Tris-glycerol supplemented with 10% Mowiol (Merck Millipore Calbiochem).
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bioRxiv - Microbiology 2023Quote: ... theta (1 x 108 CFU) while control mice were orally administered vehicle (10% glycerol in Millipore-filtered water). Mice received three additional treatments at −2 ...
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bioRxiv - Immunology 2023Quote: ... the glass coverslips were mounted cell-side down on clean plain glass slides with 80% glycerol (Sigma, #G5516) and imaged using a CSU-W1 confocal microscope (Olympus).
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bioRxiv - Biochemistry 2022Quote: ... 500 μL 1 M MgSO4) supplemented with 0.5% glycerol and 0.05% (v/v) tyloxapol (Ty, Sigma, cat. #T0307) with appropriate antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed in 1xPBS for 5 minutes and coverslipped with 75µl of mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The reactions were then incubated at 37 °C for 60 min and stopped by adding 1 µl of stop buffer (0.4% SDS, 30 mM EDTA, and 6% glycerol) and 1µl of proteinase K (20 mg/ml, Sigma) into the reaction for another 10 min incubation at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... were diluted in Laemmli buffer (62.5 mM Tris, pH 6.8, 1.5% SDS, 8.33% glycerol [Fisher Scientific, BP2291], 1.5% β-mercaptoethanol [Sigma-Aldrich, M3148] ...
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bioRxiv - Biochemistry 2023Quote: ... 10 % (v/v) glycerol) by using a D-Tube Dialyzer with MWCO 6-8 kDa (Millipore, #71507-M) for 16 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM MgCl2, 0.5 mM EDTA, 0.5 mM DTT, 4% glycerol, 10 µg/ml of poly dI-dC [Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... 4% SDS and 20% de glycerol) supplemented with PMSF (1 mM) and a Protease Inhibitor Cocktail (PIC, Sigma). After vigorous vortexing ...
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bioRxiv - Microbiology 2022Quote: Crude bacterial template DNA for toxin profiling was prepared by resuspension of cells in a 5% (wt/vol) solution of Chelex-100 resin (Sigma-Aldrich, Castle Hill, NSW, Australia). All isolates were screened by PCR for the presence of toxin A (tcdA) ...
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bioRxiv - Cancer Biology 2021Quote: ... Counterstaining of nucleus was performed using Mayer’s hemalum solution and slides were mounted with Kaiser’s glycerol gelatin (the Kit and all reagents were obtained from Merck Millipore). Images were acquired as described above.
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bioRxiv - Cell Biology 2020Quote: Organs from 6 weeks old wild-type C57BL/6 mice and FLAG-RIPK2 knock-in mice were lysed in IP buffer (150 mM NaCl, 50 mM Tris pH 7.5, 10% glycerol, 1% Triton X-100, 2 mM EDTA, all from SIGMA) supplemented with protease inhibitors (cOmplete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted and resuspended in Buffer A (50 mM Tris pH 8.0, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, 10% glycerol, all from SIGMA) and sonicated ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 20-30 column volumes wash buffer (PBS, 10% glycerol and 2mM PMSF) at 0.5 ml/min and eluted with 50mM glutathione (SIGMA) (wash buffer supplemented with 50mM glutathione) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 µl of total RNA (2.5µg/µl) were added to 20 µl of “glyoxal mix” (60% DMSO, BTPE 1.2X, 5% glycerol, 8% glyoxal solution (Sigma) and 40 µg/ml Ethidium bromide) ...
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bioRxiv - Developmental Biology 2022Quote: ... lysed them in NP40 lysis buffer (50mMTris-HCl, 150mMNaCl, 0.1% NP-40, 5mM EDTA, 10% glycerol) with protease inhibitors cocktail (Sigma), and sonicated using a Bioruptor (Diagenode) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The pellet was then re-suspended in 1 mL of fresh M9 media and 1 mL of glycerol (Sigma) 20% to a final glycerol concentration of 10% ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were lysed in modified Laemmli buffer (5% SDS, 10% glycerol, 32.9 mM Tris-HCl pH 6.8) supplemented with 1 mM DTT (Sigma-Aldrich), and their extracts (15 µG ...
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bioRxiv - Developmental Biology 2019Quote: Protein extracts from cells were prepared using a modified Laemmli buffer (5% sodium dodecyl sulphate, 10% glycerol, 32.9 mM Tris-HCl pH 6.8) supplemented with protease inhibitors (Sigma-Aldrich). Brain tissues were prepared with a lysis buffer (Hepes 10 mM ph 7.9 ...
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bioRxiv - Plant Biology 2020Quote: ... and ground in liquid N2 in 2 volumes of extraction buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Nonidet P-40 and 1x protease inhibitor cocktail; Sigma-Aldrich) and centrifuged for 12,000 g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... to assess protein concentration and then solubilized in 4x loading dye (250 mM Tris-HCl pH 6.8, 40% glycerol, 0.008% bromophenol blue, 8% SDS; all from Sigma-Aldrich). All samples were boiled at 65°C for 10 minutes and then equal amounts of each sample were separated using SDS-PAGE and subsequently blotted on nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was discarded and the membrane fractions were homogenized in 20 mL solubilization buffer (25 mM HEPES pH 7.5, 150 mM NaCl, 5% v/v glycerol) with cocktail inhibitors (Sigma) per initial liter of culture volume ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% Glycerol) containing a 3-fold dilutions (from 0.125 units/ml to 6 units/ml) of Benzonase nuclease (Millipore). This was mixed gently and incubated for three minutes at 37°C ...