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Citations for Millipore Sigma :
1251 - 1300 of 7955 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Commercial primary antibodies were purchased for the detection of TIGAR (AB10545, Millipore/Sigma-Aldrich), ChAT (703789 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Detection was performed with peroxidase-conjugated anti-mouse IgG 1:5000 (Sigma, MO, USA) and anti-human IgG 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Physiology 2022Quote: ... Bound antibodies were detected using a chemiluminescence HRP substrate detection solution (Millipore, Watford, UK). Band quantification was determined using BioRad Image Lab Software.
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bioRxiv - Immunology 2022Quote: ... Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore, Bedford, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: PLA in cultured PAEC were performed using Duolink PLA protein detection technology (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... oligonucleotides for detection of mRNA abundance and SYBER Green JumpStart Taq Ready Mix (Sigma). Three-step protocol runs were carried out in a Rotor-Gene Q instrument (QIAGEN) ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used for detection: anti-FMRP (MAB2160, Millipore; 6B8, Biolegend), and anti-Calnexin (Sigma ...
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bioRxiv - Immunology 2020Quote: ... This was followed by species-specific detection HRP-conjugated antibodies (1/5000; Millipore, USA). Protein bands were detected using SuperSignal Chemiluminescent HRP substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection was carried out with the Immobilon Crescendo Western HRP substrate (Merck Millipore®), using the Chemidoc imaging system and the Image Lab software (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for detection: HRP-conjugated anti-Flag antibody (Sigma, A8592), rabbit anti-GFP (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, catalog no. WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: PLA in cultured PAEC were performed using Duolink PLA protein detection technology (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the manufactory’s instruction was followed with Duolink® In Situ Detection Reagents (Sigma-Aldrich). The interaction between proteins was found using Image J software.
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bioRxiv - Molecular Biology 2024Quote: PLA experiments were performed using Duolink® In Situ Detection assay from Sigma-Aldrich according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Simultaneous detection was performed by incubating with both 1:2,000 anti-dig-AP (Sigma) and 1:2,000 anti-fluorescein-POD (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... using rabbit PLAplus and mouse PLAminus probes and red Duolink detection reagents (Sigma Aldrich). Before mounting with Duolink in situ mounting medium with DAPI (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection of the nucleus was done with the Hoechst (1/1000, Sigma-Aldrich®). Slides were counterstained with Hoechst (1/1000 ...
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bioRxiv - Plant Biology 2023Quote: ... Immuno-positive bands were detected after the reaction with the detection reagents NBT (Sigma) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate ...
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bioRxiv - Cancer Biology 2024Quote: ... For detection it was used primary anti-NT5C2 antibody (3C1 Clone, WH0022978M2 Sigma-Aldrich), anti-FPGS antibody (Clone EPR11064 ...
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bioRxiv - Plant Biology 2021Quote: ... and in vitro crosslinked with 1% formaldehyde (20 minutes at RT; Sigma, Cat# F8775). The chromatin was then fragmented to ∼500bp by sonication ...
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bioRxiv - Physiology 2019Quote: ... frozen muscle powder was homogenised using RNAzol RT reagent (Sigma-Aldrich, St Louis, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The plate was immediately covered with a silicone sealing film (ThermalSeal RTS, Sigma-Aldrich), and incubated in a plate reader (CLARIOstar or FLUOstar Omega ...
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bioRxiv - Bioengineering 2020Quote: ... for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich) for 5-10 min ...
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bioRxiv - Microbiology 2020Quote: ... for 2 hours at RT and mounting with aqueous mounting medium (F4680, Sigma Aldrich). For sheep cells imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma)/1ml PBS for 30 minutes at RT and nuclei were stained with DAPI (Sigma) at RT for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... for 1h at RT and developed using Immobilon(tm) Western Chemiluminescent HRP Substrate (Millipore). Detection and quantification of chemiluminescence intensities were quantified by using Chemidoc™ imaging system ...
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bioRxiv - Cell Biology 2021Quote: ... Serum blocking was performed for 20min at RT in 1xWBR (Western Blocking Reagent) (Sigma) solution ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at RT in anti-TH (1:1000; T2928, Millipore), followed by incubation in anti-mouse biotinylated antibody (1:300 ...
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bioRxiv - Pathology 2022Quote: ... Filtered samples (100µL) were incubated (1h; RT) with MOPS buffer (5mM, 100µL, Sigma, #M1254), Bathophenanthrolinedisulfonic acid disodium salt hydrate (BPT ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were washed for 15 minutes at RT with 1xPBS/0.1% TritonX 100 (Sigma)/0.1 M Sodium Acetate pH6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were further treated 15 minutes at RT with 1ug/mL Proteinase K (Sigma) followed by four 5 minutes washes in 1xPBT ...
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bioRxiv - Molecular Biology 2020Quote: ... #: 01813-F) for 10 minutes at RT before fixing with 4% glutaraldehyde (Sigma Aldrich) and contrasting with 2% phosphotungstic acid (Sigma Aldrich) ...
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bioRxiv - Pathology 2019Quote: Total RNA was extracted from snap-frozen tissues using RNAzol RT (R4533, Sigma-Aldrich) followed by Purelink RNA mini kit (12183025 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 hour blocking was performed at RT using 5% Bovine Serum Albumin (BSA, Sigma) in PBS-T (PBS with 0.1% Triton-X) ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... RT-qPCR was carried out as previously described using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes [42] ...
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bioRxiv - Physiology 2020Quote: Frozen muscle powder was homogenized using RNAzol RT reagent (Sigma-Aldrich, St Louis, MO) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Sigma #D9542, 1:1000 for 10min at RT) Mice were deeply anesthetized with isoflurane and then transcardially perfused with ice-cold heparinized PBS (25U/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... at RT for 2 h and BM purple alkaline phosphatase substrate (Sigma-Aldrich, 1144207001) was used to visualize the color in the humid chamber ...
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bioRxiv - Bioengineering 2022Quote: ... antigen retrieval was done with Pronase (1mg/mL for 10 minutes at RT, Sigma) followed by blocking with BSA (3% w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Blocking was performed at RT with 2% bovine serum album (BSA) (Sigma-Aldrich, #A7906), 2% goat serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: RT-qPCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Supplementary Table 4) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 20 min at RT and permeabilised in permeabilization buffer [1% BSA (Sigma Aldrich) and 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS (PBST) at RT followed by blocking solution (PBST + 4% BSA (Sigma A9647)) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed for 10 minutes at RT with 4% formaldehyde (Merck, Sigma Aldrich) diluted in PBS 1X (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... for 10 min at RT and blocked with 1% BSA (Sigma-Aldrich, MA, USA) in PBS for 30 min at RT ...
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bioRxiv - Immunology 2022Quote: ... the cells suspension was reconstituted in RT CV medium/10μM Y-27632 (Sigma Aldrich) and 150’000 cells/cm2 were added to the prepared collagen I hydrogels ...
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bioRxiv - Microbiology 2023Quote: ... for 60 min at RT and alkylated with 40 mM iodoacetamide (IAA, Sigma-Aldrich) in the dark for at least 60 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... for an hour at RT after permeabilization with 0.2% Triton X-100 (Sigma-Aldrich) in PBS for 35 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilised for 20 min at RT using PBS/0.25% Triton X-100 (Sigma). Cells were blocked for 30 min at RT with blocking solution (PBS-0.25% Triton X-100 plus BSA 1%) ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min at RT and permeabilized in 0.25% Triton X-100 (Sigma-Aldrich) for 20 min at RT ...