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Citations for Millipore Sigma :
1201 - 1250 of 7557 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich) for 5-10 min ...
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bioRxiv - Microbiology 2020Quote: ... for 2 hours at RT and mounting with aqueous mounting medium (F4680, Sigma Aldrich). For sheep cells imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma)/1ml PBS for 30 minutes at RT and nuclei were stained with DAPI (Sigma) at RT for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... for 1h at RT and developed using Immobilon(tm) Western Chemiluminescent HRP Substrate (Millipore). Detection and quantification of chemiluminescence intensities were quantified by using Chemidoc™ imaging system ...
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bioRxiv - Cell Biology 2021Quote: ... Serum blocking was performed for 20min at RT in 1xWBR (Western Blocking Reagent) (Sigma) solution ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at RT in anti-TH (1:1000; T2928, Millipore), followed by incubation in anti-mouse biotinylated antibody (1:300 ...
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bioRxiv - Pathology 2022Quote: ... Filtered samples (100µL) were incubated (1h; RT) with MOPS buffer (5mM, 100µL, Sigma, #M1254), Bathophenanthrolinedisulfonic acid disodium salt hydrate (BPT ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were washed for 15 minutes at RT with 1xPBS/0.1% TritonX 100 (Sigma)/0.1 M Sodium Acetate pH6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were further treated 15 minutes at RT with 1ug/mL Proteinase K (Sigma) followed by four 5 minutes washes in 1xPBT ...
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bioRxiv - Molecular Biology 2020Quote: ... #: 01813-F) for 10 minutes at RT before fixing with 4% glutaraldehyde (Sigma Aldrich) and contrasting with 2% phosphotungstic acid (Sigma Aldrich) ...
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bioRxiv - Pathology 2019Quote: Total RNA was extracted from snap-frozen tissues using RNAzol RT (R4533, Sigma-Aldrich) followed by Purelink RNA mini kit (12183025 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 hour blocking was performed at RT using 5% Bovine Serum Albumin (BSA, Sigma) in PBS-T (PBS with 0.1% Triton-X) ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... RT-qPCR was carried out as previously described using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes [42] ...
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bioRxiv - Physiology 2020Quote: Frozen muscle powder was homogenized using RNAzol RT reagent (Sigma-Aldrich, St Louis, MO) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Sigma #D9542, 1:1000 for 10min at RT) Mice were deeply anesthetized with isoflurane and then transcardially perfused with ice-cold heparinized PBS (25U/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... at RT for 2 h and BM purple alkaline phosphatase substrate (Sigma-Aldrich, 1144207001) was used to visualize the color in the humid chamber ...
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bioRxiv - Bioengineering 2022Quote: ... antigen retrieval was done with Pronase (1mg/mL for 10 minutes at RT, Sigma) followed by blocking with BSA (3% w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Blocking was performed at RT with 2% bovine serum album (BSA) (Sigma-Aldrich, #A7906), 2% goat serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: RT-qPCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Supplementary Table 4) ...
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bioRxiv - Immunology 2022Quote: ... the cells suspension was reconstituted in RT CV medium/10μM Y-27632 (Sigma Aldrich) and 150’000 cells/cm2 were added to the prepared collagen I hydrogels ...
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bioRxiv - Microbiology 2023Quote: ... for 60 min at RT and alkylated with 40 mM iodoacetamide (IAA, Sigma-Aldrich) in the dark for at least 60 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... for an hour at RT after permeabilization with 0.2% Triton X-100 (Sigma-Aldrich) in PBS for 35 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilised for 20 min at RT using PBS/0.25% Triton X-100 (Sigma). Cells were blocked for 30 min at RT with blocking solution (PBS-0.25% Triton X-100 plus BSA 1%) ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min at RT and permeabilized in 0.25% Triton X-100 (Sigma-Aldrich) for 20 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was cooled overnight at RT and filtered through 0.22 µm filter (Millipore). The insoluble PG sacculi were collected on the filters ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized for 1 h at RT in PBS + 0.3% Triton X-100 (Sigma-Aldrich) (PBST) ...
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bioRxiv - Physiology 2023Quote: ... for 1h at RT and developed using Immobilon™ Western Chemiluminescent HRP Substrate (Millipore). Detection and quantification of chemiluminescence intensities were quantified by using ChemidocTM imaging system and Image Lab 5.2.1 software (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... and (3) 3 hrs at RT in 0.1% Direct Red 23 (212490, Sigma-Aldrich), in PBST ...
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bioRxiv - Molecular Biology 2024Quote: ... for 20 min at RT and permeabilised in permeabilization buffer [1% BSA (Sigma Aldrich) and 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS (PBST) at RT followed by blocking solution (PBST + 4% BSA (Sigma A9647)) ...
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bioRxiv - Immunology 2024Quote: ... for 10 min at RT and blocked with 1% BSA (Sigma-Aldrich, MA, USA) in PBS for 30 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed for 10 minutes at RT with 4% formaldehyde (Merck, Sigma Aldrich) diluted in PBS 1X (Gibco) ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich), Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Microbiology 2019Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich) or with the Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed using REDTaq® ReadyMix™ PCR Reaction Mix (R2523, Merck-SIGMA) using previously published primers (Charalambous et al ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Systems Biology 2022Quote: ... and cDNA fragments were amplified by PCR according to the manufacturer’s protocol (GenomePlex Single Cell Whole Genome Amplification Kit, Sigma Aldrich WGA4). The amplified libraries were run on a bioanalyzer to assess quality (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
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bioRxiv - Pathology 2022Quote: ... Pf2-HA_OE and pf2-HA_KO ChIP and input control samples) was recovered from Proteinase K treated samples using the GenElute PCR purification kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... or ear biopsies (3 week old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was then performed using 200 ng of 4C-template DNA with the Expand Long Range PCR kit (Millipore, 4829042001) under specific PCR conditions (94 °C for 2 min ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR1 products for each tumor were individually column-purified with a GenElute™ PCR Clean-Up Kit (Sigma-Aldrich NA1020-1KT). For PCR2 ...
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bioRxiv - Cell Biology 2023Quote: ... elegans genomic DNA extracted from 8-10 adult worms using the Extract-N-Amp™ Tissue PCR Kit (Millipore Sigma, MA) as previously described38 then sequenced ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from mice ear tissues (or tail for embryos) using Extraction « Extract-N-Amp PCR » kit (Sigma #XNAT2-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR sex genotyping reactions were performed in a final volume of 25 μl with the following reagents and volumes per sample: 12.5 μl KAPA2G Fast HotStart DNA Polymerase (5 U/μL) from the KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, KK5601), 1 μl of 12.5 μM Ube forward primer and 1 μl of 12.5 μM Ube reserve primer ...
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bioRxiv - Biochemistry 2024Quote: ... R-domain phosphorylation sites and interacting residues mutants were generated by site-directed mutagenesis using a Q5-New England Biolabs PCR mutagenesis kit together with primers synthesized from Millipore sigma and verified by sequencing (Elim Biopharmaceuticals ...