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Citations for Millipore Sigma :
1151 - 1200 of 7955 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... RNA was crosslinked using UV light and hybridized with a DIG labelled probe (PCR DIG probe synthesis kit, Sigma). For detection of LbuCas13a the membrane was stripped and probed with DIG labelled Cas13a specific probe and signal detected on a Licor Odyssey imaging system (LI-COR Bioscience ...
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bioRxiv - Developmental Biology 2020Quote: Genomic DNA extractions were performed with Extract-N-Amp or REDExtract-N-Amp™ Tissue PCR Kits (Sigma-Aldrich). Whole embryos or adult zebrafish fin-clips were lysed by incubation in a mixture of 50 μL extraction solution (Extraction Solution E7526 ...
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bioRxiv - Zoology 2020Quote: PCR amplification of two genomic regions were performed using 12.5 μl of the Extract-N-AmpTM Tissue PCR kit (Sigma), 1 μl of each primer ...
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bioRxiv - Immunology 2021Quote: ... The PCR products were used to synthesize dsRNA using a T7 RNA Polymerase Kit (Sigma-Aldrich RPOLT7-RO ROCHE). Generated dsRNAs were treated with TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... and pinx1 were generated from pCRII-TOPO vectors or PCR products using a digoxigenin RNA labeling kit (Sigma-Aldrich). For Supplementary Fig ...
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bioRxiv - Biochemistry 2023Quote: Amplified genes were cloned into pJET1.2 vector with the CloneJET PCR Cloning Kit (ThermoScientific) and further subcloned into pET30a and pET-DUET1 (Novagen) for protein expression in E ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from mouse tail tip samples using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR was performed using the REDExtract-N-Amp PCR Readymix (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... we deployed a rapid lysis protocol using reagents from the SYBR Green Extract-N-Amp Tissue PCR Kit (Sigma) (Doan and Monuki ...
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bioRxiv - Molecular Biology 2024Quote: ... The presence/absence of receptor–receptor molecular interactions in the samples was detected using the Duolink II in Situ PLA Detection Kit (developed by Olink Bioscience, Uppsala, Sweden; and now distributed by Sigma-Aldrich as Duolink®, using PLA® Technology).
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 2 times with 1X PBS and 1 time with MilliQ before mounting on microscope slides using Mowiol (10% Mowiol (Millipore, 475904), 0.1% diazabicyclo(2,2,2)-octane ...
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bioRxiv - Developmental Biology 2020Quote: ... the beads were washed 10 times with RIPA buffer then 5 times with 100 mM ammonium hydrogen carbonate (AMBIC) (Sigma-Aldrich) solution to remove salts and detergents ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 2 times with 1X PBS and 1 time with MilliQ before mounting them on microscope slides using Mowiol (10% Mowiol (Millipore, 475904), 0.1% diazabicyclo(2,2,2)-octane ...
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bioRxiv - Genomics 2022Quote: ... The tissue was homogenized with 10 times of slow douncing using a loose pestle and 15 times of douncing using a tight pestle (D9063, Sigma-Aldrich). The homogenate was filtered through a 40-μm cell strainer (22363547 ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR was performed using the Red Taq Ready PCR system (Sigma Aldrich) using primers described in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed using the REDExtract-N-Amp PCR Readymix (Sigma-Aldrich). The following primers were used for genotyping ...
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bioRxiv - Plant Biology 2020Quote: Detection of H2O2 was carried out using DAB (3,3-diaminobenzidine, Sigma-Aldrich, Switzerland) staining as described (Thordal - Christensen et al ...
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bioRxiv - Molecular Biology 2021Quote: ... Localized rolling circle amplification was performed using Detection Reagents Red (DUO92008, Sigma Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by detection with a goat anti-mouse IgG HRP (1/1000; Millipore) for 1h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP Substrate (Millipore) using a Gel-Doc XR+ System (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... for SARS CoV-2 spike detection mouse anti-spike (Cat# ZMS1076, Sigma Aldrich) respectively ...
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bioRxiv - Cell Biology 2021Quote: ... The primary antibody for fluorescence detection of ssDNA was mouse anti-ssDNA (Millipore). The secondary antibody was donkey anti-mouse Alexa Fluor 647 (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... For HRP detection (Fusion FX, Vilber Lourmat) chemiluminescent substrate Immobilon Classico (Merck Millipore) was used ...
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bioRxiv - Immunology 2019Quote: ... This was followed by incubation with Chemiluminescent HRP detection reagent (Merck Millipore, #WBKLS0500) for 1 min before image acquisition.
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bioRxiv - Neuroscience 2022Quote: ... Blots were developed with the ECL Prime Western Blotting Detection Reagents (Sigma-Aldrich) and bands imaged and quantified using the ChemiDoc system (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: In situ ROS detection was carried out using dihydroethidium (DHE) (Sigma-Aldrich, Israel). Briefly ...
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bioRxiv - Neuroscience 2024Quote: Following the protocol of the Duolink In Situ Detection Reagents Orange (Sigma, DUO92007), PLA experiments were also conducted ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cell Biology 2024Quote: ... The structure parameter and detection volume were calibrated using Rhodamine-6G (Sigma-Aldrich) solution with diffusion coefficient (280 μm2/s ...
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bioRxiv - Developmental Biology 2024Quote: ... Duolink Proximity Ligation Assay In Situ Detection Reagents (Sigma-Aldrich DUO92002, DUO92004, DUO92008) were used according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... To perform the PLA assay the Duolink In Situ Detection reagents (Sigma Aldrich) were used ...
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bioRxiv - Microbiology 2023Quote: ... Detection by ECL was carried out using Immobilon Western Chemiluminescent HRP Substrate (Millipore).
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the FLAG tag (A8592, Sigma-Aldrich, clone M2, monoclonal), PAP (P1291 ...
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bioRxiv - Microbiology 2023Quote: ... Detection was performed at 210 nm and analytical standards (Sigma-Aldrich Co., USA) were used for quantification by external calibration curves.
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Ligations and amplifications were performed with In Situ Detection Reagent Red (Sigma Aldrich), stained for DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (ECL, EMD Millipore, #WBKLS0500) and the BioRad ChemiDoc MP (#12003153 ...
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bioRxiv - Microbiology 2023Quote: ... Signal was detected using Immobilon HRP chemiluminescence substrate detection reagent (Merck Millipore, Germany) and imaged in a chemiluminescent imager (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... peroxidase detection was conducted with 0.066% of 3,3-diaminobenzidine-tetrahydrochloride (DAB; Sigma-Aldrich), 0.03% NiCl2 and 0.06% H2O2 ...
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bioRxiv - Immunology 2024Quote: ... followed by detection with a goat anti-mouse IgG HRP (1/1000; Millipore) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification was performed using the ‘Duolink In Situ Detection Reagents FarRed’ (Sigma-Aldrich) at 37°C for 100 min ...
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bioRxiv - Microbiology 2021Quote: Nitrite concentration in the lung homogenate was measured (n=3 per group per time point) using Griess Reagent kit following manufacturer instructions (Sigma, St. Louis, MO, USA). Absorbance was recorded using a GloMax plate reader (Promega Corporation ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR-based (Sigma-Aldrich).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... All PCR primers (Sigma) were reconstituted in molecular grade water (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... All PCR primers (Sigma) are provided in Supplementary Table 1.
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bioRxiv - Cancer Biology 2019Quote: ... RT-qPCR was performed using SYBR-Green Mastermix with ROX reference dye (Sigma). A list of all primer sequences used can be found in Table 1 below ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were diluted 1:2 for RT-qPCR using SYBR Green Mastermix (Sigma) and primers detailed in Table 3.
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bioRxiv - Developmental Biology 2021Quote: ... and 2 hrs at RT in blocking buffer containing anti-HA antibody (Sigma). After rinsing with PBS ...
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bioRxiv - Physiology 2020Quote: ... Muscle powder was homogenized using RNAzol RT reagent (Sigma-Aldrich, St Louis, MO) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... at RT for 60 min and alkylated with 40 mM iodoacetamide (Sigma-Aldrich) at RT in the dark for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... blocked for 30 min at RT with 10% heat-inactivated goat serum (Sigma) in PBS−/− ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked for 1 hour at RT in a phosphoprotein blocker (Millipore) and probed for total (Cell Signaling ...