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Citations for Millipore Sigma :
1401 - 1450 of 7557 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The proximity of the interested molecules was visualized using Duolink In Situ Detection reagents (Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The DuoLink in Situ Detection reagents Orange (λex = 554 nm and λem = 576 nm; Millipore Sigma) was used to determine whether SMAD3 interacts with SP1 in THP-1 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... PLA DuoLink red or green detection reagents and DuoLink anti-mouse and anti-rabbit antibodies (Millipore-Sigma Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... both in blocking solution and detection using the Supersensitive TMB substrate (3,3’,5,5’-tetramethylbenzidine, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Wako Lab Chemicals) for 4 hours at RT before dilution with ammonium bicarbonate (20 mM; Sigma) and digestion with trypsin (1/50 (w/w) ...
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bioRxiv - Genetics 2019Quote: ... LK03) were pooled in equimolar ratio and fragmented at room temperature (RT) using DNAse I (Sigma). At different incubation time points aliquots were analyzed on an 1.5% agarose gel while the reaction was temporarily stopped by incubation in a dry ice / ethanol bath ...
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bioRxiv - Neuroscience 2020Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Microbiology 2020Quote: ... left 5 min at RT for cell attachment and fixed with 4% paraformaldehyde (PFA) (Sigma P6148) for 10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by RT-qPCR with the FastStart Universal SYBR Green Master (Sigma-Aldrich, Cat No. 4913850001). Primers are listed in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in 1.5% BSA for 1 hr at RT and incubated with 4% PFA (Sigma) for another 24 hr in case of SARS-CoV-2 infected cells ...
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bioRxiv - Biophysics 2020Quote: ... and then incubated for 10 min at RT with 25U benzonase nuclease (Millipore Sigma 70746-10KUN)/50 μL sample ...
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bioRxiv - Molecular Biology 2022Quote: RPA and RT-RPA reactions are performed on a variety of commercially available porous membranes (Millipore GF041 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... for 4 minutes at RT and sections were counterstained with haematoxylin (Sigma Chemie GmbH, Steinheim, Germany), dehydrated in ethanol and mounted with Pertex (Histolab ...
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bioRxiv - Cell Biology 2022Quote: Larval preparations were fixed for 20 min at room temperature (RT) with 4% formaldehyde (Sigma #47608) in 1x phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... after fixation of cells with 4% Formaldehyde (10 minutes at RT, Sigma Aldrich, St. Louis, USA). Images were acquired on a Cytation3 imaging reader (BioTek ...
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bioRxiv - Molecular Biology 2021Quote: ... and Ms1 and the m7G-capped control were quantified by RT-qPCR with SYBR Green (Sigma). The fraction of treatment-resistant Ms1 was calculated by dividing the amount of transcripts left after the enzymatic treatment (resistant ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS for 1 hour at RT and permeabilized in 1% Triton X-100 (Sigma, #T9284) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed in 3.7% formaldehyde for 20 minutes at RT and permeabilized in 1% BSA (Sigma-Aldrich) and 0.2% Triton X-100 (Sigma-Aldrich)-containing buffer for 10min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h in the dark at RT and counterstained with DAPI (1:1000, Sigma, T7024). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... Secondary antibodies were diluted similarly and incubated for 1 h at RT and DAPI (Sigma, D1306) staining for 4 minutes at RT was used for nuclear visualization ...
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bioRxiv - Biophysics 2023Quote: ... and treated for 2 min at RT with pepsin (40 units/mL; Sigma-Aldrich, cat. P6887) diluted in 10 mM HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were stained for 1 h at RT with antibodies against vinculin (hVin1, Sigma; 1:400) and either (phosphoTyr397-FAK (Cat# 44-625G ...
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bioRxiv - Developmental Biology 2023Quote: ... By incubating at RT in darkness for 1 hour in a 1% hydrogen peroxide (Sigma, H1009) solution in 40 % methanol in PBS ...
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bioRxiv - Immunology 2023Quote: ... Tissue was washed, incubated with DAPI for 5min at RT (1:5000, in PBS (Sigma Aldrich)) and washed ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed at RT for 15 min in 4 % PFA pH 7.4 (Sigma-Aldrich, #P6148-500G). After washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl RT mix was added (containing 25 mM Trizma hydrochloride solution pH 8 (Sigma Aldrich), 30 mM sodium chloride (Carl Roth) ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated for 1h at RT in blocking buffer containing 10% goat serum (Sigma, G9023) with 0.03 % triton X-100 in PBS and 1:50 Affini Pure Fab Fragment Donkey Anti-Mouse IgG (Jackson Immuno Research Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... in 950 µL PBS for 2 h at room temperature (RT) with Biotin-NHS (H1759, Sigma) in a 1:20 molar ratio (G-CSFR:Biotin-NHS) ...
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bioRxiv - Plant Biology 2024Quote: The gels were incubated 1.5h at RT under gentle shaking with NHS-ester ATTO488 (Sigma, 41698), NHS-ester ATTO647 (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... at RT and incubated overnight with a monoclonal Anti-FLAG antibody coupled to horseradish peroxidase (Sigma #A8592 ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (2X) Universal (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR reaction mixture contained 2.5 units Taq polymerase and 1 x PCR buffer (D1806, Sigma-Aldrich), 0.25 mM dNTP mix and 0.5 μM of each of the primers ...
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bioRxiv - Plant Biology 2020Quote: ... An equal concentration of cDNA from the samples comparing was used for qRT-PCR reaction with KAPA SYBR Fast qPCR Kit (Sigma-Aldrich, St. Louis, USA). For expression analysis of the genes responsive to nutrient starvation ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted at the field using quick Extract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich Co. LLC, MO, Louis. St; USA) with some modifications ...
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bioRxiv - Developmental Biology 2022Quote: ... offspring were screened for Akt1 or Foxo4 mutations from genomic DNA from tail-tip biopsies using the REDExtract-N-Amp™ Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA). PCR was performed on the purified DNA samples using primers flanking the guide RNA sites ...
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bioRxiv - Microbiology 2024Quote: PCR products obtained from the first round of amplification (COMPETE, BLOCK, CONTROL, see section 2.2) were purified using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, St. Louis, MO). The purified DNA was diluted 10-fold with DNA free sterile water and used as a DNA template for a nested PCR to attach Illumina adaptors for Illumina sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were washed several times in PBS before mounting using Fluoromount (Sigma).
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bioRxiv - Immunology 2020Quote: ... they were washed four times and 50 μl tetramethylbenzidine (TMB, Sigma-Aldrich) was added into each well ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed slowly five times with 500 µL 1X PBS (Sigma) and detached by addition of 100 µL of 0.25% trypsin-EDTA solution (Gibco ...
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bioRxiv - Genomics 2020Quote: ... PBMCs were washed three times and stained with propidium iodide (PI, Sigma). Viable cells (PI negative ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times in PBS and stained with Hematoxylin (Sigma, Darmstadt, Germany) for 1 min ...
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bioRxiv - Genomics 2019Quote: ... samples were washed three times with phosphate buffered saline (Sigma Aldrich, UK). After the final cleaning step 1 mL Trizol was added to the cells ...
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bioRxiv - Developmental Biology 2019Quote: ... washed 3 times in PBS and incubated in DAPI (1:1000, Sigma) for 20 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... cells were gently washed 3 times with DPBS (Sigma, St Louis, MO). Finally ...
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bioRxiv - Molecular Biology 2019Quote: ... dialyzed three times against 4 L of 2 mM β-mercaptoethanol (Sigma) in water ...
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bioRxiv - Biophysics 2020Quote: ... the chambers were washed three times with PBS (Sigma-Aldrich, D8537-500ML) before incubation with 0.1 g/l Neutravidin (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... After washing the chambers three times with PBS (Sigma-Aldrich, D8537-500ML), the surfaces were incubated with 1 M KOH (Fulka ...