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Citations for Millipore Sigma :
1501 - 1550 of 7955 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... at RT and incubated overnight with a monoclonal Anti-FLAG antibody coupled to horseradish peroxidase (Sigma #A8592 ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (2X) Universal (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed in 3.7% formaldehyde for 20 minutes at RT and permeabilized in 1% BSA (Sigma-Aldrich) and 0.2% Triton X-100 (Sigma-Aldrich)-containing buffer for 10min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h in the dark at RT and counterstained with DAPI (1:1000, Sigma, T7024). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... Secondary antibodies were diluted similarly and incubated for 1 h at RT and DAPI (Sigma, D1306) staining for 4 minutes at RT was used for nuclear visualization ...
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bioRxiv - Biophysics 2023Quote: ... and treated for 2 min at RT with pepsin (40 units/mL; Sigma-Aldrich, cat. P6887) diluted in 10 mM HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were stained for 1 h at RT with antibodies against vinculin (hVin1, Sigma; 1:400) and either (phosphoTyr397-FAK (Cat# 44-625G ...
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bioRxiv - Developmental Biology 2023Quote: ... By incubating at RT in darkness for 1 hour in a 1% hydrogen peroxide (Sigma, H1009) solution in 40 % methanol in PBS ...
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bioRxiv - Immunology 2023Quote: ... Tissue was washed, incubated with DAPI for 5min at RT (1:5000, in PBS (Sigma Aldrich)) and washed ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed at RT for 15 min in 4 % PFA pH 7.4 (Sigma-Aldrich, #P6148-500G). After washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl RT mix was added (containing 25 mM Trizma hydrochloride solution pH 8 (Sigma Aldrich), 30 mM sodium chloride (Carl Roth) ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated for 1h at RT in blocking buffer containing 10% goat serum (Sigma, G9023) with 0.03 % triton X-100 in PBS and 1:50 Affini Pure Fab Fragment Donkey Anti-Mouse IgG (Jackson Immuno Research Laboratories ...
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bioRxiv - Plant Biology 2024Quote: The gels were incubated 1.5h at RT under gentle shaking with NHS-ester ATTO488 (Sigma, 41698), NHS-ester ATTO647 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... incubated the slices overnight at RT with 1:500 rabbit α c-Fos (ABE457, Merck Millipore) and 1:3000 mouse α parvalbumin (PV 235 ...
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bioRxiv - Bioengineering 2024Quote: ... in 950 µL PBS for 2 h at room temperature (RT) with Biotin-NHS (H1759, Sigma) in a 1:20 molar ratio (G-CSFR:Biotin-NHS) ...
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bioRxiv - Microbiology 2024Quote: ... Labelling was performed at RT for 1 h and quenched with 8 μl 5% hydroxylamine (Sigma). Labelled samples were acidified by adding 50 μl 1% TFA ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR reaction mixture contained 2.5 units Taq polymerase and 1 x PCR buffer (D1806, Sigma-Aldrich), 0.25 mM dNTP mix and 0.5 μM of each of the primers ...
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bioRxiv - Plant Biology 2020Quote: ... An equal concentration of cDNA from the samples comparing was used for qRT-PCR reaction with KAPA SYBR Fast qPCR Kit (Sigma-Aldrich, St. Louis, USA). For expression analysis of the genes responsive to nutrient starvation ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted at the field using quick Extract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich Co. LLC, MO, Louis. St; USA) with some modifications ...
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bioRxiv - Developmental Biology 2022Quote: ... offspring were screened for Akt1 or Foxo4 mutations from genomic DNA from tail-tip biopsies using the REDExtract-N-Amp™ Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA). PCR was performed on the purified DNA samples using primers flanking the guide RNA sites ...
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bioRxiv - Microbiology 2024Quote: PCR products obtained from the first round of amplification (COMPETE, BLOCK, CONTROL, see section 2.2) were purified using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, St. Louis, MO). The purified DNA was diluted 10-fold with DNA free sterile water and used as a DNA template for a nested PCR to attach Illumina adaptors for Illumina sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were washed several times in PBS before mounting using Fluoromount (Sigma).
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bioRxiv - Immunology 2020Quote: ... they were washed four times and 50 μl tetramethylbenzidine (TMB, Sigma-Aldrich) was added into each well ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed slowly five times with 500 µL 1X PBS (Sigma) and detached by addition of 100 µL of 0.25% trypsin-EDTA solution (Gibco ...
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bioRxiv - Genomics 2020Quote: ... PBMCs were washed three times and stained with propidium iodide (PI, Sigma). Viable cells (PI negative ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times in PBS and stained with Hematoxylin (Sigma, Darmstadt, Germany) for 1 min ...
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bioRxiv - Genomics 2019Quote: ... samples were washed three times with phosphate buffered saline (Sigma Aldrich, UK). After the final cleaning step 1 mL Trizol was added to the cells ...
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bioRxiv - Developmental Biology 2019Quote: ... washed 3 times in PBS and incubated in DAPI (1:1000, Sigma) for 20 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... cells were gently washed 3 times with DPBS (Sigma, St Louis, MO). Finally ...
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bioRxiv - Molecular Biology 2019Quote: ... dialyzed three times against 4 L of 2 mM β-mercaptoethanol (Sigma) in water ...
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bioRxiv - Biophysics 2020Quote: ... the chambers were washed three times with PBS (Sigma-Aldrich, D8537-500ML) before incubation with 0.1 g/l Neutravidin (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... After washing the chambers three times with PBS (Sigma-Aldrich, D8537-500ML), the surfaces were incubated with 1 M KOH (Fulka ...
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bioRxiv - Biophysics 2020Quote: ... The surfaces were washed three times with PBS (Sigma-Aldrich, D8537-500ML) and incubated with 10−10 M of the annealed oligonucleotides in PBS (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed 4 times using PBS with 0.1% Tween 20 (Sigma), incubated with 100µl detection antibody (TNF-α 1:100 and CCL2 0.1 µg/ml in PBS-BSA 1% ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927 ...
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bioRxiv - Neuroscience 2021Quote: ... then washed three times in PBS and coated with laminin (Sigma-Aldrich) for 4 hours (5 μg/ml for suspension culture ...
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bioRxiv - Microbiology 2020Quote: ... plates were washed 2 times with PBS-tween [PBS (Sigma, Gillingham, UK) containing 0.1% Tween-20 (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were washed three times using washing buffer (0.5% Tween 20 (Sigma) in PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed three times in quenching buffer [5mM Trolox (Sigma), 10mM sodium azide (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... After washing 4 times with PBST buffer (PBS + 0.2% Tween-20 (Sigma)) ...
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bioRxiv - Biophysics 2022Quote: ... samples were rinsed 4 times with phosphate-buffered saline (PBS, Sigma-Aldrich), incubated for 5 min with a 0.02% fibronectin (F0895 ...
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bioRxiv - Developmental Biology 2022Quote: ... washed 3 times in 1X Phosphate Buffered Saline (PBS, Sigma-Aldrich, P5493) and stored at 4°C in PBS supplemented with 0.1% Sodium Azide (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... Beads were washed two times with RBD (RIPA buffer (Sigma Cat. # R0278), 50 mg/mL Bovine Serum Albumin (BSA) ...
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bioRxiv - Cancer Biology 2022Quote: ... At their corresponding time points cells were blocked with human IgG (Sigma) and subsequently stained for quantitative flow cytometric analysis ...
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bioRxiv - Neuroscience 2021Quote: ... washed five times in PBST (PBS with 1x Triton, Sigma-Aldrich, T8787) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Larvae were next washed 3 times in PBS1X + 0.3% triton (Sigma T8787) for 10 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 3 times and then reduced with 500 μL 10 mM DTT (Sigma) in PBS for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... washed 2 times with ethanol 70% (Sigma Aldrich, St. Louis, MO, USA) and finally resuspended in water.
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bioRxiv - Cell Biology 2020Quote: ... Tissues were dounced five times using the pestle (Z359971-1EA, Sigma Aldrich) in a soft twisting motion initially ...
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bioRxiv - Genomics 2019Quote: ... were washed 3 times with a 5 mg/mL BSA (Sigma A9418) solution ...