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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The treatment group received 2 mg/ml Dox (BelaDox) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich), to induce an in vivo knockdown ...
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bioRxiv - Cancer Biology 2024Quote: ... and were selected in the F12:DMEM media containing 10% FBS and 2 μg/ml puromycin (Sigma-Aldrich) for 10-14 days.
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of purified mitochondria were placed in a solution containing 100 mM Tris pH 8.1 (T1503, Sigma), 0.25% triton X-100 (T8532 ...
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing Exo1 protein variant were pooled and applied to 0.3 mL of M2 anti-FLAG agarose beads (Sigma) in batch ...
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bioRxiv - Developmental Biology 2019Quote: ... into single-cell suspension and re-suspended in E8 media containing 10 μM Rho-associated protein kinase inhibitor (Sigma). Approximately 100,000 cells were re-plated into Matrigel-coated 6-well plates ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resuspended in a solution containing 1 % SDC and 10 mM TCEP with 40 mM CAA (Sigma-Aldrich) as reduction and alkylating agents and heated to 37 °C for 1 hour ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Eight µg of IrSPI were refolded for 30 minutes at 25°C in a final volume of 100µl containing 10µg of the disulfide isomerase protein (Sigma-Aldrich) with reduced/oxidised glutathione (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654; 0.21% L-methionine, Sigma #M9625 ...
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bioRxiv - Cell Biology 2021Quote: Expression of Flag- and EGFP-tagged proteins was induced with media containing 1 µg/mL doxycycline (Sigma-Aldrich D9891) 12-24 hours prior to harvesting ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing the S protein were pooled and concentrated using 50 kDa MWCO Amicon centrifugal filter units (EMD Millipore). During concentration ...
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bioRxiv - Microbiology 2021Quote: ... Fractions containing each DNA pol of a high purity were pooled and concentrated using a 10K protein concentrator (Millipore). Concentrated proteins were preserved at −20 °C in the presence of 50% glycerol ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Immunology 2020Quote: ... The protein-containing supernatants from cells expressing HA-I53_dn5B fusion proteins were further clarified by vacuum filtration (0.22 µm, Millipore Sigma). Prior to immobilized metal affinity chromatography ...
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bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich) and 0.1% Tween-20 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing Galectin-3 protein was treated with benzamidine sepharose (HiTrap Benzamidine FF, Sigma, Cat# GE17-5143-02) to bind and remove thrombin ...
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bioRxiv - Cancer Biology 2019Quote: ... a sample mixture of 10 μg protein and non-reducing sample buffer was loaded on a polyacrylamide gel containing gelatin (0.25 mg/ml; Sigma). The gel was then soaked (36 h ...
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bioRxiv - Cell Biology 2019Quote: ... Fractions containing monomeric protein were pooled and concentrated to around 50 μM using Amicon Ultra-0.5 ml Centrifugal Filters (Millipore), and then aliquoted and snap-frozen until further use.
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bioRxiv - Neuroscience 2019Quote: ... The proteins were then precipitated using acetonitrile (400 µL) containing 0.1% v/v formic acid (LC-grade, Sigma-Aldrich). This was done in metal and glass syringes to ensure no contamination from plastic ...
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bioRxiv - Pathology 2020Quote: ... cell lysates containing equal protein amounts were separated by SDS-PAGE and transferred to PVDF membranes (Cat.#ISEQ00010, Millipore). Membranes were sequentially probed with primary antibodies and appropriate peroxidase-conjugated secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing the proteins of interest were pooled and concentrated using Amicon Ultra-15 30K spin concentrators (Merck Millipore) before further purification using a HiLoad 16/600 Superdex 200 prep grade size exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... MBP-DIX proteins were eluted with three column volumes of lysis buffer containing 10 mM D-(+)-maltose monohydrate (Sigma) applied to loose resin under gentle rotation for 30 min ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing the protein were pooled and concentrated by centrifugal concentrators with 3 kDa molecular weight cutoff (EMD Millipore). The sample was polished by gel filtration (120 ml Superdex200 ...
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bioRxiv - Genetics 2019Quote: ... the column was inverted and bound proteins were eluted with 10 ml of buffer PT containing 0.1 mg/ml Flag peptide (Sigma). The eluate fractions were dialyzed against buffer A [25 mM Hepes-NaOH (pH 7.5) ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein of interest were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) before loading onto a size-exclusion Sepharose 200 column (Cytiva ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... prelimbic cortex were dissected and homogenized in lysis buffer (pH 7.4) containing a cocktail of protein phosphatase and proteinase inhibitors (Sigma). The samples were centrifuged at 14,000 r.p.m ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the trimeric HPIV3 F proteins were pooled and concentrated by centrifugation in an Amicon ultrafiltration unit (Millipore) with a 50 kDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated by electrophoresis through 12% polyacrylamide gels containing SDS (0.1%) and transferred to a PVDF membrane (Millipore). The membrane was blocked with 2% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... 500nM of (GlcNAc)6 were added to trigger ROS production in the absence or presence of 1μM of effector protein in a measuring solution containing 100μM of L012 substrate (Sigma Aldrich) and 40μM horseradish peroxidase (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 M NaCl) before eluting bound proteins with buffer A containing 0.5 M Methyl-α-D mannose (Sigma). Fractions containing NA activity were pooled ...
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bioRxiv - Molecular Biology 2022Quote: ... The filters were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (07-181, Millipore), p-AMPK (#2535 ...
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bioRxiv - Molecular Biology 2023Quote: ... Pure protein-containing fractions were pooled and concentrated using centrifugal spin concentrators with an MWCO of 30 kDa (Millipore). Finally ...
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bioRxiv - Microbiology 2024Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich, St. Louis, USA). After purification ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing the purified protein were pooled together and concentrated using 30-kDa cut-off centrifugal devices (Millipore). Further ...
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bioRxiv - Neuroscience 2023Quote: The eluent containing the target proteins underwent filtration using a Centricon filter with a 100 kD cutoff (EMD Millipore). The Centricon filter was then centrifuged at 1,200g for 10 minutes until the sample volume was reduced to less than 1 mL ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 µM Na3VO4) before the protein was eluted with FLAG wash buffer containing 0.5 mg/mL 3xFLAG peptide (Sigma). LIMK1 concentration was determined by running alongside BSA standards on SDS-PAGE with Coomassie staining.
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bioRxiv - Biochemistry 2023Quote: ... followed by resuspension of the dry pellets by vortexing (≈1 min) and subsequent addition of 150 μL lysis buffer (HEPES buffer containing 0.35X BugBuster Protein Extraction Reagent (Novagen) and 0.1% Lysonase Bioprocessing Reagent (Novagen)) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using centrifugal filter units (Amicon, 10KDa cut-off; Millipore, Ultra-4 UFC801024 and/or Ultra-0.5 UFC501024) ...
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bioRxiv - Biophysics 2024Quote: ... The target protein was eluted by application of 5 CVs of buffer E containing 2.5mM d -desthiobiotin (Sigma-Aldrich). Elutions were pooled ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was eluted with GFB containing 2.5 mM desthiobiotin and concentrated in a 10 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superdex 75 Increase 10/300 GL (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... The C-terminal domain (CTD) of DRH-3 (residues 940-1108) was cloned into the expression vector pET15b (Novagen), and the resulting clone carries an N-terminal His6 tag and a thrombin cleavage site ...
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bioRxiv - Biophysics 2020Quote: All used BMPs and extracellular domains (ECD) of the BMPR-FC chimeras were bought from Sigma Aldrich (Missouri, USA) and R&D systems (Minnesota ...